PROCEDURE FOR PRODUCTION OF A CELL CULTURE MEDIA FOR TUMOR STEM CELL EXPANSION, METHOD, TUMOR STEM CELL EXPANSION METHOD AND USE OF A GASTRINE FREEZING PEPTIDE

摘要

process of producing a cell culture medium for tumor stem cell expansion, medium, tumor stem cell expansion method and use of a gastrin-releasing peptide. The present invention describes a culture method and medium based on the use of gastrin-releasing peptide (grp) as the sole growth factor capable of expanding tumorspheres (structures enriched in tumor stem cells) in culture. as effectively as a medium containing bfgf and egf. Specifically, the invention proposes to simplify the composition of tumor stem cell culture media and methods with efficiency equivalent to that of currently available methods and commercially available culture media. It is an advantage of the invention to use only one growth factor as a principle biologically active culture media. The present invention is in the fields of cell biology, molecular biology and applied health biotechnology and experimental oncology.