一种抗虫性的苏铁毒蛋白基因、表达载体及应用

摘要

本发明属于基因工程技术领域，公开了一种抗虫性的苏铁毒蛋白基因、表达载体及应用，所述抗虫性的苏铁毒蛋白基因及其同源基因的核苷酸序列为SEQ ID NO.1，SEQ ID NO.2，SEQ ID NO.3，SEQ ID NO.4，SEQ ID NO.5。本发明提供的毒蛋白基因来自苏铁，外源表达注射发现其对小菜蛾与棉铃虫幼虫具有毒性作用。将其导入到棉花中，使棉花获得草地贪夜蛾和棉铃虫抗性，且后代能够稳定遗传。本发明为抗虫植物的研制提供了一种新的抗虫基因，不仅可以使棉花等受体获得多种机制的抗虫性，还利用基因互补的方式加强转基因棉花的抗虫能力，延缓害虫产生抗性，拓宽了棉花抗虫谱，使其获得能够高效杀死主要害虫的广谱抗虫性。

说明书

技术领域

本发明属于基因工程技术领域，尤其涉及一种抗虫性的苏铁毒蛋白基因、表达载体及应用。

背景技术

小麦、玉米、棉花等作物可持续性发展是保障我国粮食安全的重中之重。其中棉花不仅是世界上最重要的天然纤维作物，也是重要的油料作物和高蛋白粮食作物。

全球每年多达40％的农作物产量因虫害而损失，造成的损失至少为700亿美元。为了抵御害虫侵袭，科学家们在抗虫基因挖掘与抗性机制解析方面取得了一系列重要进展。目前，研究成功的抗虫基因主要有苏云金芽孢杆菌杀虫基因、豇豆胰蛋白酶抑制剂、慈菇蛋白酶抑制剂、淀粉酶抑制剂、营养杀虫蛋白、胆固醇氧化酶、雪花莲凝集素、半夏凝集素、尾穗苋凝集素及蝎毒基因等昆虫神经毒素。在棉花上应用最为成功的是郭三堆等研制的双价转基因抗虫棉新品种，其遗传转化的抗虫基因为Bt杀虫基因GFMCry1A和豇豆胰蛋白酶抑制剂基因Cpti。

虽然现有转抗虫基因棉花能有效控制鳞翅目、鞘翅目、同翅目害虫，但由于这些基因在棉花整个生长期内一直表达，这就有可能使害虫易于对其产生抗性，从而成为转基因棉花发展中的最大威胁。其中，澳大利亚转基因抗虫棉已然对棉铃虫无效。

目前农作物抗病虫育种可用抗性基因资源匮乏，急需挖掘新的抗病虫基因，并利用基因互补的方式加强转基因作物或植物的抗虫能力，同时也应注意延缓害虫产生抗(耐)性。

发明内容

针对现有技术存在的问题，本发明提供了一种抗虫性的苏铁毒蛋白基因、表达载体及应用。

本发明是这样实现的，一种抗虫性的苏铁毒蛋白基因，所述抗虫性的苏铁毒蛋白基因的核苷酸序列为SEQ ID NO.1。

本发明的另一目的在于提供苏铁中所述抗虫性的苏铁毒蛋白基因的同源基因，所述同源基因的核苷酸序列为SEQ ID NO.2，SEQ ID NO.3，SEQ ID NO.4，SEQ ID NO.5。

本发明的另一目的在于提供一种由所述的抗虫性的苏铁毒蛋白基因经密码子优化得到的苏铁毒蛋白优化基因，优化物种为棉花，所述苏铁毒蛋白优化基因的核苷酸序列为SEQ ID NO.6，SEQ ID NO.7，SEQ ID NO.8，SEQ ID NO.9，SEQ ID NO.10。

本发明的另一目的在于提供一种编码所述的抗虫性的苏铁毒蛋白基因的蛋白质，所述蛋白质的氨基酸序列为SEQ ID NO.11，SEQ ID NO.12，SEQ ID NO.13，SEQ ID NO.14，SEQ ID NO.15。

本发明的另一目的在于提供一种编码所述的抗虫性的苏铁毒蛋白基因的杀虫应用或杀虫剂制造，应用范围包括但不限于棉铃虫、小菜蛾、草地贪夜蛾、线虫等植物或作物害虫。

本发明的另一目的在于提供一种应用所述的抗虫性的苏铁毒蛋白基因构建得到的植物或作物表达载体，所述表达载体的构建方法包括：

线性化载体制备：使用限制性内切酶BstBI和MluI双酶切pVM062-3*FLAG载体，使环状载体线性化。

带有同源臂的目标序列扩增：

以正向引物：

5’-gggcggtaccccgggttcgaaATGGTTATGGTAATTGACTATCCAAGTC-3’；

反向引物：5’-cactagttaattaagacgcgtCTATGTCATGCGTAGAATGGCCC-3’

为引物，对苏铁毒蛋白基因进行PCR扩增。

载体酶切产物与PCR产物的检测与回收：琼脂糖凝胶电泳检测目的基因片段与载体酶切产物，使用

胶回收产物重组：使用

进一步，所述扩增体系为：KOD One

所述PCR的程序为：PCR的程序为98℃5min；98℃30s，50℃30s，68℃3min，30个循环；68℃10min，4℃保存。

进一步，所述转化方法包括：

(1)在冰上融化Trans1-T1感受态细胞，静置2-5分钟，观察细胞是否已解冻，用手指轻弹管底1-2次；在50μL细胞中加入2μL的重组产物，轻轻弹动离心管壁混匀后，在冰上放置30min；

(2)42℃水浴中热激30s后马上转至冰上冷却2min；加入450μL常温无抗LB液体培养基后，于37℃摇床中250rpm培养1h；

(3)5000rpm离心2min，收集菌体，留100μL液体重悬，均匀涂布于含有卡那霉素抗性的LB固体平板上，37℃培养箱过夜培养。

本发明的另一目的在于提供一种应用所述的抗虫性的苏铁毒蛋白基因的植物或作物的抗虫方法，所述植物或作物的抗虫方法是将含有目标序列的植物表达载体导入到植物或作物细胞内，并由此获得对害虫有抗性或杀伤能力的转基因植物或作物及其后代，包括种子和任何部分的植物或作物组织；或者通过同源或异源表达的方法获得该蛋白施用于植物或作物，并由此植物或作物获得虫害防御能力。

其中，所述植物或作物包括但不限于棉花、玉米、小麦和水稻等。

本发明的另一目的在于提供一种转基因棉花植株的苏铁毒蛋白基因表达检测方法，所述转基因棉花植株的苏铁毒蛋白基因表达检测方法包括：取转基因棉花纯合株系叶片，提取叶片总蛋白，用于Western Blot检测；具体包括：

(1)取新鲜样品1～2g，将样品放在研钵中用液氮研磨；研磨后置于10mL试管中，并加入蛋白提取液4℃摇匀30min；

(2)于12000rpm 4℃离心20min；提取上清液，样品制备完成；

(3)取上清加入Loading Buffer，98℃煮5～10min后用SDS-聚丙烯酰胺凝胶电泳法测定蛋白质表达情况。

进一步，所述蛋白提取液包括0.01M PBS pH＝8，1mM PMSF，2mM DTT。

结合上述的技术方案和解决的技术问题，本发明所要保护的技术方案所具备的优点及积极效果为：

针对农作物抗病虫育种可用抗性基因资源匮乏，本发明提供了一种新的能够使植物或作物(包括但不限于棉花、玉米、小麦和水稻等)产生抗虫性的苏铁毒蛋白基因，本发明提供了一个明确的转基因棉花株系，其具有草地贪夜蛾和棉铃虫抗性。

本发明提供了一种新的抗虫性的苏铁毒蛋白基因，构建相关植物表达载体，利用农杆菌介导法转化棉花，获得转基因棉花株系，具体步骤包括对抗虫性的苏铁毒蛋白基因进行克隆，测序分析；构建所述基因的超表达载体；采用农杆菌介导法进行棉花遗传转化；其中，所述超表达载体中抗虫性的苏铁毒蛋白基因是由35s启动子驱动。本发明提供的毒蛋白基因来自苏铁，将其导入到棉花中，使棉花获得草地贪夜蛾和棉铃虫抗性，且后代能够稳定遗传。

本发明能够进一步提供或利用上述抗虫基因获得其它转基因作物和相应的种子，也可以利用有性杂交的方式将本发明的抗虫基因转入相同背景的其它品种或相近物种，以达到加快各物种抗虫种质创新的效果。

本发明描述了小菜蛾、草地贪夜蛾、棉铃虫等农业主要害虫注射苏铁毒蛋白及转基因植株喂养存活情况，表征如下：

(1)取龄期一致的三龄第二天的小菜蛾(平均体重1.33mg/头)。每头小菜蛾注射2.03μg苏铁毒蛋白，设置对照组，注射等体积(207nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果表明注射苏铁毒蛋白后，小菜蛾平均死亡率为89.30％，显著高于对照组的死亡率7.78％。

(2)取龄期一致的二龄第二天的棉铃虫(平均体重是2.06mg/头)。每头棉铃虫注射3.38μg苏铁毒蛋白，设置对照组，注射等体积(345nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果表明注射苏铁毒蛋白后，棉铃虫平均死亡率为37.78％，显著高于对照组的死亡率5.56％。

(3)取龄期一致的二龄草地贪夜蛾(平均体重1.01mg/头)，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫，25℃培养4天统计死亡率。结果表明喂食转基因棉花4天后，草地贪夜蛾的平均死亡率为61.11％，远高于喂食“百棉1号”的平均死亡率24.07％和喂食TM-1的平均死亡率12.96％。

(4)取龄期一致的二龄棉铃虫(平均体重3.9mg/头)用于棉铃虫的取食实验。实验前，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫，25℃培养4天统计死亡率。结果表明喂食转基因棉花4天后，棉铃虫的平均死亡率为59.26％，远高于喂食“百棉1号”的平均死亡率29.63％和喂食TM-1的平均死亡率14.81％。

本发明组装了苏铁高质量基因组，通过基因组分析发现了水平基因转移现象，即苏铁基因组中发现了来自于真菌的基因。通过分析发现该基因编码蛋白含有细胞毒素结构域，具有杀虫效果。而且水平基因转移是一个自然的转基因过程，这些转移的基因通常对植物无害，因此本发明选择该基因作为抗虫候选基因。

本发明苏铁毒蛋白基因来源于发明人组装的苏铁基因组，通过基因组分析并提取出该基因序列，设计引物并进行PCR克隆得到。

本发明所提供的苏铁毒蛋白基因基因可显著提高植物(包括但不限于棉花、玉米、小麦和水稻等)抗虫性的一个基因，由于其来源于植物自身，转化到其他植物后一般不存在毒害效应。该基因转化棉花后，不仅可以使棉花同时获得草地贪夜蛾和棉铃虫抗性，还可以利用基因互补的方式加强转基因棉花的抗虫能力，拓宽了棉花抗虫谱，使其获得能够高效杀死主要害虫的广谱抗虫性。本发明成功克隆了苏铁毒蛋白基因，提供了该基因的核苷酸序列和编码蛋白质氨基酸序列，为植物抗虫种质的创制及新品种的选育提供理论基础和应用参考。

本发明的技术方案转化后的预期收益和商业价值为：本发明的技术方案转化后的预期收益与后续转基因棉花品种的审定和推广面积有关，我国抗虫棉面积自2006年以来，稳定在350万公顷左右，本发明将通过传统遗传实验，培育不同背景的抗虫棉，并积极推进审定棉花抗虫新品种，商业价值十分可观。

附图说明

为了更清楚地说明本发明实施例的技术方案，下面将对本发明实施例中所需要使用的附图做简单的介绍，显而易见地，下面所描述的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下还可以根据这些附图获得其他的附图。

图1是本发明实施例提供的苏铁毒蛋白基因的PCR扩增结果示意图。

图2是本发明实施例提供的构建的植物表达载体示意图，利用35S启动子驱动苏铁毒蛋白基因，该植物表达载体带有壮观霉素抗性基因。

图3是本发明实施例提供的转基因棉花植株示意图。

图4是本发明实施例提供的对棉花遗传转化植株叶片中苏铁毒蛋白基因的表达进行PCR检测的示意图，图中可以清晰看到苏铁毒蛋白基因在棉花叶片中表达。

图5是本发明实施例提供的对棉花遗传转化植株叶片中苏铁毒蛋白基因的表达进行Western Blot检测的示意图，图中可以清晰看到苏铁毒蛋白基因在棉花叶片中表达。

图6是本发明实施例提供的对棉花遗传转化植株叶片进行抗虫检测的示意图，图中可以清晰看到苏铁毒蛋白基因显著抑制草地贪夜蛾和棉铃虫的生长发育。

具体实施方式

为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。

针对现有技术存在的问题，本发明提供了一种抗虫性的苏铁毒蛋白基因、表达载体及应用，下面结合附图对本发明作详细的描述。

一、解释说明实施例。为了使本领域技术人员充分了解本发明如何具体实现，该部分是对权利要求技术方案进行展开说明的解释说明实施例。

本发明所涉及的KOD One

实施例1：克隆苏铁毒蛋白基因

1.苏铁RNA模板的获得

取苏铁嫩叶，使用植物RNA提取试剂盒提取RNA。取苏铁嫩叶约1g，液氮速冻，放入研钵中研磨至细粉状，之后按照植物RNA提取试剂盒说明书进行RNA提取。RNA浓度用NanoDrop可见光分光光度计(Thermo Fisher，美国)测定。

取刚提取的苏铁RNA，按照反转录试剂盒说明书将RNA反转录为cDNA。

利用反转录得到的cDNA为模板，SEQ ID NO.16：以正向引物：5’-ATGGTTATGGTAATTGACTATCCAAGTC-3’；反向引物：5’-CTATGTCATGCGTAGAATGGCCC-3’为引物对苏铁毒蛋白基因进行PCR扩增，扩增体系为：

KOD One

上游引物(10μM each)1.5μL

下游引物(10μM each)1.5μL

模板2μL

灭菌水20μL

总体积50μL

PCR的程序为98℃5min；98℃30s，50℃30s，68℃3min，30个循环；然后68℃10min，4℃保存。PCR产物用琼脂糖凝胶电泳检测(见图1)，之后用

