APPROPRIATE POLYPEPTIDE FOR THE DETECTION OF ANTIBODIES AGAINST ZIKA VIRUS, METHODS OF PRODUCTION OF POLYPEPTIDE, OF DETECTION OF ANTIBODIES SPECIFIC TO ZIKA VIRUSES AND OF DETECTION OF ANTIBODIES AGAINST ZIKA VIRUSES, USE OF POLYTRAYE AGENTS

摘要

the present invention relates to a polypeptide suitable for detecting antibodies to Zika virus in isolated biological samples, comprising a specific amino acid sequence of the ns1 wing domain of Zika virus and its variants, in which no virus-specific amino acid sequence Additional zika is present in the mentioned polypeptide. this polypeptide does not show immunological cross-reaction with antibodies raised against antigens with a structural relationship of tick-borne encephalitis virus, dengue virus 1-4, west nile virus, yellow fever virus or Japanese encephalitis virus, but reacts immunologically with antigens raised against full length Zika virus ns1 antigen. also described is a method of producing immunoresponsive and soluble zika virus ns1 polypeptide, as well as specific antibody detection methods and kits for zika virus in isolated samples.