Method for separation of dopaminergic neural cells and pharmaceutical composition comprising dopaminergic neural cells for treatment of Parkinson's disease

摘要

#$%^&*AU2019240547A120191205.pdf#####(19) All 1i-7]-"1 f)4j ]j. a11111111111111111li1111l7iili (10) --I 7lld (43) A]I -- 7l 1''-07 2019 l 116 7 o (07.11.2019) WIPOIPCT W O 2019/212201 Al (51) 14 l j *lT': (PARK, Sanghyun); 01195 A]i&^1 4'H °UN -V27 C12N5/0793 (2010.01) A61P25/16 (2006.01) 70 19, 210-1103, Seoul (KR). 7J (KIM, Jongwan); A61K 35/30 (2006.01) 02814 Ai a'A1 , " i i 267 8,206-507, Seoul (KR). PCT/KR2019/005058 (CHO, Myung Soo); 01488 A]1 A] (21) 1t 350, 402-1209, Seoul (KR). (22) #]1-%i: 2019 V 4 N 26 V (26.04.2019) (74) FIZ ?l: 'rI (YOON, Dae Woong); 08793 Ai A^ A (25) -11 -: 4Oi T 1922,301-,Seoul(KR). (26) `67}] 0-: W-i (81) A] 51 N ( 1 A A17}1 Mq 7}1d£ W (30) -- ' • -- :l 1-V -i -&I}0): AE, AG, AL, AM, AO, AT, 10-2018-0050918 2018 V 5 N 2 " (02.05.2018) KR AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, 10-2018-0048784 20198k 45 25 (250.201) K(R CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, 10-2019-0048784 2019V4 4 N 25 (25.04.2019) K(R EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, HN, HR, HU, (71) Oil: (T) 0ll 1 MO9A q- (S-BIOMEDICS) [KR/ ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, KR]; 04797,A1 A^1 2 V T 126 728, 4, Seoul LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, (KR). MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, (72) 1¶}: 7dl94 (KIM, Dong-Wook); 04095 A ~ SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, T {1T X 107, 102-702, Seoul (KR). r -- (YOO, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Jeong-Eun); 11685 71 ]5 ^ Ma 681-11, Gyeonggi-do (KR). 01% (LEE, Dongjin); 06077 A (84) Al -( 1 AA]171} M± , 7}12 ] 1 ~714 NW. 609, 2-507, Seoul (KR). 4t 1E1 I 0j1O}1): ARIPO (BW, GH, GM, KE, (54) Title: METHOD FOR ISOLATING DOPAMINE NEURONS AND PHARMACEUTICAL COMPOSITION FOR TREATING PARKINSON'S DISEASE, CONTAINING DOPAMINE NEURONS ISOLATED USING SAME (54- -1o 11o 6:5 Rj ILI Al K 1] 01T I 1 oN- 0 -9--at0101 -V 9 4II All -K-NAp - ~~4-at P = 11 -- j~ 50* 30 * 40 20 30 200 10 0 - -- -, ---- Unsorted TPBG+ Unsorted TPBG+ *P0,05, **PC0.01,** P0.001 (57) Abstract: The present invention relates to a method for isolating doparme neurons and a pharmaceutical composition for treating Parkinson's disease, containing doparme neurons isolated using same. The method for isolating doparme neurons comprises a step for isolating trophoblast glycoprotein (TPBG)-positive doparme neurons, such that the doparme neurons isolated by the method have increased cell efficacy when transplanted and enhanced transplantation safety so as to be effectively used for cell transplantation for Cthe treatment of Parkinson's disease. Al~_q _VA ?& l WWM , 1 It} 71 - Ail 1 " - E,l t TPBG(Trophoblast glycoprotein)-O, } JL - 01T15Y ~ I'" 0 ls1a 0 3TlJ01 ;A -R71Z.111-] LN l 1TO--IA -- F(JlW O 2019/212201 A 1 ||11||||||||||||||IHI|I|111|1|I|I||I|HI |I|IDI |ID ||I|||||||||||||||||||||||| LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), ^ elA]} 4(AM, AZ, BY, KG, KZ, RU, TJ, TM), ei (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). o71]: - -%AlLA1i7(- Al 21-(3)) All A] 1 A4 11( 5.2(a))