atggttatggtaattgactatccaagtctcagagcttcgttatatgccactaagagtgcggtcccatgtcgcccgcttccgggcaatccagagcgggtgtcgcgccgggtatcacgcagaggcgcgggagagatcgggtcccatgcagatttctggatctcccgcgccaaggcatccgccatccggattttcccgcgtcaagagggtccgctccgtgaatcccggagaagattctatcttctccgcgaagcggtgttcaccggccgcaggaccgcctgggccgctaatccagcggcccaacgggcagcatgggaccgcaagagatcccctttcttcggaaggtcttccgatgatattcattcattcagcaagaatccagaagaattcttgactcaaaacactctcagctgcagtttattaactcgcaccgctcagaccgtcacaaaaagcccagtattgcccgcccaaggttgggtcaaagtgggtcgtgtgccaggaagaggagacgctctctttgttgagctggttgccgagaaatcagatgagacagtgaaggcgtacttacttggctacaatggtaagaacgactcccccgagccggcatacgttgatattccaaagaggggcgccgagaacggaacatttttgtttaccgtggatctctcgggctgctccgttgttgtgacacagttggacgagcagacataccgtgttttccacgacaaacgggtgaacagctccattctgtacgacaacgtcgtaatgtatgtggatttcaatcactaccgctatcccagagcccacggacacgggagagatacgggacaagcggtcgtctgtatgcaattcgccgacggagtgtggaaattgtttctccagcggcacgagacggcgaattctctcccaaagatatggtcccgtggcgatggcaaaccggagattttcagcgacgccgaaccccgtccacatatggaagatggcataaaggaatttctcaatcggcgtcgagctttgcaagacgaattgaagcacttcgccaacacgcttgaagtggacccggacatagtgtccaacgctgtcgacggcgaatacgaaggcggccagttttccaggaacgacgatgcaattcgtggatggaacgaattacgtgcaagcatgagacagaaaatggctgaaaggaagaaactgttgcaaacaaagaaaggtatgctagactcattcaacaacccggatcaccccctctataaatttcgcaaacaagtgctcgacgagctcaaggaaatgagtgcatacgacaacaccttggaggcatccgagattgcggaccgtacttggctgtggttggagatcaaaaaagccaaaggtacgaatgcagtggtaaacagtggagcagccccgaagcagccgagtcttaccgttggtgaacgctacgcagagatggaggcggccctttggatgcgtagcgacacagcggcgtttgagcaagggtatgaatcttatcaaaccgttcgcgtgccgggttacaatgaagatatgacatccattgcaatgaagaagttgttcgttgagagcagtctgactgacgagcagctgggcgccttgtgcaagcgtatatccattgctagcgatcaagagaaccatcttctgacacagaagacgcaggagattgagagcactttcaaacaattttcagatggcagctttttggagtcggcgcctcaggatttcttcctgagacgagtcggtgataaatctggaggacgctgctatcctcttgtaagggtaatggccgtcgcactgtctgaaggagaagacgttgcccagaattttatggagaacctgaggacattgtctaggaatccagagtattcaggcgaatcggcaagttcacaagccacaaatgcagggttgttcagagatgccctggccggcctgcacgcgaatgtggaagccattgccgcatctgagaaactacctgctgtgtttggactgcccgatgttgctacaatgttgagccaagcgagcgaaagcaaggtttacgctttgaataccgaaacacacagcatgctggttgcggtgattgtagacaaacagagtctggcgcgcaagtattacttctatgatcccaactttctcatggccggtttctccgacataactcgtttctcggaagccctcaagtatcactttgtgaatctccagttcgccggaaactacaaagcgtttggcacagcggaggctccccagtttaacctggtccttattgatacgaccgccatggaggatgcgcatgttgacggagggatcaccgtgaaacaattgagcgagggcgttgatcttactaatgtctttgacagcaccaaaaaggctcgtgtggtaactgaggcagcagctcgcttggctgaagaccaacaactgcgcactggcctcaccataatagaggctgaccagtgggcaaacaggtggcaagatgccgtcatacgattgaaactcaaggaaggattaagtggagagtggatgccattgctggagaaaatgcaagaaaacgcagatggttcttatcagattgatttcatcaacaccaagaatactggggaaatccgtggggtttccactagagatgcgacgttcaaagaattcaaatcctacctggataaaaatttagacgtattgcataaggatttcatctttcaggatggagttttcaaaccactggagggagagcccattgacgggttgaatgcaatgttcgcggtccaaatgattatagattggttcaaccgggggactaatgctgaggaagatgcggcctcaggatccaccaatgcccaactttccctagcgattaaagtgcacagctacattaacatggcccaagtagcgcatactgcagttacagatgttgccaaggtatacaaccttgtaaagtcagcactgtatggcgagcaggtagctggggagacatcgctgttcacatttgggaaggcgttgaaatttgcagcaacagaagggttgggagtcttactaggggcgtcctttgtgatcttggatgcctaccagctgtccctcgtggaaaacgatgtgcagaaggccattatcggcacacaactagcctttgattccgcaagctttctagcgggtattggtgcaattgttgctggagcatcgggcgtgggcatgattttgggcggagctggcgtggtattgggaggcctgggagttggctttggagccttagctcaggcattcggggaaatcacagcagacgcccaactggtgggcaagtacttcgcactcttggacgaggcataccgcggtggcggatacagatacgaatctaaagacaaggcattgatgcctcttgcaggagcagtcatcgacaaaatacgactggccgacaaacatctccaagttcatttcgacagccaattcatatatcgaacccatcatggcagcacaggatccggcaaaattaattacttcttctgggctggcgacatgccgcagatggtccacgatcgctcccaagctatcaatgttcgagagcaaattgggtataagaacgataatgctactttggagctaccggaggatgcctctgatctgatcctgcctgccacgcccaagtcatttatcacttaccgatacgagattctaccattcgctacttcccgccacgatacgggtttcgatgtcattagacgcctggaagaagactacagattcgattacgatttctacatcttcccatctgaatacacaatcagaaccatcaaacacgagtacgtagaaacaaaggtggcagtagtcctggataggcgctctttgcgactgggagcacctgagcttctcaaggagcaacatggctatttgatctatgagatcgaggggaccggaggacaatacgttatatgtcttaaccagggatccactgtcacactgaagaccaccggatcagaggcaaataaaacattatggatacttgatgcccgacaactcattgcaggcaacgattcaatcacggtctctcaagatagcatcaccatcggcagcacaacagtccatctccgtaaccaagatttcgattgcctacagatcgtcatgagtcgggaggagcttctccaagtggacttggcgtctggagaagcccgcgtgttttcagaagatgccagtaaatggctcgacaccaaagaaacaatccatgaacatctccataagttggctgaagaacacaggttgcatggccagtttgtagtggtgaacaattattatcgtggagtgggccgtgcctactatgatgctgctcacgatcggatgttgtatacagatgtggaagatgaaactctgactcgggatgctgttcttgccggcgttacaggcgaagatgcctacttctacaatgtaaagcagaatgccctgtggcgagtgaatgcaggcacacaccaagtaataaccaaatactatccgacgttactggaggaccctgacagcaacatggcaagagttgagcagcatggcaacatcgtcgtgatggccatgagcaggctcaaccacccggacaagggagtaaatggaatcatctatctcgttcatcaagaaagcatcgtgcttactgctgtgatcggcaattcagagatgctcaaacgtcttcgccaaacgagtacaattagcttaacagaattgcgaggtgtcaatgacatggctctggtttccacgatggacagattacccggccaaaaggttgtcgctaccaacgctgagtttgtgacacttctggctaaggatgagcaaggagccaatatcaaggagcagacggtctattgccaacaaggtacgggccagaccaacgatacatctattgaaagcatcccgattccagggctctctaatcttttaaccgtgtacggtgaagtattcgctacaactaatgatggtcgcatccttcgcctgcttggggacggctcaacatgtctcgagggcatcaatgagcattggctcatcaagcatccaaaatggtggcaggacttggctaccttggaggcagagaaagccacgacagttgcagtattcggtgttagagacctacaaaatgcagtggtgccaatttggtaccacaacggaaagatcgcggtggcctcatcaaaactgtatggcaaacaactgcaatttttggggtttaacagcaacggtgaagcttggttgttcgacttggacgacggcattctgtatagccagccgcttgtggatagctttctgtatgcaatctttagtttggacgggaagcttattgcaccaatctttattccacaagtggagcaaatgctcagcggccagcatctcagaaaccctgtcatcgtggacggccgtttgcatgtgactaccatagacggtgttgttgttggatttgacaagaagtcgccaactctggttgcagtgaacaaggcctggcagatatctcgtcagaaccgattagaagaggccctcggaaacctgctgtccaattggtctgatcacaatgatgcgattgtgttgcagggtgatccagaatcagcgccggcatggtattatgttccatcaagagaaatagtgaccgccaatggtctaacatgggcggatcatcctctatggctaggaatggctgtcggtgctgcaacgccatacatctacagcacatcccgacgccgtgtgtatgttgtggagagcaaaaacatggccaaagacaaaggagaattcgcagcagcacattgctttgcaaaatcacttgtgctacagagcatcgttcccgaggaaataacgattccgacgctggctggcatcgatttcgcactcgttgcagcgacaaatgcaaccattgtaatagaccgagacaattggaagcattacaaggccattgtgctcgacaacaaggtggatcaggacggtggcgctaaggtggtagtcggcagtgagattcccatgaaagttctggtgcgcaaatttcagggaaatctggagttcttcgatccaaacaatggtaatgttgttatactccagaatgtgttgaagggagacggagcttgttgccagatagagatcaaagtaggcgatgctgagactatgcttgtatccgatttctcggagatattctcaaagacgcatcctctggtggcaagccaagtatttgagcttggggatgtgattgcagtcacaaaacacaaatcggactatgcggatctgtgggccattctacgcatgacatag

本发明提供由所述的抗虫性的苏铁毒蛋白基因在苏铁中的同源基因，所述同源基因的核苷酸序列如下：

SEQ ID NO.2：

ATGGAGGAGGCTGACATGGAGAACCGTGGATACGAAGAACGGCACTCACAGTCACAGTCTGCTACACCGCTCCGCCGAAGGAAGAGATCCCCTTTCTTCGGAAGGTCTTCCGATGATATTCATTCATTCAGCAAGAATCCAGAAGAATTCTTGACTAAAAACACTCTCAGCTGCAGTTTATTAACTCGCACCGCTCAGACCGTCACAAAAAGCCCAGTATTGCCCGCCCAAGGTTGGGTCAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGCTCTCTCGGGCTGCTCCGTTGTTGTGACACAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCACGGACACGGGAGAGATACGGGACAAGCGGTCGTCTGTATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGAACCCCGTCCACATATGGAAGACGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAATTGAAGCACTTCGCCAACACGCTTGAAGTGGACCCGGAGATAGTGTCCAACGCTGTCGACGGCGAATACGAAGGCGGCCAGTTTTCCAGGAACGATGATGCAATTCGTGGATGGAACGAATTACGTGCAAGCATGAGACAGAAAATGGCTGAAAGGAAGAAACGGTTGGAAAGAAACAAAGGTATGCTAGACTCATTCAACAACCCGGATCACCCCCTCTATAAATTTCGCAAACAAGTGCTCGACGAGCTCAAGGAAATGAGTGCATACGACAACACCTTGGAGGCATCCGAGATTGCGGACCGTACTTGGCTGTGGTTGGAGATCAAAAAAGCCAAAGGTACGAATGCGGTGGTAAACAGTGGAGCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGGTATGAATCTTATCAAACCCTTCGCGTGCCGGGTTACAATGAAGATATGACATCCATTGCAATGAAGAAGTTGTTCGTTGAGAGCAGTCTGACTGACGAGCAGCTGGGCGCCTTGTGCAAGCGTATATCCATCGCTAGCGATCAAGAGAACCATCTTCTGACACAGAAGACGCAGGAGATTGAGAGCACTTTCAAACAATTTTCAGATGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTGCACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGTTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATTGCCGGTTTCTCCGACATAACTCGTTTCTCGGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGCGGATGCGCATGTTGACGGAGGGATCACGGTGAAACAATTAAGCGAGGGCGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGACAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACAGGTGGCAAGATGCTGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGTTGGAGAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGACTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTAGACGTATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCTGTCCAAATGATTATAGATTGGTTCAACCGGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAATCTTGTAAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGAGGCATCGCTGTTAACATTTGGGAAGGCGTTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATCGGCACACAACTAGCCTTTGATTCCGCAAGCTTTCTAGCGGGTATTGGTGCAATTGTTGCTGGAGCATCGGGCGTGGGCATGATTTTGGGCGGAGCTGGCGTGGTATTGGGAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGGAAATCACAGCAGATGCCCAACTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACCGCGGTGGCGGATACAGATACGAATCTAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACATCTCCAAGTTCATTTCGATAGCCAATTCATATATCGGACCCATCATGGCCTCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGCTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATATTCGAGAGCAAATTGGATACAAGAACGATCATGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCAATACCAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATCAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATTAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTATATGTCTTAACCAGGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCAAATAAAACATTATGGATACTTGATGCCCGGCAACTCATTGCAGGCAACGATTCAATCACGGTCTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTACAGATCGTCATGAGTCGGGAGGAGCTTCTCCAAGTGGACTTGGCGTCTGGAGAAGCCCGCGTGTTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACACAGGTTGCATGGCCAGTTTGTAGTGGTGAACAATTATTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCACGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGTTACTGGAGGACCCTGACAGCAAAATGGCAAGAGTTGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACCCCGACAAGGGAGTAAATGGAATCATCTATCTCGTTCATCAAGAAAGCATGGTGCTTACTGCTGTCATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGTACGATTAGCCTAACAGAATTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAAATTACCCGGCCAGAAGGTTGTCGCCACCAATGCTGAGTTTGTTACTATTCTGGCTAAGGATGAGCAAGGGAGCCAAGTATGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAACCTTGACTGTTTCATCCCACAAAGTGGCTGGACAGGATGTATTCTACTTTTACAGTATCAAGGAGCAGACGGTCTATTGCCAACAAGGGACGGGCCAGACCAACGATACATCTATTGAAAGCATCCCGATTCCAGGGCTCTCTAATCTTTTAACCGTGTACGGTGAAGTATTCGCTACAACTGATGATGGTCGCATCCTTCGCCTGCTTGGGGATGGCTCAACATATCTTGACGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAGTTGCAGTATTCGGTGTTAGAGACCTACAAAATGCAGTGGTGCCAATTTGGTACCACAATGGAAAGATCGCGGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTAACAGCAACGGTGAAGCTTGGTTGTTCGACTTGGACGACGGCATTCTGTATAGCCAGCCGCTTGTGGATAGCTTTCTGTATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAATCTTTATTCCACAAGTGGAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGTTTGCATGTGACTACCATAGACGGTGTTGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAGGCCTGGCAGATATCTCGTCAAAACCGATTAGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCATCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTTGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAGCACATCCCGACGCCGTGTGTATGCTGTGGAGAGCAAACACATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGAGGAAATAACGATTCCAACGCTGGCAGGCATCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATACACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGGGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCGGAATGTGTTGAAGGGAGACGGAGCTTGTTCCCAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTTGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAG

SEQ ID NO.3：

ATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTACACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGCTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCAGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGCGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGGGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACCGGTGGCAAGATGCCGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAAAGAATGCAAGAAAACGGACATGGTTCATATCAGATTGAATTCCTCAACACCAAGAATACTGGGGAAACCCGTTGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTTGACGCATTGCGTAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGATGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCGGTCCAAATGATTATAGATTGGTTCAACCCGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAATCTTGTAAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGAGGCATCGCTGTTAACATTTGGGAAGGCGTTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATCGGCACACAACTAGCCTTTGATTCCGCAAGCTTTCTAGCGGGTATTGGTGCAATTGTTGCTGGAGCATCGGGCGTGGGCATGATTTTGGGCGGAGCTGGCGTAATATTGGGAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGCAAATCACAGCAGATGCCCAACTGGTGGGCGAGTACTTCGCACTCTTGGACGAAGCATACAGCGGTGGCGGATACAGATACGAATCCAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACGTCTCCAAGTTCATTTCGACAGTCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAACTACTTCTTCTGGGTTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATAGGGTATAAGAACGATAGTGCTACTTTGGAGCTACCGGAGGATGCCTCCGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATCAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTATATGTCTTAACCACGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCGAATAAAACATTATGGATACTTGATGCCCGGCAACTCATTGCAGGCAACGATTCAATCACGGTCTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTGCAGATCGTCATGAGTCGGGACGAGCTTCTCCAAGTGGACTTGGCGTCTCGAGAAGTCCGCGTGTTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGGAGAACACAGGTTGCATGGCCAGTTTGTAGTGGTGAACAATTATTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCACGATCGGATGTTGTATACAGATGTGGAAGATGAGACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAATCAGAATGCCCTGTGGCGAGTGAATGCAGACACACACCAAGTAATAACCAAATACTATCCGACGCTCCTGGAGGACCCTGACAGTAAAATGGCAAGAGTCGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACCCCGACAAGGGAGTAAATGGAATCATCTATCTCGTTCACCAAGAAAGTATGGTGCTCACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGCACGATTAGCTTAACAGAATTGCGAGGCGTCAATGACATGGCCCTGGTTTCCACGATGGACAGATTACCCGGCCAAAAGGTTGTCGCCACCAACGCTGAGTTTGTGACACTTCTGGCTAAGGATGAGCAAGGGAGCCAAGTCTGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAACCTTGACTGTTTCATCCCACAAAGTGGCTGGACAGGATGTATTCTACTTTTACAGTATCAAGGAGCAGACGGTCTATTGCCAACAAGGTACGGGCCAGACCAACGATACATCTATTGAAAGCATCCCGATTCCAGGGCTCTCTAATCTTTTAACCGTGTACGGTGAAGTATTCGCTACAACTAATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATATCTTGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAGTTGCAGTATTCGGTGTTAGAGACCCACAAAATGCAGTGGTGCCAATTTGGTACCACAATGGAAAGATCGCAGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTAACAGCAACGGTGAAGCTTGGTTGTTCGACTTGGACGACGGCATTCTGTATAGCCAGCCGCTTGTGGATAGCCAAGCTGTGGATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAAACCTTATTCCACAAGTGGAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGCTTGCATGTGACTACCATAGACGGTGTTGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAAGCCTGGCAGATATCTCGTCAGAACCGATTAGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCACCGGCATGGTATTATGTTCCATCAAGGGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCTATACATCTACAGCACATCTCGACGCCGTGTGTATGCTGTGGAGAGCAAAAAGATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGAGGAAATAACGATTCCAACGCTGGCTGGCACCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATAGACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTCCAAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTTGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAG

