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Amplification Technology Using Dual Enzyme Cascade Detection
Amplification Technology Using Dual Enzyme Cascade Detection
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机译:使用双重酶级联检测的扩增技术
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摘要
Diagnostic assays and methods employ a dual-enzyme cascade system comprised of two enzymatic amplifiers and two molecular probes, by (a) incubating a target enzyme with a target enzyme substrate to liberate a thiol; (b) incubating the thiol with a disulfide inactivated amplification enzyme to activate the amplification enzyme in an interchange reaction of the thiol and the disulfide; and (c) incubating the activated amplification enzyme with an amplification enzyme substrate to generate an amplified signal.
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