METHODS FOR DETECTING INCORPORATION OF A REVERSIBLE TERMINATOR 3? -O-DEOXYRIBONUCLEOTIDE TO PERFORM A SEQUENCING REACTION BY SYNTHESIS, TO PERFORM SEQUENCING BY EXTREMELY DEOXYRIBONUCLEOTIDE SYNTHESIS? OF AN INITIATOR EXTENSION PRODUCT AND FOR SEQUENCING A NUCLEIC ACID, DNA ARRANGEMENT, AND, KIT

摘要

the invention provides compositions and methods for nucleic acid sequencing and other applications. in synthesis sequencing, unmarked reversible terminators are incorporated by a polymerase in each cycle, then marked after incorporation by binding to the reversible terminator of a directly or indirectly labeled antibody or other affinity reagent.