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TRANSAMIDATION REACTION IN DEEP EUTECTIC SOLVENTS
TRANSAMIDATION REACTION IN DEEP EUTECTIC SOLVENTS
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机译:深共熔溶剂中的磺化反应
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摘要
Herein is reported a method for the enzymatic production of a polypeptide comprising the step of incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue) or LPXTA (SEQ ID NO: 41, wherein X can be any amino acid residue), ii) a second polypeptide that has i) a glycinyl, an alaninyl, or a cysteinyl compound at its N-terminus, or ii) an oligoglycine, or oligoalanine, or a cysteine amino acid residue followed by one to three glycine or alanine amino acid residues at its N-terminus, or iii) a lysine amino acid residue within its 5 N-terminal amino acid residues, and iii) a third polypeptide with sortase A activity, in a deep eutectic solvent and thereby producing a polypeptide.
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机译:本文报道了一种酶促生产多肽的方法,该方法包括以下步骤:i)孵育包含氨基酸序列LPXTG(SEQ ID NO:01,其中X可以是任何氨基酸残基)的第一多肽或LPXTA(SEQ ID NO: :41,其中X可以是任何氨基酸残基),ii)第二种多肽,其在其N端具有i)甘氨酰基,丙氨酸或半胱氨酸化合物,或ii)寡甘氨酸或寡丙氨酸或半胱氨酸氨基酸残基,在其N端接一个至三个甘氨酸或丙氨酸氨基酸残基,或iii)在其5个N端氨基酸残基内的赖氨酸氨基酸残基,以及iii)具有分选酶A活性的第三个多肽一种深的低共熔溶剂,从而产生多肽。
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