MULTIPLEX TARGET GENE EXPRESSION INHIBITION SYSTEM BASED ON SYNTHESIS REGULATOR SRNA AND METHOD OF PRODUCING SAME

摘要

The present invention relates to a multiplex expression inhibition system based on a synthesis regulator sRNA and a method of producing same, and more specifically, to a combination of vectors comprising a variety of antibiotic-resistance genes, an origins of replication, and a synthesis regulator sRNA; a method of producing same; an sRNA library consisting of said combination of vectors; and a method of screening for a bacterial strain producing an useful substance by using same. The multiplex target gene expression inhibition system according to the present invention is a gene expression regulation system based on a synthesis regulator sRNA that, unlike existing gene knock-out methods, can effectively inhibit the expression of a specific gene without altering the sequence of a target gene; comprises various vectors and thus can be used compatibly with other plasmids usable in prokaryotes; and is designed to enable multiplex introduction, and thus is advantageous in a fast and efficient production of recombinant microorganisms. Furthermore, the present invention provides a technology that enables simple and fast transferring a genome-level synthesis regulation sRNA library to a new platform, and said technology is useful in selection of recombinant microorganisms producing useful substances in high capacity. Accordingly, the present invention is useful in the production of recombinant bacterial strains capable of dramatically increasing the production efficiency of a variety of medically useful metabolites.