SEQ ID NO.4：

ATGGAGGAAGTTGACATGCAAAACCGTGGATACGAAGAATGGCACTCACAGTCACAGTCTGCTACAACGCTCCGCCGAAGGAAGAGATCCCCTGTCTTCGGAAGGTCTTCCGATGACATTCATTCGTTCAGCAAGAATCCAGAAGAATTCTTGACTCAAAACACTCTAAGCTGCAGTTTATTAACTCGCACCGCTGAGACCGTCACAAAAAGCCCAGTATTGCCCGAACGAGGTTGGGTTAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGCTCTCTTTGTTCAAGTGGTTGCCGAGCAATCATATGACACCGTGAAAGCGTACTTACTTGGCTACAATGGTGAGAACGACTCCCCCAACCCGGCATATGTTGATATTCCAAAGAGGGCCGCCGAGAACGGAACATTTTTGTTTACCGTTGATCTCTCGGGCTGCTCCGTTGTTGTGACGCAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCATGGACACGGGAGAGATACCGGACAAGCGGTCGTCTGTATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGAACCCCGTCAACATATGGAAGACGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAGTTGAAGAAATTCGCCGACATGTTTGGAGTGGACCCGGAGATAGTGTCCAACGCTGTCGACGGCAAATACAAGGGTGGCCAGTTTTCCAGGAACGATGATGCAATTCGTGGATGGAACGAATTACGTGCAAGCATGAGACAGAAAATGGCCGAAAGGAACAAAGTGTTGCTAACAATGAAACTGGAGCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGCTGGGCGCCTTGTGCAAGCGTATATCCATCGCGATCAAGAGAACCATCTTCTGACACAGAAGACGCAGGAGATTGAGAGCACTTTCAAACAATTTTCAGATGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAAGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTGCACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGCTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCAGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAACCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGTCATGGCGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGGGTTGATCTTACTAATGTCTTCGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACCGGTGGCAAGATGCCGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAAAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGACTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGGGACGTTCAAAGAATTAAAATCCTACCTGGATAAAAATTTAGACGCATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCAGTCCAAATGATTATAGATTGGTTCAATCGGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAGCCTTGTGAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGGGGCATCGCTGTTAACATTTGGGAAGGCGCTGAAACTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGCCTGTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATTGGCACACAACTAGACTTTGATTCCGCAAGCTTTTTAGTGAGTATCGGGGCAGCAGTTGCTGGAGTATCGGGCGTGGGCATCGTTTTGAGCGGAGCTGGCGTGTTATTGGCAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGGAAATCACAGCAGACGCCCAGCTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACCGCGGTGGCGGATACAGATACGAATCTAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACATCTCCAAGTTCATTTCGACAGCCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGTTGGCGACATGCCGAAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATTGGGTATAAGAACGATAGTGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACCACAGATTCGATTACGTTTTCTACATCTTCCCATCTGAATACACAATTAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCCGGATAGGCGCCCTTTGCGACTGCGAGCACCCGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATTGAGGGGACCGGAGGAGAATACGTTATATGTCTGAACAAGGGATCCACTGTCACACTGTTCACCACCGGGTCAGAGGCAAATAAAACATCATGGATACTCGATGCCCGGCAAATCATTGCAGGCAACGATCCAATCATAGTCTCTCAAGATAGCATCACCATGGGCAGCACAACCGTCCATCTCCGTAACCATGATTTCGATTGCCTACGGATCGTCACGAGTCGGGAGGAGCTTCTCCAAGTGGACTCGGCGTCTCGACAAACCCGCGTGCTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACATAGGTTGCTTGGCCAGTTTGTAGTGGTGAACAATTATTCCGTGCAAGAACAAAGTGACATCGGCACGCTTTATCGTGGAGTGGGCCGTGCTCATGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAGGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGGTACTGGAGGACCCTGACAGCAAAATGGCAAGAGTTGAGCAGCGTGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACTCCGACAAGGGAGTAAATGGAATCATCTATCTCGTTCATCAAGAAAGCATGGTGCTTACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAGCGAGTACGATTAGCCTAACAGAACTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAAATTACCCGGCCAGAAGGTTGTCGCCACCAATGCTGAGTTTGTCACTATTCTGGCTAAGGATGAGCAAGGGAGCCAAGTCTGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAACCTTGACTGTTTCATCCCACAAGGTGGCTGGACAGGATGTATTCTACTTTTACAGTATCAAGGAGCAGAGGGTCTATCGCCAACAAGGTACGGGCCAGACCAACGATACATCTGTTGGAAGCATCCCGATTCCAGGACTCTCTAATCTTTTCACCGTGTACGGTGAGGTATTCGCTACAACTAATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATATCTTGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAATTGCAGTATTCGGTGTTAGAGACCCACAAAATGCAGTGGTGCCAATTTGCAACGGTGAAGCGTGGTTGTTCGACTTGGACGGCGGCATTCTGTATAGCCAGCCGCTCGTGGATAGCCAAGCTGTGGATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAAACCTTATTCCACAAGTACAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTCGACGGCCGTTTGCATGTGACTACCATAGACGGTGTCGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGGGAACAAGGCCTGGCAGATATCTCGTCAGAACCGATTGGAAGAGGCCCTCAGAAATCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCATCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAGCACATCTCGATGCCGTGTGTATGCTGTGGAGAGCAAAAACATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGCGGAAATAACGATTCCAATGCTGGCCGGCATCGATTTCGCACTCGTTGCAGCGACAAAAGCAACCATTGTAATAGACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCCAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTCCCAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTCGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCGAGCCAAGTATTTGAACTTGGGGATGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAGCCTATATATCCCACGCATATCTTCACCGATGGCCTATATATGTCATTTCAACGTTGATAGCGTAG

SEQ ID NO.5：

ATGGAGGAGGCTGACATGGAGAACCGTGGAAACGAAGAACGGCACTCACAGTCACAGTCTGCTACACCGCTCCGCCGAAGGAAGAGATCCCCTGTCTTCGGAAGGTCTTCCGATGATATTCATTCATTCAGCAAGAATCCAGAAGAATTCTTGACTAGAAACACTCTCAGCTGCAGTTTATTAACTCGCACCGCTGAGGCCGTCACAAAAAGCCCAGCATTGCCCGCCCAAGGTTGGGTCAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGTTCTCTTTGTTCAGCTGGTTGCCGAGAAATCAGATGAGACAGTGAAGGCGTACTTACTTGGCTACAATGGTGAGAACGACTTCCCCAAGCCGGCATACGTTGATATTCCAAAGAGGGGCGCCAAGAACGGAACATTTTTGTTTACCGTTGATCTCTCGGGCTGCTCCGTTGTTGTGACACAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCACGGACACGGGAGAGATACGGGACAAGCGGTCGTCTGCATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGATCCCCGTCCACATATGGAAGACGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAGTTGAAGAAATTCGCCGACATGTTTGGAGTGGACCCGGAGATAGTGTCCAATGCTGTCGACGGCAAATACGAGGGCGGCCAGTTTTCCAGGAACGATGATGCAATTCGTGGATGGAACGAATTACGTGCAAGAATGAGACAGAAAATGGCTGAAAGGAAGAAACTGTTGGAAACAAAGAAAGGTATGCTAGACTCATTCAACAACCCGGATCACCCCCTCTATGAATTTCGCAAACAAGTGCTCGACGAGCTCAAGGAAATGAGTGCATACGACAACACCTTGGAGGCATCCGAGATTGCGGACCGTACTTGGCTGTGGTTGGAGATCAAAAAAGCCAGAGGTACGAATGCGGTGGTAAACAGTGGATCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGGTATGAATCTTATCAAACCGTTCGCGTGCCGGGTTACAATGAAGATATGACATCCATTGCAATGAAGAAGTTGTTCGTTGAGAGCAGTCTGACTGACGAGCAGCTGGGCGCCTTGTGCAAGCGTATATCCATCGCTAGCGATCAAGAGAACCATCTTCTGAGACAGAAGACGCAGGAGATTGAGAACACTTTCAAACAATTCTCAGACGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTGCACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGTTGCGGTGATTGCAGACGAACAGAGTCTGGCGCCCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCAGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCTGGAAATTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGCGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGGGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACCGGTGGCAAGATGCCGTCATACGATTGAGACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAAAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGACTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTAGACGCATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTAGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCAGTCCAAATGATTATAGATTGGTTCAATCGGGGGACTAATGCCGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCACTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAGCCTTGTGAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGGGGCATCGCTGTTAACATTTGGGAAGGCGCTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATTGGCACACAACTAGCCTTTGATTCCGCAAGCTTTTTAGTGAGTATCGGGGCAGCAGTTGCTGGAGTATCGGGCGTGGGCATCGTTTTGAGCGGAGCTGGCGTGTTATTGGCAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGCAAATCACAGCAGATGCCCAACTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACAGCGGTGGCGGATACAGATACGAATCCAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTAGCCGACAAACATCTCCAAGTTCATTTCGACAGCCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGCTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATCGGGTATAAGAACGATAGTGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATCAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTGTATGTCTTAACCACGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCAAATAAAACATTATGGATACTTGATGCCCGGCAACTCATTGCAGGCAACGATTCAATCACGGTTTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTACAGATCGTCATGAGTCGGGAGGAGCTTCTCCAAGTGGACTTGGCGTCTCGACAAGCCCGCGTGCTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACATAGGTTGCTTGGCCAGTTTGTAGTGGTGAACAATTATTCCGTGCAAGAGCAAAGTGACATCGGCACGCTTTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCATGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGGTACTGGAGGACCCTGACAGCAAAATGGCAAGAGTTGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCAGCCCGACAAGGGAGTAGATGGAATCATCTATCTCGTTCATCAAGAAAGCATGGTGCTTACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGTACGATTAGCTTAACAGAATTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAAATTACCCGGCCAGAAGGTTGTCGCCACCAATGCTGAGTTTGTGACTATTCTGGCTAAGGATGAGCAAGGGAGCCAAGTCTGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAGCCTTGACTGTTTCATCCCACAAGGTGGCTGGACAGGATGTATTCTACTTTTGCAGTATCAAGGAGCAGACGGTCTATCGCCAACAAGGTACGGGCCAGACCAACGATACATCTGTTGGAAGCATCCCGATTCAAGGGCTCTCTAATCTTTTCACCGTGTACGGTGAGGTATTCGCTACAACTGATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATATCTTGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGACAGCCACGACAATTGCAGTATTCGGTGTTAGAGACCCACAAAATGCAGTGGTGCCAATTTGGTACCACAGTGGAAAGATCGCGGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTCACAGCAACGATCAAGCTTGGCTGTTCGACTTGGACGGCGGCATTCTGTATAGCCAGCCGCTCGTGGATCGCCAAACTGTTGATACAATCTTTAGTTTGGACGGGAAGCTTATTGCGCCAGACCTTATTCCACAAGTACAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGTTTGCATGTGACTACCATAGACGGTGTCGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAGGCCTGGCAGATATCTCGTCAGAACCGATTGGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCGTCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAACACATCCCCTCGACGCCGTAGCATCGTTCCCGAGGAAATAACGATTCCAACGCTGGCTGGCATCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATAGACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTACTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTGCCAGATAGACATCAAAGTAGGCGATGCTGAGACTATGCTTGCATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGAGGATCTGTGGGCCATTCTACACATGACATAG

本发明提供由所述的抗虫性的苏铁毒蛋白基因优化得到的苏铁毒蛋白优化基因，所述苏铁毒蛋白优化基因的核苷酸序列如下：

SEQ ID NO.6：

atggtgatggttattgattacccttctttgagggcttctttgtatgctactaagtctgctgttccttgtaggcctcttcctggtaaccctgaaagggtttctaggagggtttctagaaggggtgctggtgaaattggtagtcatgctgatttttggatttctagagctaaggcttctgctattaggatttttcctagacaagaaggtccattgagggaaagcaggaggaggttctatttgttgagagaggctgttttcacaggtaggaggactgcatgggctgcaaatcctgctgctcagagagcagcttgggatagaaagaggtctcctttcttcggtaggtcatcagatgatattcattcattcagtaagaacccagaagagttccttactcaaaatactttgtcttgttctcttcttactagaactgctcaaactgttactaagtctcctgttttgcctgctcaaggttgggttaaggttggtagagtgccaggtagaggtgatgctcttttcgttgagcttgttgctgaaaaatctgatgaaactgttaaggcttacttgttgggttataatggtaagaatgattctcctgaacctgcttatgttgatattccaaagaggggtgcagagaatggaactttcctttttacagtggatcttagcggttgttctgttgttgttactcaattggatgagcagacttatagagtgttccatgataagagggttaattcaagtattctttacgataacgtggttatgtatgttgattttaaccattacaggtaccctagagctcatggtcatggtagagatactggtcaagctgttgtttgtatgcagtttgctgatggtgtttggaagttgttcttgcaaagacatgagactgctaattctcttccaaagatttggtctagaggtgatggtaagcctgaaattttctctgatgctgagcctagacctcatatggaggatggtattaaggagttccttaatagaaggagggctttgcaagatgaattgaagcattttgctaatactttggaagttgatcctgatattgtttctaatgctgttgatggtgaatatgaaggtggtcaattttctagaaatgatgatgcaattaggggttggaatgaattgagagcttctatgagacagaagatggctgaaagaaagaagcttcttcaaactaagaagggtatgttggatagtttcaataatccagatcatcctctttacaagttcagaaaacaagttttggatgaattgaaagagatgtcagcttatgataatacacttgaagcttctgaaatcgctgataggacttggctttggcttgaaattaagaaggctaagggaacaaatgcagttgttaatagcggagctgcacctaagcaaccatcccttactgttggtgaaagatatgctgaaatggaagcagcactttggatgagaagcgatacagctgctttcgaacaaggatatgaaagttatcaaactgttagagttccaggttataatgaagatatgacaagcattgctatgaagaagttgtttgttgaatcttccttgacagatgaacaacttggtgctctttgtaagaggatttccattgctagtgatcaagaaaatcatttgttgactcaaaagacccaagaaatcgaatcaactttcaagcaattctccgatggaagcttccttgaatctgctccacaagatttcttccttagaagagttggtgataaatccggtggtaggtgctacccacttgttagggttatggctgttgctctttctgaaggtgaagatgttgctcaaaatttcatggaaaatttgaggactttgtcaaggaatcctgaatattccggtgaatcagcttcttctcaagctacaaatgctggtcttttcagagatgctcttgctggtcttcatgcaaacgttgaagctatcgctgcatccgagaagcttcctgctgtttttggtttgcctgatgttgctactatgctttctcaggcttctgaatctaaggtttatgctcttaatactgaaactcattctatgcttgttgctgttattgttgataagcaatctttggctaggaagtattatttttacgatcctaatttccttatggctggtttctctgatattactagattctccgaagctcttaagtaccatttcgttaatttgcagtttgcaggtaattataaggctttcggtactgctgaagctcctcaattcaatcttgttcttattgatactaccgctatggaggatgctcatgttgatggtggtattactgtgaagcaactttctgaaggagttgatcttactaatgttttcgattctactaagaaggctagagtggttactgaggctgcagctaggttggctgaagatcagcaattgagaactggacttacaattattgaagctgatcagtgggctaatagatggcaggatgctgttattaggttgaagcttaaggaaggtctttctggtgagtggatgcctttgcttgaaaagatgcaagaaaatgcagatggtagttatcagattgatttcattaacactaagaacactggagaaattaggggagtttcaactagggatgctacttttaaggaattcaagtcttatttggataagaaccttgatgtgttgcataaggattttattttccaggatggagtgttcaaacctcttgagggtgagcctattgatggacttaatgctatgttcgctgttcaaatgattattgattggttcaataggggtactaacgcagaagaagatgctgcttctggttctactaacgctcaattgtctttggctattaaggttcattcttatatcaacatggcacaagtggcacatactgctgttactgatgtggcaaaggtttataatttggtgaaatctgctctttacggtgaacaagttgctggtgaaacatcattgttcactttcggtaaggctcttaaatttgctgctactgaaggtcttggtgtgttgcttggtgcttcttttgtgatccttgatgcttatcagctttctcttgttgagaatgatgttcagaaggcaattattggtactcaacttgcttttgattctgcttcttttcttgctggtattggtgctattgttgctggtgcttctggtgttggtatgattttgggtggtgctggtgttgttttgggtggtttgggtgttggttttggtgctttggctcaggcttttggtgagattactgctgatgctcagttggttggtaagtattttgctttgttggatgaggcttataggggtggtggttataggtatgagtctaaggataaggctttgatgcctttggctggtgctgtgattgataagattagacttgctgataagcatttgcaagttcattttgattctcaattcatctacaggactcatcatggttctactggttctggtaagattaattatttcttctgggctggtgatatgcctcaaatggttcatgatagatctcaagctattaatgttagggagcagattggttataagaatgataatgctactcttgaacttccagaggatgcttctgatcttattcttcctgcaactcctaagtctttcattacatatagatacgagatcttgccattcgctacttctagacatgatactggttttgatgttattagaagacttgaggaggattatagattcgattatgatttctacatcttcccttctgaatataccattagaactattaagcacgaatacgttgaaaccaaggttgctgttgtgcttgatagaagatctttgagacttggtgctcctgagcttttgaaggaacaacatggttatcttatttacgagattgaaggtacaggtggtcaatatgttatttgtttgaatcagggttctactgtgactcttaagacaactggttctgaagcaaataagactctttggattttggatgctaggcaacttattgctggtaatgatagtatcactgtttctcaagattctattactatcggttctactactgtgcatcttaggaatcaggatttcgattgtcttcagattgttatgtctagagaagaacttcttcaagttgatttggcttctggtgaagctagagttttctccgaagatgcttctaagtggcttgatacaaaggaaactattcatgaacaccttcacaagttggctgaagaacataggcttcatggtcaattcgttgttgttaataattactacaggggtgttggtagagcttattatgatgctgctcatgatagaatgctttatactgatgttgaggatgaaactcttacaagagatgctgttcttgctggtgttactggtgaagatgcttatttttataacgttaagcagaacgctttgtggagagttaacgctggtactcaccaagttattactaagtattatcctactcttcttgaggatcctgattctaacatggctagagttgagcaacatggtaatatcgttgttatggctatgtcaagattgaatcatcctgataagggtgttaacggtattatttatcttgttcatcaggaatctatcgttcttactgctgttattggaaactctgaaatgcttaagagacttagacaaacttctactatctctcttactgaacttaggggtgttaacgatatggctcttgtttcaactatggataggcttcctggtcagaaggtggtggctacaaatgcagaattcgttacacttttggctaaggatgaacaaggagctaatattaaggaacaaactgtttattgccagcaaggtactggtcaaactaatgatacttctattgaatcaatccctatccctggtttgtctaatcttcttacagtttatggtgaggttttcgctacaactaatgatggtagaatccttagattgcttggtgatggaagcacttgtcttgagggtatcaatgaacattggttgattaagcatcctaaatggtggcaggatcttgctactcttgaggcagagaaggctaccactgttgctgtttttggagttagggatttgcaaaatgctgttgttcctatttggtatcataacggtaagattgcagttgcttcatctaaactttatggtaagcaattgcagtttttgggttttaattctaacggagaagcttggcttttcgatcttgatgatggtattttgtattctcaacctttggttgattctttcctttatgctattttcagtttggatggtaagttgattgctcctatctttattcctcaggttgaacaaatgttgtccggtcaacatcttaggaaccctgttattgttgatggtaggcttcatgttactactattgatggtgttgttgttggtttcgataagaagtctcctactcttgtggctgttaataaggcttggcaaatttctagacaaaataggcttgaggaggctttgggtaatttgttgtctaattggtctgatcataacgatgctattgttcttcagggtgatcctgagtctgctcctgcttggtattatgttccttctagggagattgtgactgctaatggtttgacttgggctgatcaccctctttggcttggaatggctgttggtgctgctactccttatatttattctacttctagaaggagggtttatgttgttgagtctaagaatatggctaaggataagggtgaatttgctgctgctcattgttttgctaagtctttggttcttcagtctattgttcctgaggaaattactattccaactcttgctggtatcgatttcgctttggttgctgctactaatgctactattgttattgatagggataactggaagcattataaggcaattgttcttgataacaaggttgatcaagatggaggtgctaaggttgtggttggttctgagattcctatgaaggttcttgtgaggaagtttcaaggtaatttggaatttttcgatcctaataacggtaatgtggttattcttcaaaacgttcttaagggagatggtgcttgttgtcagattgagattaaggttggtgatgcagagactatgttggtttctgatttctctgagattttctctaagactcatcctttggttgcttctcaggttttcgaacttggtgatgttattgctgttactaagcataagagtgattatgctgatctttgggctattttgaggatgacttga

