Gene therapeutic DNA vector based on VTvaf17 gene therapeutic DNA vector, carrying the target gene selected from the group of genes NOS2, NOS3, VIP, KCNMA1, CGRP, to increase the expression level of these target genes, the method for its preparation and use, strain Escherichia coli SCS110-AF / VTvaf17-NOS2, or Escherichia coli SCS110-AF / VTvaf17-NOS3, or Escherichia coli SCS110-AF / VTvaf17-VIP, or Escherichia coli SCS110-AF / VTvaf17-KCNMA1, or Escherichia coli SCS110-AF / VTvaf17 gene therapy DNA vector, method for producing it, method for the industrial production of gene therapy DNA vector

摘要

The invention refers to genetic engineering and can be used in biotechnology, medicine, and agriculture for the manufacture of gene therapy products. Gene therapy DNA vector based on the gene therapy DNA vector VTvaflV carrying the therapeutic gene selected from the group of NOS2, NOS3, VIP, KCNMA1, and CGRP genes is constructed in order to increase the expression level of this therapeutic gene in humans and animals, while gene therapy DNA vector VTvafl7-NOS2, or VTvaflV- NOS3, or VTvafl7-VIP, or VTvafl7-KCNMAl, or VTvafl7-CGRP has the nucleotide sequence SEQ ID No. 1, or SEQ ID No. 2, or SEQ ID No. 3, or SEQ ID No. 4, or SEQ ID No. 5, respectively. Each of the constructed gene therapy DNA vectors, namely VTvafl7-NOS2, or VTvafl7-NOS3, or VTvafl7-VIP, or VTvafl7-KCNMAl, or VTvafl7-CGRP has the ability to efficiently penetrate into cells and express the therapeutic gene selected from the group of NOS2, NOS3, VIP, KCNMA1, and CGRP genes, respectively, and cloned to it due to the limited size of VTvafl7 vector part not exceeding 3200 bp. The gene therapy DNA vector contains no nucleotide sequences of viral origin and no antibiotic resistance genes, which ensures its safe use for gene therapy in humans and animals.