METHOD FOR SIMULATING AND TREATING AN OPEN FRACTURE OF A TUBULAR BONE IN AN EXPERIMENT

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to clinical-experimental traumatology and orthopedics, and can be used to study the effectiveness of the effect of the antibacterial preparation on the wound process and the experimental animal body as a whole in treating an open fracture in an experiment. Method involves forming an open bone fracture in an experimental animal and performing intramedullary osteosynthesis. Formation of open bone fracture is performed from mini-access after preparation of direct and deflecting muscles and skeletonisation of guinea-pig femoral diaphysis. Transverse osteotomy of the femoral shaft is first performed on a half-diameter of the bone, followed by manual osteoclasis. After the open fracture model is formed, retro-intramedullary osteosynthesis is performed by inserting 1.5-mm-diameter K-wire into the proximal segment using an operating drill until it appears above the skin in a projection of greater trochanter, comparing the proximal and distal bone fragments. Wire is antegrade introduced into the distal segment until it appears above the skin in the projection of the femoral condyles. Further, the ends of the pin are fixed on the skin with rubber fixators and arched to exclude fixator migration; perioperative antibiotic prophylaxis is performed for 24 hours by intramuscular introduction of three cefazolin injections, wherein the first injection is introduced immediately after forming an open fracture model, and the next two are performed after osteosynthesis with gap of 8 hours.;EFFECT: method provides simulating an open fracture of a tubular bone maximally close to actual trauma conditions, and preventing postoperative suppurative-inflammatory processes in treating a fracture by performing transverse osteotomy without damaging surrounding tissues.;1 cl, 4 dwg, 1 ex