SEQ ID NO.7：

ATGGAAGAAGCTGATATGGAAAATAGAGGATACGAAGAAAGACATAGCCAAAGCCAAAGCGCTACTCCTCTTAGAAGAAGAAAGAGAAGCCCTTTCTTCGGAAGAAGCAGCGATGATATTCATAGCTTCTCAAAGAATCCTGAAGAATTCCTTACAAAAAATACTCTTTCTTGTTCTCTTCTTACTAGAACTGCTCAAACTGTTACTAAGTCTCCTGTTCTTCCTGCTCAAGGTTGGGTTAAGGTTGGTAGAGTTCCTGGTAGAGGTGATGCTCTTTCTGGTTGTTCTGTTGTTGTTACTCAACTTGATGAACAAACTTATAGAGTTTTCCATGATAAGAGAGTTAATTCTTCTATTCTTTATGATAATGTTGTTATGTATGTTGATTTCAATCATTATAGATATCCTAGAGCTCATGGTCATGGTAGAGATACTGGTCAAGCTGTTGTTTGTATGCAATTCGCTGATGGTGTTTGGAAGCTTTTCCTTCAAAGACATGAAACTGCTAATTCTCTTCCTAAGATTTGGTCTAGAGGTGATGGTAAGCCTGAAATTTTCTCTGATGCTGAACCTAGACCTCATATGGAAGATGGTATTAAGGAATTCCTTAATAGAAGAAGAGCTCTTCAAGATGAACTTAAGCATTTCGCTAATACTCTTGAAGTTGATCCTGAAATTGTTTCTAATGCTGTTGATGGTGAATATGAAGGTGGTCAATTCTCTAGAAATGATGATGCTATTAGAGGTTGGAATGAACTTAGAGCTTCTATGAGACAAAAGATGGCTGAAAGAAAGAAGAGACTTGAAAGAAATAAGGGTATGCTTGATTCTTTCAATAATCCTGATCATCCTCTTTATAAGTTCAGAAAGCAAGTTCTTGATGAACTTAAGGAAATGTCTGCTTATGATAATACTCTTGAAGCTTCTGAAATTGCTGATAGAACTTGGCTTTGGCTTGAAATTAAGAAGGCTAAGGGTACTAATGCTGTTGTTAATTCTGGTGCTGCTCCTAAGCAACCTTCTCTTACTGTTGGTGAAAGATATGCTGAAATGGAAGCTGCTCTTTGGATGAGATCTGATACTGCTGCTTTCGAACAAGGTTATGAATCTTATCAAACTCTTAGAGTTCCTGGTTATAATGAAGATATGACTTCTATTGCTATGAAGAAGCTTTTCGTTGAATCTTCTCTTACTGATGAACAACTTGGTGCTCTTTGTAAGAGAATTTCTATTGCTTCTGATCAAGAAAATCATCTTCTTACTCAAAAGACTCAAGAAATTGAATCTACTTTCAAGCAATTCTCTGATGGTTCTTTCCTTGAATCTGCTCCTCAAGATTTCTTCCTTAGAAGAGTTGGTGATAAGTCTGGTGGTAGATGTTATCCTCTTGTTAGAGTTATGGCTGTTGCTCTTTCTCAAGGTGAAGATGTTGCTCAAAATTTCATGGAAAATCTTAGAACTCTTTCTAGAAATCCTGAATATTCTGGTGAATCTGCTTCTTCTCAAGCTACTAATGCTGGTCTTTTCAGAGATGCTCTTGCTGGTCTTCATGCTAATGTTGAAGCTATTGCTGCTTCTGAAAAGCTTCCTGCTGTTTTCGGTCTTCCTGATGTTGCTACTATGCTTTCTCAAGCTTCTGAATCTAAGGTTTATGCTCTTAATACTGAAACTCATTCTATGCTTGTTGCTGTTATTGTTGATAAGCAATCTCTTGCTAGAAAGTATTATTTCTATGATCCTAATTTCCTTATTGCTGGTTTCTCTGATATTACTAGATTCTCTGAAGCTCTTAAGTATCATTTCGTTAATCTTCAATTCGCTGGTAATTATAAGGCTTTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACTGCTATGGCTGATGCTCATGTTGATGGTGGTATTACTGTTAAGCAACTTTCTGAAGGTGTTGATCTTACTAATGTTTTCGATTCTACTAAGAAGGCTAGAGTTGTTACTGAAACTGCTGCTAGACTTGCTGAAGATCAACAACTTAGAACTGGTCTTACTATTATTGAAGCTGATCAATGGGCTAATAGATGGCAAGATGCTGTTATTAGACTTAAGCTTAAGGAAGGTCTTTCTGGTGAATGGATGCCTCTTCTTGAAAAGATGCAAGAAAATGCTGATGGTTCTTATCAAATTGATTTCATTAATACTAAGAATACTGGTGAAATTAGAGGTGTTTCTACTAGAGATGCTACTTTCAAGGAATTCAAGTCTTATCTTGATAAGAATCTTGATGTTCTTCATAAGGATTTCATTTTCCAAGATGGTGTTTTCAAGCCTCTTGAAGGTGAACCTATTGATGGTCTTAATGCTATGTTCGCTGTTCAAATGATTATTGATTGGTTCAATAGAGGTACTAATGCTGAAGAAGATGCTGCTTCTGGTTCTACTAATGCTCAACTTTCTCTTGCTATTAAGGTTCATTCTTATATTAATATGGCTCAAGTTGCTCATACTGCTGTTACTGATGTTGCTAAGGTTTATAATCTTGTTAAGTCTGCTCTTTATGGTGAACAAGTTGCTGGTGAAGCTTCTCTTCTTACTTTCGGTAAGGCTCTTAAGTTCGCTGCTACTGAAGGTCTTGGTGTTCTTCTTGGTGCTTCTTTCGTTATTCTTGATGCTTATCAACTTTCTCTTGTTGAAAATGATGTTCAAAAGGCTATTATTGGTACTCAACTTGCTTTCGATTCTGCTTCTTTCCTTGCTGGTATTGGTGCTATTGTTGCTGGTGCTTCTGGTGTTGGTATGATTCTTGGTGGTGCTGGTGTTGTTCTTGGTGGTCTTGGTGTTGGTTTCGGTGCTCTTGCTCAAGCTTTCGGTGAAATTACTGCTGATGCTCAACTTGTTGGTAAGTATTTCGCTCTTCTTGATGAAGCTTATAGAGGTGGTGGTTATAGATATGAATCTAAGGATAAGGCTCTTATGCCTCTTGCTGGTGCTGTTATTGATAAGATTAGACTTGCTGATAAGCATCTTCAAGTTCATTTCGATTCTCAATTCATTTATAGAACTCATCATGGTCTTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGCTGGTGATATGCCTCAAATGGTTCATGATAGATCTCAAGCTATTAATATTAGAGAACAAATTGGTTATAAGAATGATCATGCTACTCTTGAACTTCCTGAAGATGCTTCTGATCTTATTCTTCCTGCTACTCCTAAGTCTTTCATTACTTATCAATATCAAATTCTTCCTTTCGCTACTTCTAGACATGATAGAGGTTTCGATGTTATTAGAAGACTTGAAGAAGATTATAGATTCGATTATGATTTCTATATTTTCCCTTCTGAATATACTATTAGAACTATTAAGCATGAATATGTTGAAACTAAGGTTGCTGTTGTTCTTGATAGAAGATCTCTTAGACTTGGTGCTCCTGAACTTCTTAAGGAACAACATGGTTATCTTATTTATGAAATTGAAGGTACTGGTGGTCAATATGTTATTTGTCTTAATCAAGGTTCTACTGTTACTCTTAAGACTACTGGTTCTGAAGCTAATAAGACTCTTTGGATTCTTGATGCTAGACAACTTATTGCTGGTAATGATTCTATTACTGTTTCTCAAGATTCTATTACTATTGGTTCTACTACTGTTCATCTTAGAAATCAAGATTTCGATTGTCTTCAAATTGTTATGTCTAGAGAAGAACTTCTTCAAGTTGATCTTGCTTCTGGTGAAGCTAGAGTTTTCTCTGAAGATGCTTCTAAGTGGCTTGATACTAAGGAAACTATTCATGAACATCTTCATAAGCTTGCTGAAGAACATAGACTTCATGGTCAATTCGTTGTTGTTAATAATTATTATAGAGGTGTTGGTAGAGCTTATTATGATGCTGCTCATGATAGAATGCTTTATACTGATGTTGAAGATGAAACTCTTACTAGAGATGCTGTTCTTGCTGGTGTTACTGGTGAAGATGCTTATTTCTATAATGTTAAGCAAAATGCTCTTTGGAGAGTTAATGCTGGTACTCATCAAGTTATTACTAAGTATTATCCTACTCTTCTTGAAGATCCTGATTCTAAGATGGCTAGAGTTGAACAACATGGTAATATTGTTGTTATGGCTATGTCTAGACTTAATCATCCTGATAAGGGTGTTAATGGTATTATTTATCTTGTTCATCAAGAATCTATGGTTCTTACTGCTGTTATTGGTAATTCTGAAATGCTTAAGAGACTTAGACAAACTTCTACTATTTCTCTTACTGAACTTAGAGGTGTTAATGATATGGCTCTTGTTTCTACTATGGATAAGCTTCCTGGTCAAAAGGTTGTTGCTACTAATGCTGAATTCGTTACTATTCTTGCTAAGGATGAACAAGGTTCTCAAGTTTGTTTCTGGCTTAGAACTGTTGATGGTGTTCTTATTAAGCCTTCTATTCAACCTCCTATTCCTACTGATCTTACTCTTACTGTTTCTTCTCATAAGGTTGCTGGTCAAGATGTTTTCTATTTCTATTCTATTAAGGAACAAACTGTTTATTGTCAACAAGGTACTGGTCAAACTAATGATACTTCTATTGAATCTATTCCTATTCCTGGTCTTTCTAATCTTCTTACTGTTTATGGTGAAGTTTTCGCTACTACTGATGATGGTAGAATTCTTAGACTTCTTGGTGATGGTTCTACTTATCTTGATGGTATTAATGAACATTGGCTTATTAAGCATCCTAAGTGGTGGCAAGATCTTGCTACTCTTGAAGCTGAAAAGGCTACTACTGTTGCTGTTTTCGGTGTTAGAGATCTTCAAAATGCTGTTGTTCCTATTTGGTATCATAATGGTAAGATTGCTGTTGCTTCTTCTAAGCTTTATGGTAAGCAACTTCAATTCCTTGGTTTCAATTCTAATGGTGAAGCTTGGCTTTTCGATCTTGATGATGGTATTCTTTATTCTCAACCTCTTGTTGATTCTTTCCTTTATGCTATTTTCTCTCTTGATGGTAAGCTTATTGCTCCTATTTTCATTCCTCAAGTTGAACAAATGCTTTCTGGTCAACATCTTAGAAATCCTGTTATTGTTGATGGTAGACTTCATGTTACTACTATTGATGGTGTTGTTGTTGGTTTCGATAAGAAGTCTCCTACTCTTGTTGCTGTTAATAAGGCTTGGCAAATTTCTAGACAAAATAGACTTGAAGAAGCTCTTGGTAATCTTCTTTCTAATTGGTCTGATCATAATGATGCTATTGTTCTTCAAGGTGATCCTGAATCTGCTCCTGCTTGGTATTATGTTCCTTCTAGAGAAATTGTTACTGCTAATGGTCTTACTTGGGCTGATCATCCTCTTTGGCTTGGTATGGCTGTTGGTGCTGCTACTCCTTATATTTATTCTACTTCTAGAAGAAGAGTTTATGCTGTTGAATCTAAGCATATGGCTAAGGATAAGGGTGAATTCGCTGCTGCTCATTGTTTCGCTAAGTCTCTTGTTCTTCAATCTATTGTTCCTGAAGAAATTACTATTCCTACTCTTGCTGGTATTGATTTCGCTCTTGTTGCTGCTACTAATGCTACTATTGTTATTCATAGAGATAATTGGAAGCATTATAAGGCTATTGTTCTTGATAATAAGGTTGATCAAGATGGTGGTGCTAAGGTTGTTGTTGGTTCTGGTATTCCTATGAAGGTTCTTGTTAGAAAGTTCCAAGGTAATCTTGAATTCTTCGATCCTAATAATGGTAATGTTGTTATTCTTAGAAATGTTCTTAAGGGTGATGGTGCTTGTTCTCAAATTGAAATTAAGGTTGGTGATGCTGAAACTATGCTTGTTTCTGATTTCTCTGAAATTTTCTCTAAGACTCATCCTCTTGTTGCTTCTCAAGTTTTCGAACTTGGTGATGTTATTGCTGTTACTAAGCATAAGTCTGATTATGCTGATCTTTGGGCTATTCTTAGAATGACTTGA

