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Yarrowia lipolytica yeast preparation with high content of zinc, selenium or chromium and method for producing it
Yarrowia lipolytica yeast preparation with high content of zinc, selenium or chromium and method for producing it
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机译:高锌,硒或铬含量的解脂耶氏酵母酵母制剂及其生产方法
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摘要
The notification shall cover the production of yeast biomass with a high zinc, selenium or chromium content, namely:that Yarrowia lipolytica is grown periodically in a medium containing: glycerol, favourable in concentration from 40% to 100%, ammonia water, favourable in concentration from 20% to 40%,maize starch, preferably 94% to 100%, magnesium sulphate, preferably 95% to 100% and monopotassium phosphate,favourable in concentration from 95%to 100% and metal salt selected from: sodium selenium (IV), favourable in concentration from 95% to 100%, sodium selenate (VI), favourable in concentration from 95%to 100%,chromium chloride (III), preferably in concentration from 95%to 100%, or zinc sulphate (II), preferably in concentration from 95% to 100%, the holding being kept at temperature 30oC,with pH maintained at a level of 4 to 6, preferably 5, with constant ventilation from 0,36 to 0,6 vvm and mixing with the rotating mixer at a speed of 700 to 800 rpm, for at least eight hours,and then separated the resulting biomass, preferably by centrifugation, subjected to drying to reach humidity below 5% of the water content and, where appropriate, packaged.The notification shall also include the manner in which:that the yeast strain Yarrowia lipolytica SKOTAN deposited under the Budapest Treaty in IBPRS under KKP 2018 p is grown.The product concerned is also: yeast preparation in the form of dry yeast biomass Yarrowia lipolytics enriched with zinc containing 7000 -9000 mg Zn /kg dry biomass,yeast preparation in the form of dry yeast biomass Yarrowia lipolytics enriched with chromium containing 18 -40 mg Cr /kg.The yeast preparation in the form of dry yeast biomass Yarrowia selenium -enriched lipolytics (VI) containing 50 -80 mg Se (VI)/kg.The yeast preparation in the form of dry yeast biomass Yarrowia selenium -enriched lipolytics (IV) containing 200 -270 mg Se (IV)/kg.
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