PRODUCTION OF ROUGH-TYPE SALMONELLA ENTERITIDIS AND THE GENETIC MODIFICATIONS THEREOF FOR USE AS AN AVIAN VACCINE

摘要

The present invention relates to a strain of Salmonella enteritidis 3934vac from which the waaL gene has been deleted in order to obtain a rough phenotype (3934vac DwaaL), as well as to the method for obtaining same and to the oligos used, with a view to reducing toxicity and maintaining immunogenicity for the use of same as a vaccine. The present invention also relates to a strain of Salmonella enteritidis 3934vac DwaaL, i.e. of the rough-type, which has been modified to express the fiber gene of type-I avian adenovirus, as well as to the method for obtaining a strain of Salmonella enteritidis 3034 vac DwaaL which expresses a fiber gene of Ava-I. The invention further comprises the development of a novel avian vaccine against the AvA-I virus, which is live, recombinant, effective and harmless and which was developed using a process involving inserting and integrating Ava-I fiber genes into the chromosome of a non-pathogenic attenuated strain of Salmonella enteritidis bacteria.