SEQ ID NO.8：

ATGGCAGTTGCTTTGAGCCAAGGTGAAGATGTTGCTCAGAATTTCATGGAGAATTTGAGGACTTTGAGCAGGAATCCTGAATATAGCGGTGAAAGCGCTTCCTCTCAGGCTACCAATGCTGGTCTTTTTAGGGATGCTTTGGCTGGTCTTCATGCTAACGTTGAAGCTATCGCTGCTTCTGAGAAGTTGCCTGCTGTGTTCGGATTGCCTGATGTTGCTACTATGCTTTCTCAAGCTTCTGAGTCTAAGGTTTATGCTCTTAATACTGAAACTCATTCTATGCTTGCTGCTGTTATCGTTGATAAGCAATCTTTGGCTAGGAAGTATTATTTCTATGATCCTAATTTCCTTATGGCTGGTTTCTCTGATATTACTAGATTCTCCGAAGCTCTTAAGTATCATTTTGTTAATCTTCAGTTCGCTGGTAATTATAAAGCTTTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACCGCTATGGCTGATGCTCATGTTGATGGTGGTATTACTGTGAAACAACTTTCTGAGGGTGTGGATTTGACTAATGTTTTTGATTCTACTAAGAAGGCTAGAGTGGTTACCGAGGCAGCAGCTAGGTTGGCTGAAGATCAGCAATTGAGAACTGGATTGACTATTATTGAAGCAGATCAATGGGCTAATAGATGGCAGGATGCTGTGATTAGATTGAAGCTTAAGGAAGGACTTTCTGGTGAGTGGATGCCTCTTCTTGAAAGAATGCAAGAAAATGGACACGGTAGCTATCAAATTGAATTTCTTAATACCAAGAACACCGGTGAAACAAGATGGGTTAGCACAAGAGATGCTACTTTTAAGGAATTCAAGTCTTATCTTGATAAGAACTTCGATGCTTTGAGGAAGGATTTCATTTTCCAAGATGGAGTTTTCAAACCTCTTGATGGTGAGCCTATTGATGGTCTTAATGCTATGTTCGCTGTTCAAATGATTATTGATTGGTTCAATCCTGGTACTAATGCTGAGGAGGATGCTGCTAGTGGTTCTACTAATGCTCAACTTTCTTTGGCTATTAAGGTGCATTCTTATATTAACATGGCTCAAGTTGCTCACACTGCTGTTACTGATGTTGCAAAGGTTTATAATCTTGTTAAGTCTGCTCTTTACGGTGAACAAGTTGCTGGTGAAGCTAGCTTGCTTACTTTCGGTAAGGCTCTTAAGTTCGCTGCTACTGAAGGTTTGGGTGTTCTTTTGGGTGCTTCTTTCGTTATTCTTGATGCTTATCAGCTTTCTCTTGTTGAGAATGATGTTCAGAAGGCAATTATTGGAACTCAATTGGCTTTTGATTCTGCTTCTTTTTTGGCTGGTATTGGTGCTATTGTTGCTGGTGCTTCTGGTGTTGGTATGATTTTGGGTGGTGCTGGTGTTATTTTGGGTGGATTGGGTGTTGGTTTTGGTGCTTTGGCTCAGGCTTTTGGTCAGATTACTGCTGATGCTCAGTTGGTTGGTGAGTATTTTGCTTTGTTGGATGAGGCTTATTCTGGTGGTGGTTATAGGTATGAGTCTAAGGATAAGGCTTTGATGCCTCTTGCTGGTGCTGTGATTGATAAGATTAGGCTTGCTGATAAGAGGTTGCAAGTTCATTTTGATTCTCAATTTATCTACAGGACTCATCACGGTTCTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGTTGGTGATATGCCTCAAATGGTTCATGATAGATCTCAAGCTATTAATGTTAGGGAACAGATTGGTTATAAGAATGATTCTGCTACTTTGGAATTGCCTGAAGATGCTTCTGATTTGATTTTGCCTGCTACTCCTAAGTCTTTCATTACATATAGATACGAAATCCTTCCATTCGCTACATCTAGACATGATAGAGGTTTTGATGTTATTAGAAGATTGGAAGAAGATTACAGGTTCGATTATGATTTCTACATTTTCCCTTCCGAGTACACTATTAGAACTATTAAGCATGAATACGTGGAAACTAAGGTTGCTGTTGTTCTTGATAGAAGATCTTTGAGACTTGGTGCTCCTGAACTTTTGAAGGAGCAGCATGGTTATTTGATTTACGAGATTGAAGGAACTGGTGGTCAATATGTTATTTGTTTGAATCATGGTTCCACTGTTACTCTTAAGACTACTGGTTCCGAAGCTAATAAGACACTTTGGATTTTGGATGCTAGGCAGCTTATTGCTGGAAATGATTCTATCACTGTTTCTCAAGATTCTATTACTATCGGTTCTACTACTGTTCACCTTAGAAATCAAGATTTCGATTGCCTTCAAATTGTGATGTCTAGGGATGAACTTCTTCAAGTGGATCTTGCTTCTAGGGAAGTTAGGGTTTTCTCCGAAGATGCTTCAAAATGGCTTGATACCAAGGAAACTATTCATGAACATCTTCACAAGTTGGCTGGTGAGCATAGACTTCATGGTCAATTCGTTGTTGTTAATAATTACTACAGGGGTGTGGGTAGAGCTTACTATGATGCTGCACATGATAGAATGCTTTATACTGATGTTGAAGATGAAACTCTTACAAGAGATGCAGTTCTTGCTGGTGTTACTGGAGAAGATGCTTATTTTTATAACGTTAACCAGAACGCTCTTTGGAGGGTTAACGCTGATACTCATCAAGTTATTACTAAGTATTACCCTACCTTGTTGGAGGATCCTGATTCTAAAATGGCTAGAGTTGAGCAACATGGAAATATCGTTGTTATGGCTATGTCAAGATTGAATCATCCTGATAAGGGTGTTAACGGTATTATTTATCTTGTTCATCAGGAATCTATGGTTCTTACTGCTGTTATTGGTAATTCTGAAATGCTTAAGAGGTTGAGACAAACTTCTACTATCTCTCTTACTGAACTTAGGGGTGTTAACGATATGGCTCTTGTTTCTACTATGGATAGACTTCCTGGTCAAAAGGTGGTTGCTACTAATGCTGAATTCGTGACACTTCTTGCTAAGGATGAACAAGGTTCTCAAGTGTGTTTTTGGTTGAGGACTGTTGATGGTGTTTTGATTAAGCCTTCTATTCAGCCTCCTATTCCTACTGATCTTACTCTTACTGTTTCTTCTCATAAGGTTGCTGGTCAGGATGTTTTTTATTTTTATTCTATCAAGGAGCAGACCGTTTATTGTCAACAAGGTACCGGACAAACCAACGATACCTCTATTGAATCTATTCCTATTCCTGGTTTGTCTAATCTTCTTACTGTTTATGGTGAGGTTTTTGCTACTACTAATGATGGTAGAATTCTTAGACTTTTGGGTGATGGAAGCACTTATCTTGAAGGTATTAATGAGCATTGGTTGATTAAGCATCCTAAGTGGTGGCAGGATTTGGCTACTTTGGAGGCAGAGAAGGCTACAACCGTTGCAGTTTTTGGTGTGAGGGATCCTCAAAATGCTGTGGTGCCTATTTGGTATCATAACGGAAAAATTGCTGTTGCTTCAAGCAAGCTTTATGGTAAGCAATTGCAATTCCTTGGATTTAATTCTAACGGTGAAGCATGGCTTTTCGATCTTGATGATGGTATTTTGTATTCTCAACCTTTGGTTGATTCTCAAGCTGTTGATGCTATTTTTTCTCTTGATGGTAAGCTTATTGCTCCAAATTTGATTCCTCAAGTTGAACAGATGTTGTCTGGTCAACACCTTAGGAATCCTGTTATTGTGGATGGTAGGTTGCATGTTACTACTATTGATGGTGTTGTTGTTGGTTTCGATAAGAAGTCTCCTACTCTTGTGGCTGTTAATAAGGCTTGGCAAATTTCTAGACAAAATAGGTTGGAAGAGGCTTTGGGTAATTTGTTGTCTAATTGGTCTGATCATAACGATGCTATTGTTCTTCAGGGTGATCCTGAGTCTGCTCCTGCTTGGTATTATGTTCCTTCTAGGGAGATTGTGACTGCTAATGGTTTGACTTGGGCTGATCACCCTCTTTGGCTTGGAATGGCTGTTGGTGCTGCTACTTTGTATATTTATTCTACTTCTAGGAGGAGAGTTTATGCTGTTGAGTCTAAGAAGATGGCTAAGGATAAAGGTGAATTTGCTGCTGCTCATTGTTTTGCTAAGTCTCTTGTTCTTCAATCTATTGTTCCTGAAGAAATTACAATCCCAACTTTGGCTGGTACTGATTTCGCTTTGGTTGCTGCTACTAATGCAACTATTGTTATTGATAGAGATAACTGGAAGCATTATAAGGCTATTGTGTTGGATAATAAGGTTGATCAAGATGGAGGTGCTAAGGTTGTGGTTGGTTCTGAGATTCCTATGAAGGTTTTGGTTAGAAAGTTTCAAGGTAACTTGGAATTCTTCGATCCTAATAATGGTAATGTTGTTATCCTTCAAAACGTTCTTAAGGGAGATGGTGCTTGTTCTAAGATTGAAATTAAAGTTGGTGATGCTGAAACTATGCTTGTTAGTGATTTCTCTGAGATTTTCTCTAAGACTCATCCTTTGGTTGCTTCTCAGGTTTTCGAACTTGGTGATGTTATTGCAGTTACTAAGCATAAGTCTGATTATGCTGATCTTTGGGCTATTTTGAGGATGACTTGA

SEQ ID NO.9：

ATGGAAGAAGTTGATATGCAAAACAGAGGATACGAAGAATGGCACAGCCAAAGCCAAAGCGCTACTACTTTGAGAAGAAGAAAGAGAAGCCCTGTGTTCGGTAGGAGCAGCGATGATATTCATAGCTTCAGCAAGAATCCAGAAGAATTCCTTACTCAAAACACACTTTCATGTTCACTTCTTACTAGGACAGCTGAGACAGTTACCAAGTCTCCTGTTCTTCCAGAAAGAGGATGGGTTAAAGTGGGTAGAGTTCCAGGTAGAGGTGATGCTCTTTTCGTTCAAGTTGTTGCTGAACAATCATATGATACTGTGAAGGCTTATTTGCTTGGTTATAATGGTGAGAATGATTCTCCTAATCCTGCTTATGTTGATATTCCTAAGAGGGCTGCTGAGAATGGTACTTTCCTTTTTACTGTTGATTTGTCTGGTTGTTCTGTTGTTGTTACTCAGTTGGATGAGCAAACTTATAGAGTGTTTCATGATAAGAGAGTTAACTCATCAATCCTTTATGATAACGTTGTTATGTACGTTGATTTCAATCATTACAGGTATCCTAGGGCTCACGGTCATGGTAGAGATACTGGTCAAGCTGTTGTTTGTATGCAATTTGCTGATGGTGTTTGGAAGCTTTTCCTTCAAAGACATGAGACTGCTAATTCTCTTCCTAAGATTTGGTCTAGAGGTGATGGTAAACCTGAAATTTTCTCTGATGCTGAGCCTAGACAACATATGGAGGATGGTATCAAGGAGTTCCTTAATAGAAGGAGAGCTTTGCAAGATGAATTGAAGAAGTTCGCAGATATGTTCGGAGTTGATCCTGAGATTGTTTCTAATGCTGTGGATGGTAAGTATAAGGGTGGTCAATTTTCTAGAAATGATGATGCAATTAGGGGTTGGAATGAATTGAGAGCTTCAATGAGGCAAAAGATGGCTGAAAGAAACAAGGTGTTGCTTACTATGAAGTTGGAACAACCTAGAAGCAGCAGGGTTTTGCCTTTGGTGAATGCTACTCAAAGATGGAGGAGGCCTTTCGGTTGTGTTGCAACTCAAAGAAGACTTTCTAAGGCTGGTAGATTGGTTCAGGCTTATATTCATAGGGATCAGGAGAATCATTTGTTGACTCAAAAGACTCAAGAGATTGAGTCCACTTTCAAGCAATTCTCAGATGGTTCATTTCTTGAATCTGCTCCACAAGATTTCTTCCTTAGGAGAGTGGGTGATAAGTCTGGTGGTAGGTGTTACCCACTTGTTAGAGTTATGGCTGTTGCTCTTTCTCAGGGTGAAGATGTTGCTCAAAATTTCATGGAAAACCTTAGGACTCTTTCAAAGAATCCTGAGTATTCTGGTGAATCTGCTTCTTCTCAAGCTACAAATGCTGGTCTTTTCAGAGATGCTCTTGCTGGTCTTCATGCTAACGTTGAAGCTATTGCTGCTTCTGAAAAGTTGCCTGCTGTGTTCGGTTTGCCTGATGTTGCTACTATGCTTTCCCAAGCTTCTGAATCTAAGGTTTATGCTCTTAATACTGAAACTCATTCTATGCTTGCTGCTGTTATTGTTGATAAGCAATCTTTGGCTAGGAAGTATTATTTTTACGATCCTAATTTCCTTATGGCTGGTTTCTCTGATATTACTAGATTCTCCGAAGCTCTTAAGTACCATTTTGTTAATCTTCAGTTCGCAGGTAATTATAAGCCATTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACCGTTATGGCTGATGCACATGTTGATGGTGGTATTACTGTTAAGCAACTTTCTGAGGGTGTGGATCTTACTAATGTTTTCGATTCTACTAAGAAGGCTAGAGTTGTTACTGAAGCAGCTGCTAGGTTGGCTGAAGATCAGCAATTGAGAACTGGACTTACCATTATTGAAGCTGATCAATGGGCTAATAGATGGCAGGATGCTGTTATTAGACTTAAGCTTAAGGAAGGACTTTCTGGTGAGTGGATGCCTTTGCTTGAAAAGATGCAAGAAAATGCAGATGGTAGTTATCAAATCGATTTCATCAACACTAAGAACACTGGTGAGATTAGAGGTGTTTCAACTAGAGATGGAACTTTCAAGGAACTTAAGTCTTATTTGGATAAGAACTTGGATGCTTTGCACAAAGATTTCATTTTTCAAGATGGTGTTTTCAAGCCTTTGGAGGGTGAGCCTATTGATGGTCTTAATGCAATGTTCGCTGTTCAAATGATTATTGATTGGTTCAATAGGGGTACTAATGCTGAAGAGGATGCTGCTTCTGGTAGTACTAACGCTCAGTTGTCTTTGGCTATTAAGGTTCATTCTTATATCAACATGGCTCAAGTTGCTCATACTGCTGTTACTGATGTTGCTAAGGTTTATTCACTTGTTAAGTCTGCTTTGTACGGTGAACAAGTTGCTGGTGGTGCTTCATTGCTTACTTTCGGTAAGGCTTTGAAGTTGGCTGCTACTGAAGGTCTTGGTGTTCTTTTGGGTGCTCCTGTTGTTATTCTTGATGCTTATCAGCTTTCTCTTGTTGAAAATGATGTTCAGAAGGCTATTATTGGTACTCAGTTGGATTTCGATTCTGCTTCTTTTCTTGTTTCTATTGGTGCTGCTGTTGCTGGTGTTTCTGGTGTTGGTATTGTTCTTTCTGGTGCTGGTGTTTTGTTGGCTGGTTTGGGTGTTGGTTTTGGTGCTTTGGCTCAGGCTTTTGGTGAGATTACTGCTGATGCTCAGTTGGTTGGTAAGTATTTTGCTTTGTTGGATGAGGCTTATAGGGGTGGTGGTTATAGGTATGAGTCTAAGGATAAGGCTTTGATGCCTTTGGCTGGTGCTGTGATTGATAAGATTAGACTTGCTGATAAGCATTTGCAAGTTCATTTTGATTCTCAATTCATCTACAGGACTCATCATGGTTCTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGTTGGTGATATGCCTAAGATGGTTCATGATAGATCTCAAGCTATTAATGTTAGGGAACAGATTGGTTATAAGAATGATTCTGCTACTTTGGAATTGCCTGAAGATGCTTCTGATTTGATTTTGCCTGCTACTCCTAAGTCTTTCATTACATATAGATACGAAATCCTTCCATTCGCTACATCTAGACATGATAGAGGTTTTGATGTTATTAGAAGATTGGAAGAAGATCATAGATTCGATTATGTTTTCTACATCTTCCCTTCCGAATATACAATTAGAACTATTAAGCACGAGTACGTTGAAACTAAGGTGGCTGTGGTTCCTGATAGAAGACCTCTTAGGTTGAGAGCACCTGAACTTCTTAAGGAACAACATGGTTATCTTATTTACGAGATTGAAGGAACAGGTGGTGAATATGTTATTTGTTTGAATAAGGGTTCCACTGTGACACTTTTTACTACTGGTTCTGAAGCTAATAAGACTTCTTGGATCCTTGATGCAAGGCAAATTATTGCTGGTAATGATCCTATTATCGTTTCTCAGGATTCTATTACTATGGGTTCTACTACCGTTCACCTTAGGAATCATGATTTCGATTGCCTTAGAATTGTTACTTCTAGAGAGGAGCTTCTTCAAGTTGATTCTGCTTCAAGACAAACTAGGGTTCTTTCTGAAGATGCTTCAAAGTGGCTTGATACTAAAGAAACTATTCATGAGCATCTTCATAAGCTTGCTGAAGAGCATAGGCTTCTTGGTCAATTCGTTGTTGTGAATAACTATTCTGTTCAAGAACAGTCTGATATTGGAACTCTTTACAGAGGTGTTGGTAGAGCACATGATAGAATGCTTTATACTGATGTTGAGGATGAAACTCTTACTAGGGATGCTGTGCTTGCTGGTGTTACTGGTGAAGATGCTTACTTTTATAATGTTAAGCAGAACGCTTTGTGGAGAGTTAATGCTGGTACTCACCAAGTTATTACTAAGTATTATCCTACAGTGCTTGAAGATCCTGATTCTAAAATGGCTAGAGTTGAGCAAAGAGGTAATATCGTTGTTATGGCTATGTCAAGACTTAATCATTCTGATAAGGGTGTTAATGGTATTATTTACCTTGTTCACCAAGAATCTATGGTTCTTACTGCTGTTATTGGAAATTCAGAAATGCTTAAGAGGCTTAGACAAGCTAGCACAATCTCCCTTACTGAACTTAGGGGAGTGAACGATATGGCTTTGGTTTCTACTATGGATAAGCTTCCTGGTCAGAAGGTTGTTGCTACTAATGCTGAGTTCGTTACAATTCTTGCAAAGGATGAACAGGGATCTCAAGTTTGTTTTTGGCTTAGAACTGTTGATGGTGTTCTTATTAAGCCTTCTATTCAACCACCTATCCCTACAGATCTTACTCTTACTGTTTCTTCTCATAAAGTTGCTGGTCAAGATGTTTTCTATTTTTATTCCATCAAGGAGCAAAGGGTTTATAGACAACAAGGTACCGGACAAACAAATGATACCTCTGTTGGATCTATTCCTATCCCTGGACTTTCTAATCTTTTCACTGTTTATGGTGAGGTTTTTGCTACAACTAATGATGGTAGAATTCTTAGACTTCTTGGTGATGGATCTACTTATCTTGAGGGTATTAACGAACATTGGCTTATTAAGCATCCTAAGTGGTGGCAAGATTTGGCTACTCTTGAAGCAGAGAAGGCTACAACCATTGCTGTTTTCGGTGTTAGGGATCCTCAAAATGCTGTGGTGCCTATTTGTAATGGTGAGGCTTGGCTTTTCGATCTTGATGGTGGTATCCTTTATTCTCAACCTTTGGTTGATTCTCAGGCTGTTGATGCTATTTTCTCTCTTGATGGTAAGCTTATTGCTCCTAATCTTATTCCTCAAGTTCAACAAATGCTTTCTGGTCAGCATCTTAGGAATCCTGTTATTGTTGATGGTAGGCTTCATGTTACTACCATTGATGGTGTTGTTGTTGGTTTCGATAAGAAGTCTCCTACTCTTGTTGCTGGTAATAAGGCTTGGCAAATTTCTAGACAAAATAGGCTTGAGGAAGCTTTGAGAAATTTGTTGTCTAATTGGTCTGATCATAACGATGCTATTGTTCTTCAAGGTGATCCTGAGTCTGCTCCAGCATGGTATTATGTTCCTTCAAGAGAGATTGTGACTGCTAATGGTTTGACTTGGGCTGATCACCCTCTTTGGCTTGGTATGGCTGTTGGTGCTGCAACTCCTTATATTTATTCTACTTCTAGATGCAGAGTGTATGCTGTGGAATCTAAGAATATGGCTAAGGATAAAGGTGAATTTGCTGCTGCTCATTGTTTCGCTAAGTCTTTGGTTCTTCAATCTATTGTTCCTGCTGAAATTACTATTCCTATGCTTGCTGGTATTGATTTCGCTTTGGTTGCTGCTACTAAGGCTACTATTGTTATTGATAGAGATAACTGGAAGCATTATAAGGCTATTGTTTTGGATAACAAGGTGGATCAAGATGGTGGTGCTAAGGTTGTTGTTGGAAGTGAGATTCCTATGAAGGTTTTGGTGAGAAAGTTTCAAGGTAATTTGGAATTTTTCGATCCTAATAACGGTAATGTTGTTATTTTGCAAAACGTTCTTAAGGGTGATGGTGCTTGTTCTCAGATTGAAATTAAAGTTGGTGATGCTGAGACTATGCTTGTGTCTGATTTCTCTGAAATTTTTTCTAAGACCCATCCTCTTGTTGCTTCTCAGGTTTTCGAATTGGGTGATGGTTGTGATTGTAGTCATAAGACTCAAATTGGTTTGTGTGGTTCTGTTGGTCATTCTACTCATGATATTGCTTATATTTCCCATGCTTATTTGCATAGATGGCCTATTTATGTTATCTCTACTTTGATTGCATGA

SEQ ID NO.10：

ATGGAAGAAGCTGATATGGAAAATAGAGGAAATGAAGAAAGACATAGCCAAAGCCAAAGCGCTACTCCTTTGAGAAGAAGAAAGAGAAGCCCTGTTTTCGGTAGAAGCAGCGATGATATTCATAGCTTCAGCAAGAATCCTGAAGAATTCCTTACTAGAAACACACTTTCATGTTCACTTTTGACCAGGACAGCTGAAGCAGTTACAAAGTCTCCAGCTCTTCCTGCACAAGGATGGGTTAAAGTTGGTAGAGTTCCTGGAAGAGGTGATGTTCTTTTCGTTCAACTTGTTGCTGAAAAGTCTGATGAAACTGTTAAAGCTTATTTGCTTGGTTATAACGGTGAGAATGATTTTCCTAAGCCTGCTTATGTTGATATTCCTAAGAGGGGTGCTAAGAATGGTACTTTTCTTTTTACAGTGGATCTTTCTGGTTGTTCTGTTGTTGTTACTCAATTGGATGAGCAAACTTATAGAGTGTTCCATGATAAGAGGGTTAATTCTTCAATTCTTTACGATAACGTGGTTATGTATGTTGATTTTAACCATTACAGGTACCCTAGGGCTCACGGTCATGGTAGAGATACTGGTCAAGCTGTTGTTTGTATGCAATTTGCTGATGGTGTTTGGAAGTTGTTCTTGCAAAGACATGAGACTGCTAATTCTCTTCCTAAGATTTGGTCTAGAGGTGATGGTAAACCTGAAATTTTCTCTGATGCTGATCCTAGACCTCATATGGAGGATGGTATTAAGGAGTTCCTTAATAGAAGGAGGGCTTTGCAAGATGAATTGAAGAAGTTCGCAGATATGTTCGGAGTTGATCCTGAAATTGTTTCTAATGCAGTGGATGGTAAGTATGAAGGTGGACAATTTTCTAGAAATGATGATGCTATTAGGGGTTGGAACGAATTGAGAGCTAGAATGAGACAGAAGATGGCTGAAAGGAAGAAGCTTCTTGAAACTAAGAAGGGAATGTTGGATAGCTTCAATAATCCAGATCATCCTCTTTATGAATTCAGGAAGCAAGTTTTGGATGAATTGAAAGAAATGAGCGCTTATGATAATACTCTTGAAGCTAGCGAGATTGCTGATAGGACTTGGTTGTGGCTTGAAATTAAGAAGGCTAGAGGAACTAACGCAGTTGTTAATAGCGGAAGCGCACCTAAGCAACCTTCCCTTACTGTTGGTGAAAGATATGCAGAGATGGAAGCAGCACTTTGGATGAGAAGCGATACAGCTGCTTTCGAACAAGGTTATGAAAGTTACCAAACTGTTAGAGTTCCTGGTTATAATGAAGATATGACTAGCATTGCTATGAAGAAGCTTTTTGTTGAAAGTAGCCTTACAGATGAACAACTTGGTGCTCTTTGTAAGAGGATTTCTATTGCTAGTGATCAAGAAAATCACCTTTTGAGACAGAAGACTCAAGAAATTGAGAATACCTTCAAGCAATTCTCCGATGGAAGCTTCCTTGAAAGTGCTCCACAAGATTTCTTCCTTAGGAGAGTTGGTGATAAGAGCGGTGGTAGGTGTTATCCTTTGGTTAGAGTTATGGCTGTTGCTCTTTCTCAAGGTGAAGATGTTGCTCAAAATTTCATGGAAAATTTGAGGACTCTTAGTAGGAATCCTGAGTATTCTGGTGAGTCAGCTTCTTCTCAGGCTACCAATGCTGGTCTTTTTAGAGATGCTCTTGCTGGTTTGCATGCTAACGTTGAAGCTATCGCTGCTTCTGAAAAGCTTCCAGCTGTTTTCGGTTTGCCTGATGTTGCTACTATGCTTTCTCAAGCTTCTGAATCTAAGGTTTATGCTCTTAATACTGAAACTCACTCTATGCTTGTTGCTGTTATTGCTGATGAACAATCTCTTGCTCCTAAGTATTATTTCTATGATCCTAATTTCCTTATGGCTGGTTTCTCAGATATTACTAGATTCTCCGAAGCTCTTAAGTATCATTTTGTTAATCTTCAGTTCGCTGGTAATTATAAGGCTTTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACCGCTATGGCTGATGCTCATGTTGATGGTGGTATTACTGTGAAACAACTTTCTGAGGGTGTTGATTTGACTAATGTTTTTGATTCTACCAAGAAGGCTAGGGTTGTTACCGAAGCAGCTGCTAGGTTGGCTGAAGATCAGCAGTTGAGAACTGGACTTACAATTATCGAAGCTGATCAGTGGGCTAACAGATGGCAGGATGCTGTTATCAGACTTAGACTTAAGGAAGGACTTTCTGGTGAGTGGATGCCTTTGCTTGAAAAGATGCAAGAAAATGCAGATGGTAGTTATCAGATTGATTTCATTAACACTAAGAACACTGGTGAAATTAGAGGTGTGTCAACTAGAGATGCTACTTTTAAGGAATTTAAGTCTTACCTTGATAAGAACCTTGATGCTTTGCATAAGGATTTCATTTTCCAGGATGGAGTGTTCAAACCTTTGGAGGGTGAGCCTATTGATGGTTTGAATGCTATGTTCGCTGTTCAGATGATTATTGATTGGTTCAATAGAGGTACTAACGCTGAGGAAGATGCTGCTTCTGGTTCTACTAATGCTCAGTTGTCTCTTGCTATTAAGGTTCATTCTTATATCAACATGGCTCAAGTGGCTCACACTGCTGTTACAGATGTTGCTAAGGTTTATTCTCTTGTTAAGTCTGCTCTTTACGGTGAACAAGTTGCTGGTGGTGCTTCTCTTCTTACTTTCGGTAAGGCTCTTAAATTCGCTGCTACTGAAGGTTTGGGTGTTTTGTTGGGTGCTTCTTTCGTTATTCTTGATGCTTATCAGCTTTCTCTTGTTGAAAATGATGTTCAAAAGGCTATTATCGGTACTCAGTTGGCTTTCGATTCTGCTTCTTTTTTGGTTTCTATTGGTGCTGCTGTTGCTGGTGTTTCTGGTGTTGGTATTGTTCTTTCTGGTGCTGGTGTTCTTTTGGCTGGTTTGGGTGTGGGTTTTGGTGCTTTGGCTCAGGCTTTTGGTCAGATTACTGCTGATGCTCAGTTGGTTGGTAAGTATTTTGCTTTGTTGGATGAGGCTTATTCTGGTGGTGGTTATAGGTATGAGTCTAAGGATAAGGCTTTGATGCCTTTGGCTGGTGCTGTGATTGATAAGATTAGACTTGCTGATAAGCATTTGCAAGTTCATTTTGATTCTCAATTCATCTACAGGACTCATCATGGTTCTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGCTGGTGATATGCCTCAAATGGTTCATGATAGATCTCAAGCTATTAATGTTAGGGAGCAGATTGGTTATAAGAATGATTCTGCTACTTTGGAATTGCCTGAAGATGCTTCTGATTTGATTTTGCCTGCTACTCCTAAGTCTTTCATTACATATAGATACGAGATCTTGCCATTTGCTACTTCTAGGCATGATAGAGGTTTTGATGTTATTAGAAGATTGGAAGAAGATTACAGGTTCGATTATGATTTCTACATTTTCCCTTCCGAATATACTATTAGGACTATTAAGCATGAGTACGTTGAAACTAAGGTGGCTGTTGTGTTGGATAGAAGATCTTTGAGATTGGGTGCTCCTGAGCTTTTGAAGGAGCAGCATGGTTATCTTATTTATGAAATTGAGGGTACTGGTGGTCAATATGTGGTTTGTTTGAATCATGGTAGTACTGTGACTTTGAAGACTACTGGTTCAGAAGCAAATAAGACACTTTGGATTTTGGATGCTAGACAGTTGATTGCTGGTAATGATAGTATTACTGTTTCTCAAGATTCCATTACTATCGGATCTACTACTGTTCACCTTAGAAATCAAGATTTCGATTGCCTTCAAATTGTTATGAGTAGAGAAGAGCTTTTGCAGGTGGATCTTGCTTCAAGACAGGCTAGGGTTTTGTCAGAAGATGCTTCAAAATGGCTTGATACAAAGGAAACTATCCATGAACACCTTCACAAGTTGGCTGAAGAACATAGACTTCTTGGTCAATTCGTGGTTGTGAATAATTATAGTGTTCAAGAACAGAGTGATATCGGTACTCTTTACAGAGGAGTTGGTAGAGCTTATTACGATGCTGCTCATGATAGAATGCTTTATACTGATGTTGAGGATGAGACTTTGACTAGGGATGCTGTTTTGGCAGGTGTGACTGGTGAGGATGCATATTTTTATAATGTGAAACAGAACGCTCTTTGGAGAGTTAATGCTGGTACTCACCAAGTTATTACTAAGTATTATCCTACAGTGTTGGAAGATCCTGATTCTAAGATGGCTAGAGTTGAACAGCATGGTAATATTGTTGTTATGGCTATGTCTAGGTTGAATCAACCTGATAAGGGTGTTGATGGAATTATTTATTTGGTGCATCAAGAATCTATGGTGTTGACTGCTGTTATTGGTAATTCTGAGATGCTTAAGAGACTTAGACAAACTTCAACTATTTCTCTTACTGAATTGAGGGGTGTGAACGATATGGCTCTTGTTTCTACTATGGATAAGCTTCCAGGTCAGAAGGTGGTTGCTACTAACGCAGAATTCGTTACAATTTTGGCTAAGGATGAACAAGGAAGCCAGGTTTGTTTTTGGCTTAGGACTGTTGATGGTGTTTTGATTAAGCCTTCCATTCAACCTCCTATTCCTACAGATCTTGCTCTTACTGTTTCTTCTCATAAAGTTGCTGGTCAAGATGTTTTTTATTTCTGTTCTATCAAGGAGCAGACTGTTTATAGACAACAAGGTACCGGACAAACCAACGATACATCTGTTGGTTCTATTCCTATCCAAGGTCTTTCTAATCTTTTCACTGTTTATGGTGAGGTTTTCGCTACTACTGATGATGGTAGGATTCTTAGATTGCTTGGTGATGGTTCTACTTATCTTGAGGGTATTAACGAACATTGGTTGATTAAGCATCCTAAGTGGTGGCAAGATTTGGCTACTCTTGAAGCAGAGACTGCTACTACTATTGCAGTTTTCGGTGTTAGGGATCCTCAAAATGCTGTTGTTCCTATTTGGTATCATTCCGGTAAGATTGCTGTTGCTTCTTCTAAGCTTTATGGTAAGCAGCTTCAATTTCTTGGTTTCCATTCTAATGATCAAGCTTGGCTTTTCGATCTTGATGGTGGTATCTTGTATTCTCAACCTCTTGTTGATAGGCAAACTGTTGATACTATTTTCTCCCTTGATGGTAAGCTTATTGCTCCTGATTTGATTCCTCAAGTTCAACAAATGCTTTCTGGTCAGCACCTTAGAAACCCTGTTATTGTTGATGGTAGACTTCATGTGACTACCATTGATGGTGTTGTTGTGGGTTTCGATAAGAAGAGCCCTACTCTTGTTGCTGTGAATAAGGCTTGGCAAATTTCCAGGCAAAATAGGTTGGAGGAGGCTCTTGGTAATTTGCTTTCTAATTGGTCTGATCATAACGATGCTATTGTTCTTCAAGGTGATCCTGAGTCTGCTCCTGCATGGTATTACGTTCCTTCTAGAGAAATTGTGACTGCTAATGGTCTTACTTGGGCTGATCATCCTTTGTGGCTTGGTATGGCTGTTGGTGCTGCTACTCCTTATATTTATAATACTTCTCCTAGGAGGAGGTCTATTGTTCCTGAAGAGATTACTATCCCTACTCTTGCTGGTATTGATTTCGCTTTGGTTGCTGCTACTAATGCTACTATTGTTATTGATAGAGATAACTGGAAGCATTATAAGGCAATTGTTCTTGATAACAAGGTTGATCAAGATGGAGGTGCTAAGGTTGTGTTGGGTTCTGAGATTCCTATGAAGGTTTTGGTTAGAAAGTTTCAAGGTAATTTGGAATTCTTCGATCCTAATAATGGTAATGTTGTGATTTTGCAAAACGTTCTTAAGGGTGATGGTGCTTGTTGTCAGATTGATATTAAAGTTGGTGATGCTGAAACTATGCTTGCTTCTGATTTCTCTGAGATTTTCTCCAAGACCCATCCTCTTGTTGCTTCTCAGGTTTTCGAATTGGGTGATGTTATTGCTGTTACTAAGCATAAGTCTGATTATGAGGATCTTTGGGCTATTTTGCATATGACTTGA

本发明提供编码所述的抗虫性的苏铁毒蛋白基因及其同源基因的蛋白质，所述蛋白质的氨基酸序列如下：

SEQ ID NO.11

Met Val Met Val Ile Asp Tyr Pro Ser Leu Arg Ala Ser Leu Tyr Ala ThrLys Ser Ala Val Pro Cys Arg Pro Leu Pro Gly Asn Pro Glu Arg Val Ser Arg ArgVal Ser Arg Arg Gly Ala Gly Glu Ile Gly Ser His Ala Asp Phe Trp Ile Ser ArgAla Lys Ala Ser Ala Ile Arg Ile Phe Pro Arg Gln Glu Gly Pro Leu Arg Glu SerArg Arg Arg Phe Tyr Leu Leu Arg Glu Ala Val Phe Thr Gly Arg Arg Thr Ala TrpAla Ala Asn Pro Ala Ala Gln Arg Ala Ala Trp Asp Arg Lys Arg Ser Pro Phe PheGly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu ThrGln Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr Ala Gln Thr Val Thr Lys SerPro Val Leu Pro Ala Gln Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly AspAla Leu Phe Val Glu Leu Val Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr LeuLeu Gly Tyr Asn Gly Lys Asn Asp Ser Pro Glu Pro Ala Tyr Val Asp Ile Pro LysArg Gly Ala Glu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys Ser ValVal Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp Lys Arg Val AsnSer Ser Ile Leu Tyr Asp Asn Val Val Met Tyr Val Asp Phe Asn His Tyr Arg TyrPro Arg Ala His Gly His Gly Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln PheAla Asp Gly Val Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu ProLys Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro ArgPro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala Leu Gln AspGlu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp Pro Asp Ile Val Ser Asn AlaVal Asp Gly Glu Tyr Glu Gly Gly Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg GlyTrp Asn Glu Leu Arg Ala Ser Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu LeuGln Thr Lys Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr LysPhe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp Asn Thr LeuGlu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu Ile Lys Lys Ala LysGly Thr Asn Ala Val Val Asn Ser Gly Ala Ala Pro Lys Gln Pro Ser Leu Thr ValGly Glu Arg Tyr Ala Glu Met Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala AlaPhe Glu Gln Gly Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu AspMet Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp Glu GlnLeu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln Glu Asn His Leu LeuThr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe Lys Gln Phe Ser Asp Gly Ser PheLeu Glu Ser Ala Pro Gln Asp Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly GlyArg Cys Tyr Pro Leu Val Arg Val Met Ala Val Ala Leu Ser Glu Gly Glu Asp ValAla Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser GlyGlu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala GlyLeu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe GlyLeu Pro Asp Val Ala Thr Met Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala LeuAsn Thr Glu Thr His Ser Met Leu Val Ala Val Ile Val Asp Lys Gln Ser Leu AlaArg Lys Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile ThrArg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn TyrLys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr ThrAla Met Glu Asp Ala His Val Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu GlyVal Asp Leu Thr Asn Val Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu AlaAla Ala Arg Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu AlaAsp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu GlyLeu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu Asn Ala Asp Gly SerTyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr Gly Glu Ile Arg Gly Val Ser ThrArg Asp Ala Thr Phe Lys Glu Phe Lys Ser Tyr Leu Asp Lys Asn Leu Asp Val LeuHis Lys Asp Phe Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro IleAsp Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg GlyThr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu AlaIle Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala His Thr Ala Val Thr AspVal Ala Lys Val Tyr Asn Leu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala GlyGlu Thr Ser Leu Phe Thr Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly LeuGly Val Leu Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu ValGlu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala SerPhe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly Val Gly Met Ile LeuGly Gly Ala Gly Val Val Leu Gly Gly Leu Gly Val Gly Phe Gly Ala Leu Ala GlnAla Phe Gly Glu Ile Thr Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu LeuAsp Glu Ala Tyr Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu MetPro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln ValHis Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly LysIle Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro Gln Met Val His Asp Arg Ser GlnAla Ile Asn Val Arg Glu Gln Ile Gly Tyr Lys Asn Asp Asn Ala Thr Leu Glu LeuPro Glu Asp Ala Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr TyrArg Tyr Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Thr Gly Phe Asp Val IleArg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser GluTyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr Lys Val Ala Val Val LeuAsp Arg Arg Ser Leu Arg Leu Gly Ala Pro Glu Leu Leu Lys Glu Gln His Gly TyrLeu Ile Tyr Glu Ile Glu Gly Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn Gln GlySer Thr Val Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile LeuAsp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser IleThr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe Asp Cys Leu Gln IleVal Met Ser Arg Glu Glu Leu Leu Gln Val Asp Leu Ala Ser Gly Glu Ala Arg ValPhe Ser Glu Asp Ala Ser Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His LeuHis Lys Leu Ala Glu Glu His Arg Leu His Gly Gln Phe Val Val Val Asn Asn TyrTyr Arg Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr ThrAsp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly GluAsp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val Asn Ala Gly ThrHis Gln Val Ile Thr Lys Tyr Tyr Pro Thr Leu Leu Glu Asp Pro Asp Ser Asn MetAla Arg Val Glu Gln His Gly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn HisPro Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Ile Val LeuThr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr IleSer Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr Met Asp ArgLeu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val Thr Leu Leu Ala LysAsp Glu Gln Gly Ala Asn Ile Lys Glu Gln Thr Val Tyr Cys Gln Gln Gly Thr GlyGln Thr Asn Asp Thr Ser Ile Glu Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu LeuThr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly Arg Ile Leu Arg Leu LeuGly Asp Gly Ser Thr Cys Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His ProLys Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr Thr Val Ala ValPhe Gly Val Arg Asp Leu Gln Asn Ala Val Val Pro Ile Trp Tyr His Asn Gly LysIle Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe AsnSer Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln ProLeu Val Asp Ser Phe Leu Tyr Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala ProIle Phe Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg Asn Pro ValIle Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val Val Gly Phe AspLys Lys Ser Pro Thr Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg Gln AsnArg Leu Glu Glu Ala Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp AlaIle Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser ArgGlu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly MetAla Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg Arg Arg Val Tyr ValVal Glu Ser Lys Asn Met Ala Lys Asp Lys Gly Glu Phe Ala Ala Ala His Cys PheAla Lys Ser Leu Val Leu Gln Ser Ile Val Pro Glu Glu Ile Thr Ile Pro Thr LeuAla Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile Asp ArgAsp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp GlyGly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu Val Arg Lys PheGln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn Val Val Ile Leu Gln AsnVal Leu Lys Gly Asp Gly Ala Cys Cys Gln Ile Glu Ile Lys Val Gly Asp Ala GluThr Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val AlaSer Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser Asp TyrAla Asp Leu Trp Ala Ile Leu Arg Met Thr*

SEQ ID NO.12：

Met Glu Glu Ala Asp Met Glu Asn Arg Gly Tyr Glu Glu Arg His Ser GlnSer Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Phe Phe Gly Arg SerSer Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu Thr Lys Asn ThrLeu Ser Cys Ser Leu Leu Thr Arg Thr Ala Gln Thr Val Thr Lys Ser Pro Val LeuPro Ala Gln Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu SerGly Cys Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His AspLys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met Tyr Val Asp Phe AsnHis Tyr Arg Tyr Pro Arg Ala His Gly His Gly Arg Asp Thr Gly Gln Ala Val ValCys Met Gln Phe Ala Asp Gly Val Trp Lys Leu Phe Leu Gln Arg His Glu Thr AlaAsn Ser Leu Pro Lys Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser AspAla Glu Pro Arg Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg ArgAla Leu Gln Asp Glu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp Pro Glu IleVal Ser Asn Ala Val Asp Gly Glu Tyr Glu Gly Gly Gln Phe Ser Arg Asn Asp AspAla Ile Arg Gly Trp Asn Glu Leu Arg Ala Ser Met Arg Gln Lys Met Ala Glu ArgLys Lys Arg Leu Glu Arg Asn Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp HisPro Leu Tyr Lys Phe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala TyrAsp Asn Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu IleLys Lys Ala Lys Gly Thr Asn Ala Val Val Asn Ser Gly Ala Ala Pro Lys Gln ProSer Leu Thr Val Gly Glu Arg Tyr Ala Glu Met Glu Ala Ala Leu Trp Met Arg SerAsp Thr Ala Ala Phe Glu Gln Gly Tyr Glu Ser Tyr Gln Thr Leu Arg Val Pro GlyTyr Asn Glu Asp Met Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser LeuThr Asp Glu Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln GluAsn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe Lys Gln Phe SerAsp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp Phe Phe Leu Arg Arg Val Gly AspLys Ser Gly Gly Arg Cys Tyr Pro Leu Val Arg Val Met Ala Val Ala Leu Ser GlnGly Glu Asp Val Ala Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn ProGlu Tyr Ser Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg AspAla Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu ProAla Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser Gln Ala Ser Glu Ser LysVal Tyr Ala Leu Asn Thr Glu Thr His Ser Met Leu Val Ala Val Ile Val Asp LysGln Ser Leu Ala Arg Lys Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Ile Ala Gly PheSer Asp Ile Thr Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln PheAla Gly Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val LeuIle Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly Gly Ile Thr Val Lys GlnLeu Ser Glu Gly Val Asp Leu Thr Asn Val Phe Asp Ser Thr Lys Lys Ala Arg ValVal Thr Glu Thr Ala Ala Arg Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu ThrIle Ile Glu Ala Asp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu LysLeu Lys Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu AsnAla Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr Gly Glu Ile ArgGly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe Lys Ser Tyr Leu Asp Lys AsnLeu Asp Val Leu His Lys Asp Phe Ile Phe Gln Asp Gly Val Phe Lys Pro Leu GluGly Glu Pro Ile Asp Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp TrpPhe Asn Arg Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala GlnLeu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala His ThrAla Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys Ser Ala Leu Tyr Gly GluGln Val Ala Gly Glu Ala Ser Leu Leu Thr Phe Gly Lys Ala Leu Lys Phe Ala AlaThr Glu Gly Leu Gly Val Leu Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr GlnLeu Ser Leu Val Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala PheAsp Ser Ala Ser Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly ValGly Met Ile Leu Gly Gly Ala Gly Val Val Leu Gly Gly Leu Gly Val Gly Phe GlyAla Leu Ala Gln Ala Phe Gly Glu Ile Thr Ala Asp Ala Gln Leu Val Gly Lys TyrPhe Ala Leu Leu Asp Glu Ala Tyr Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys AspLys Ala Leu Met Pro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp LysHis Leu Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Leu ThrGly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro Gln Met Val HisAsp Arg Ser Gln Ala Ile Asn Ile Arg Glu Gln Ile Gly Tyr Lys Asn Asp His AlaThr Leu Glu Leu Pro Glu Asp Ala Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys SerPhe Ile Thr Tyr Gln Tyr Gln Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg GlyPhe Asp Val Ile Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr IlePhe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr Lys ValAla Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala Pro Glu Leu Leu Lys GluGln His Gly Tyr Leu Ile Tyr Glu Ile Glu Gly Thr Gly Gly Gln Tyr Val Ile CysLeu Asn Gln Gly Ser Thr Val Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys ThrLeu Trp Ile Leu Asp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val SerGln Asp Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe AspCys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln Val Asp Leu Ala Ser GlyGlu Ala Arg Val Phe Ser Glu Asp Ala Ser Lys Trp Leu Asp Thr Lys Glu Thr IleHis Glu His Leu His Lys Leu Ala Glu Glu His Arg Leu His Gly Gln Phe Val ValVal Asn Asn Tyr Tyr Arg Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp ArgMet Leu Tyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala GlyVal Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg ValAsn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Leu Leu Glu Asp ProAsp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn Ile Val Val Met Ala Met SerArg Leu Asn His Pro Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln GluSer Met Val Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg GlnThr Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val SerThr Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val ThrIle Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp Leu Arg Thr Val AspGly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr Asp Leu Thr Leu ThrVal Ser Ser His Lys Val Ala Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys GluGln Thr Val Tyr Cys Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Ile Glu SerIle Pro Ile Pro Gly Leu Ser Asn Leu Leu Thr Val Tyr Gly Glu Val Phe Ala ThrThr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr Tyr Leu Asp GlyIle Asn Glu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala Thr LeuGlu Ala Glu Lys Ala Thr Thr Val Ala Val Phe Gly Val Arg Asp Leu Gln Asn AlaVal Val Pro Ile Trp Tyr His Asn Gly Lys Ile Ala Val Ala Ser Ser Lys Leu TyrGly Lys Gln Leu Gln Phe Leu Gly Phe Asn Ser Asn Gly Glu Ala Trp Leu Phe AspLeu Asp Asp Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Ser Phe Leu Tyr Ala IlePhe Ser Leu Asp Gly Lys Leu Ile Ala Pro Ile Phe Ile Pro Gln Val Glu Gln MetLeu Ser Gly Gln His Leu Arg Asn Pro Val Ile Val Asp Gly Arg Leu His Val ThrThr Ile Asp Gly Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala ValAsn Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly Asn LeuLeu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln Gly Asp Pro Glu SerAla Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile Val Thr Ala Asn Gly Leu ThrTrp Ala Asp His Pro Leu Trp Leu Gly Met Ala Val Gly Ala Ala Thr Pro Tyr IleTyr Ser Thr Ser Arg Arg Arg Val Tyr Ala Val Glu Ser Lys His Met Ala Lys AspLys Gly Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser IleVal Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Ile Asp Phe Ala Leu Val AlaAla Thr Asn Ala Thr Ile Val Ile His Arg Asp Asn Trp Lys His Tyr Lys Ala IleVal Leu Asp Asn Lys Val Asp Gln Asp Gly Gly Ala Lys Val Val Val Gly Ser GlyIle Pro Met Lys Val Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp ProAsn Asn Gly Asn Val Val Ile Leu Arg Asn Val Leu Lys Gly Asp Gly Ala Cys SerGln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp Phe Ser GluIle Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val Phe Glu Leu Gly Asp ValIle Ala Val Thr Lys His Lys Ser Asp Tyr Ala Asp Leu Trp Ala Ile Leu Arg MetThr

SEQ ID NO.13：

Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn Phe Met GluAsn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly Glu Ser Ala Ser Ser GlnAla Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala Gly Leu His Ala Asn Val GluAla Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala ThrMet Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His SerMet Leu Ala Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr Tyr Phe TyrAsp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe Ser Glu Ala LeuLys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr Lys Ala Phe Gly Thr AlaGlu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr Thr Ala Met Ala Asp Ala HisVal Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn ValPhe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg Leu Ala GluAsp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln Trp Ala Asn ArgTrp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu Gly Leu Ser Gly Glu Trp MetPro Leu Leu Glu Arg Met Gln Glu Asn Gly His Gly Ser Tyr Gln Ile Glu Phe LeuAsn Thr Lys Asn Thr Gly Glu Thr Arg Trp Val Ser Thr Arg Asp Ala Thr Phe LysGlu Phe Lys Ser Tyr Leu Asp Lys Asn Phe Asp Ala Leu Arg Lys Asp Phe Ile PheGln Asp Gly Val Phe Lys Pro Leu Asp Gly Glu Pro Ile Asp Gly Leu Asn Ala MetPhe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Pro Gly Thr Asn Ala Glu Glu AspAla Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu Ala Ile Lys Val His Ser TyrIle Asn Met Ala Gln Val Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr AsnLeu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Glu Ala Ser Leu Leu ThrPhe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu Leu Gly AlaSer Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn Asp Val Gln LysAla Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala Ser Phe Leu Ala Gly Ile GlyAla Ile Val Ala Gly Ala Ser Gly Val Gly Met Ile Leu Gly Gly Ala Gly Val IleLeu Gly Gly Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Gln Ile ThrAla Asp Ala Gln Leu Val Gly Glu Tyr Phe Ala Leu Leu Asp Glu Ala Tyr Ser GlyGly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu Ala Gly Ala ValIle Asp Lys Ile Arg Leu Ala Asp Lys Arg Leu Gln Val His Phe Asp Ser Gln PheIle Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe TrpVal Gly Asp Met Pro Gln Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg GluGln Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser AspLeu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr Glu Ile Leu ProPhe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg Leu Glu Glu AspTyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser Glu Tyr Thr Ile Arg Thr IleLys His Glu Tyr Val Glu Thr Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu ArgLeu Gly Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile GluGly Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn His Gly Ser Thr Val Thr Leu LysThr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp Ala Arg Gln Leu IleAla Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser Ile Thr Ile Gly Ser Thr ThrVal His Leu Arg Asn Gln Asp Phe Asp Cys Leu Gln Ile Val Met Ser Arg Asp GluLeu Leu Gln Val Asp Leu Ala Ser Arg Glu Val Arg Val Phe Ser Glu Asp Ala SerLys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala Gly GluHis Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr Arg Gly Val Gly ArgAla Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr Thr Asp Val Glu Asp Glu ThrLeu Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly Glu Asp Ala Tyr Phe Tyr AsnVal Asn Gln Asn Ala Leu Trp Arg Val Asn Ala Asp Thr His Gln Val Ile Thr LysTyr Tyr Pro Thr Leu Leu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln HisGly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp Lys Gly Val AsnGly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu Thr Ala Val Ile Gly AsnSer Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr Ile Ser Leu Thr Glu Leu ArgGly Val Asn Asp Met Ala Leu Val Ser Thr Met Asp Arg Leu Pro Gly Gln Lys ValVal Ala Thr Asn Ala Glu Phe Val Thr Leu Leu Ala Lys Asp Glu Gln Gly Ser GlnVal Cys Phe Trp Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln ProPro Ile Pro Thr Asp Leu Thr Leu Thr Val Ser Ser His Lys Val Ala Gly Gln AspVal Phe Tyr Phe Tyr Ser Ile Lys Glu Gln Thr Val Tyr Cys Gln Gln Gly Thr GlyGln Thr Asn Asp Thr Ser Ile Glu Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu LeuThr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly Arg Ile Leu Arg Leu LeuGly Asp Gly Ser Thr Tyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His ProLys Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr Thr Val Ala ValPhe Gly Val Arg Asp Pro Gln Asn Ala Val Val Pro Ile Trp Tyr His Asn Gly LysIle Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe AsnSer Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln ProLeu Val Asp Ser Gln Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile AlaPro Asn Leu Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg Asn ProVal Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val Val Gly PheAsp Lys Lys Ser Pro Thr Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg GlnAsn Arg Leu Glu Glu Ala Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn AspAla Ile Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro SerArg Glu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu GlyMet Ala Val Gly Ala Ala Thr Leu Tyr Ile Tyr Ser Thr Ser Arg Arg Arg Val TyrAla Val Glu Ser Lys Lys Met Ala Lys Asp Lys Gly Glu Phe Ala Ala Ala His CysPhe Ala Lys Ser Leu Val Leu Gln Ser Ile Val Pro Glu Glu Ile Thr Ile Pro ThrLeu Ala Gly Thr Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile AspArg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln AspGly Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu Val Arg LysPhe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn Val Val Ile Leu GlnAsn Val Leu Lys Gly Asp Gly Ala Cys Ser Lys Ile Glu Ile Lys Val Gly Asp AlaGlu Thr Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu ValAla Ser Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser AspTyr Ala Asp Leu Trp Ala Ile Leu Arg Met Thr

SEQ ID NO.14：

Met Glu Glu Val Asp Met Gln Asn Arg Gly Tyr Glu Glu Trp His Ser GlnSer Gln Ser Ala Thr Thr Leu Arg Arg Arg Lys Arg Ser Pro Val Phe Gly Arg SerSer Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu Thr Gln Asn ThrLeu Ser Cys Ser Leu Leu Thr Arg Thr Ala Glu Thr Val Thr Lys Ser Pro Val LeuPro Glu Arg Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu PheVal Gln Val Val Ala Glu Gln Ser Tyr Asp Thr Val Lys Ala Tyr Leu Leu Gly TyrAsn Gly Glu Asn Asp Ser Pro Asn Pro Ala Tyr Val Asp Ile Pro Lys Arg Ala AlaGlu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys Ser Val Val Val ThrGln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp Lys Arg Val Asn Ser Ser IleLeu Tyr Asp Asn Val Val Met Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg AlaHis Gly His Gly Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp GlyVal Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile TrpSer Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro Arg Gln His MetGlu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala Leu Gln Asp Glu Leu LysLys Phe Ala Asp Met Phe Gly Val Asp Pro Glu Ile Val Ser Asn Ala Val Asp GlyLys Tyr Lys Gly Gly Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn GluLeu Arg Ala Ser Met Arg Gln Lys Met Ala Glu Arg Asn Lys Val Leu Leu Thr MetLys Leu Glu Gln Pro Arg Ser Ser Arg Val Leu Pro Leu Val Asn Ala Thr Gln ArgTrp Arg Arg Pro Phe Gly Cys Val Ala Thr Gln Arg Arg Leu Ser Lys Ala Gly ArgLeu Val Gln Ala Tyr Ile His Arg Asp Gln Glu Asn His Leu Leu Thr Gln Lys ThrGln Glu Ile Glu Ser Thr Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser AlaPro Gln Asp Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr ProLeu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn PheMet Glu Asn Leu Arg Thr Leu Ser Lys Asn Pro Glu Tyr Ser Gly Glu Ser Ala SerSer Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala Gly Leu His Ala AsnVal Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp ValAla Thr Met Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu ThrHis Ser Met Leu Ala Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr TyrPhe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe Ser GluAla Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr Lys Pro Phe GlyThr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr Thr Val Met Ala AspAla His Val Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu ThrAsn Val Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg LeuAla Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln Trp AlaAsn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu Gly Leu Ser Gly GluTrp Met Pro Leu Leu Glu Lys Met Gln Glu Asn Ala Asp Gly Ser Tyr Gln Ile AspPhe Ile Asn Thr Lys Asn Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Gly ThrPhe Lys Glu Leu Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys Asp PheIle Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly Leu AsnAla Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg Gly Thr Asn Ala GluGlu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu Ala Ile Lys Val HisSer Tyr Ile Asn Met Ala Gln Val Ala His Thr Ala Val Thr Asp Val Ala Lys ValTyr Ser Leu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala Ser LeuLeu Thr Phe Gly Lys Ala Leu Lys Leu Ala Ala Thr Glu Gly Leu Gly Val Leu LeuGly Ala Pro Val Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn Asp ValGln Lys Ala Ile Ile Gly Thr Gln Leu Asp Phe Asp Ser Ala Ser Phe Leu Val SerIle Gly Ala Ala Val Ala Gly Val Ser Gly Val Gly Ile Val Leu Ser Gly Ala GlyVal Leu Leu Ala Gly Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly GluIle Thr Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala TyrArg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu Ala GlyAla Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln Val His Phe Asp SerGln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly Lys Ile Asn Tyr PhePhe Trp Val Gly Asp Met Pro Lys Met Val His Asp Arg Ser Gln Ala Ile Asn ValArg Glu Gln Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp AlaSer Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr Glu IleLeu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg Leu GluGlu Asp His Arg Phe Asp Tyr Val Phe Tyr Ile Phe Pro Ser Glu Tyr Thr Ile ArgThr Ile Lys His Glu Tyr Val Glu Thr Lys Val Ala Val Val Pro Asp Arg Arg ProLeu Arg Leu Arg Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr GluIle Glu Gly Thr Gly Gly Glu Tyr Val Ile Cys Leu Asn Lys Gly Ser Thr Val ThrLeu Phe Thr Thr Gly Ser Glu Ala Asn Lys Thr Ser Trp Ile Leu Asp Ala Arg GlnIle Ile Ala Gly Asn Asp Pro Ile Ile Val Ser Gln Asp Ser Ile Thr Met Gly SerThr Thr Val His Leu Arg Asn His Asp Phe Asp Cys Leu Arg Ile Val Thr Ser ArgGlu Glu Leu Leu Gln Val Asp Ser Ala Ser Arg Gln Thr Arg Val Leu Ser Glu AspAla Ser Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu AlaGlu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr Ser Val Gln GluGln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly Arg Ala His Asp Arg Met LeuTyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val ThrGly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val Asn AlaGly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Val Leu Glu Asp Pro Asp SerLys Met Ala Arg Val Glu Gln Arg Gly Asn Ile Val Val Met Ala Met Ser Arg LeuAsn His Ser Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser MetVal Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Ala SerThr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr MetAsp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val Thr Ile LeuAla Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp Leu Arg Thr Val Asp Gly ValLeu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr Asp Leu Thr Leu Thr Val SerSer His Lys Val Ala Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys Glu Gln ArgVal Tyr Arg Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Val Gly Ser Ile ProIle Pro Gly Leu Ser Asn Leu Phe Thr Val Tyr Gly Glu Val Phe Ala Thr Thr AsnAsp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr Tyr Leu Glu Gly Ile AsnGlu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala Thr Leu Glu AlaGlu Lys Ala Thr Thr Ile Ala Val Phe Gly Val Arg Asp Pro Gln Asn Ala Val ValPro Ile Cys Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Gly Gly Ile Leu Tyr SerGln Pro Leu Val Asp Ser Gln Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys LeuIle Ala Pro Asn Leu Ile Pro Gln Val Gln Gln Met Leu Ser Gly Gln His Leu ArgAsn Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val ValGly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Gly Asn Lys Ala Trp Gln Ile SerArg Gln Asn Arg Leu Glu Glu Ala Leu Arg Asn Leu Leu Ser Asn Trp Ser Asp HisAsn Asp Ala Ile Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr ValPro Ser Arg Glu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu TrpLeu Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg Cys ArgVal Tyr Ala Val Glu Ser Lys Asn Met Ala Lys Asp Lys Gly Glu Phe Ala Ala AlaHis Cys Phe Ala Lys Ser Leu Val Leu Gln Ser Ile Val Pro Ala Glu Ile Thr IlePro Met Leu Ala Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Lys Ala Thr Ile ValIle Asp Arg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val AspGln Asp Gly Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu ValArg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn Val Val IleLeu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Ser Gln Ile Glu Ile Lys Val GlyAsp Ala Glu Thr Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His ProLeu Val Ala Ser Gln Val Phe Glu Leu Gly Asp Gly Cys Asp Cys Ser His Lys ThrGln Ile Gly Leu Cys Gly Ser Val Gly His Ser Thr His Asp Ile Ala Tyr Ile SerHis Ala Tyr Leu His Arg Trp Pro Ile Tyr Val Ile Ser Thr Leu Ile Ala

SEQ ID NO.15：

Met Glu Glu Ala Asp Met Glu Asn Arg Gly Asn Glu Glu Arg His Ser GlnSer Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Val Phe Gly Arg SerSer Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu Thr Arg Asn ThrLeu Ser Cys Ser Leu Leu Thr Arg Thr Ala Glu Ala Val Thr Lys Ser Pro Ala LeuPro Ala Gln Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly Asp Val Leu PheVal Gln Leu Val Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr Leu Leu Gly TyrAsn Gly Glu Asn Asp Phe Pro Lys Pro Ala Tyr Val Asp Ile Pro Lys Arg Gly AlaLys Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys Ser Val Val Val ThrGln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp Lys Arg Val Asn Ser Ser IleLeu Tyr Asp Asn Val Val Met Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg AlaHis Gly His Gly Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp GlyVal Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile TrpSer Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Asp Pro Arg Pro His MetGlu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala Leu Gln Asp Glu Leu LysLys Phe Ala Asp Met Phe Gly Val Asp Pro Glu Ile Val Ser Asn Ala Val Asp GlyLys Tyr Glu Gly Gly Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn GluLeu Arg Ala Arg Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu Leu Glu Thr LysLys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr Glu Phe Arg LysGln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp Asn Thr Leu Glu Ala SerGlu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu Ile Lys Lys Ala Arg Gly Thr AsnAla Val Val Asn Ser Gly Ser Ala Pro Lys Gln Pro Ser Leu Thr Val Gly Glu ArgTyr Ala Glu Met Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu GlnGly Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu Asp Met Thr SerIle Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp Glu Gln Leu Gly AlaLeu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln Glu Asn His Leu Leu Arg Gln LysThr Gln Glu Ile Glu Asn Thr Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu SerAla Pro Gln Asp Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys TyrPro Leu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln AsnPhe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly Glu Ser AlaSer Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala Gly Leu His AlaAsn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe Gly Leu Pro AspVal Ala Thr Met Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr GluThr His Ser Met Leu Val Ala Val Ile Ala Asp Glu Gln Ser Leu Ala Pro Lys TyrTyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe SerGlu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr Lys Ala PheGly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr Thr Ala Met AlaAsp Ala His Val Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp LeuThr Asn Val Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala ArgLeu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln TrpAla Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Arg Leu Lys Glu Gly Leu Ser GlyGlu Trp Met Pro Leu Leu Glu Lys Met Gln Glu Asn Ala Asp Gly Ser Tyr Gln IleAsp Phe Ile Asn Thr Lys Asn Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp AlaThr Phe Lys Glu Phe Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys AspPhe Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly LeuAsn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg Gly Thr Asn AlaGlu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu Ala Ile Lys ValHis Ser Tyr Ile Asn Met Ala Gln Val Ala His Thr Ala Val Thr Asp Val Ala LysVal Tyr Ser Leu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala SerLeu Leu Thr Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val LeuLeu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn AspVal Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala Ser Phe Leu ValSer Ile Gly Ala Ala Val Ala Gly Val Ser Gly Val Gly Ile Val Leu Ser Gly AlaGly Val Leu Leu Ala Gly Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe GlyGln Ile Thr Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu AlaTyr Ser Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu AlaGly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln Val His Phe AspSer Gln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly Lys Ile Asn TyrPhe Phe Trp Ala Gly Asp Met Pro Gln Met Val His Asp Arg Ser Gln Ala Ile AsnVal Arg Glu Gln Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu AspAla Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr GluIle Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg LeuGlu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser Glu Tyr Thr IleArg Thr Ile Lys His Glu Tyr Val Glu Thr Lys Val Ala Val Val Leu Asp Arg ArgSer Leu Arg Leu Gly Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile TyrGlu Ile Glu Gly Thr Gly Gly Gln Tyr Val Val Cys Leu Asn His Gly Ser Thr ValThr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp Ala ArgGln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser Ile Thr Ile GlySer Thr Thr Val His Leu Arg Asn Gln Asp Phe Asp Cys Leu Gln Ile Val Met SerArg Glu Glu Leu Leu Gln Val Asp Leu Ala Ser Arg Gln Ala Arg Val Leu Ser GluAsp Ala Ser Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys LeuAla Glu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr Ser Val GlnGlu Gln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly Arg Ala Tyr Tyr Asp AlaAla His Asp Arg Met Leu Tyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp AlaVal Leu Ala Gly Val Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn AlaLeu Trp Arg Val Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr ValLeu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn Ile Val ValMet Ala Met Ser Arg Leu Asn Gln Pro Asp Lys Gly Val Asp Gly Ile Ile Tyr LeuVal His Gln Glu Ser Met Val Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu LysArg Leu Arg Gln Thr Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp MetAla Leu Val Ser Thr Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn AlaGlu Phe Val Thr Ile Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp LeuArg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr AspLeu Ala Leu Thr Val Ser Ser His Lys Val Ala Gly Gln Asp Val Phe Tyr Phe CysSer Ile Lys Glu Gln Thr Val Tyr Arg Gln Gln Gly Thr Gly Gln Thr Asn Asp ThrSer Val Gly Ser Ile Pro Ile Gln Gly Leu Ser Asn Leu Phe Thr Val Tyr Gly GluVal Phe Ala Thr Thr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser ThrTyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln AspLeu Ala Thr Leu Glu Ala Glu Thr Ala Thr Thr Ile Ala Val Phe Gly Val Arg AspPro Gln Asn Ala Val Val Pro Ile Trp Tyr His Ser Gly Lys Ile Ala Val Ala SerSer Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe His Ser Asn Asp Gln AlaTrp Leu Phe Asp Leu Asp Gly Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Arg GlnThr Val Asp Thr Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro Asp Leu Ile ProGln Val Gln Gln Met Leu Ser Gly Gln His Leu Arg Asn Pro Val Ile Val Asp GlyArg Leu His Val Thr Thr Ile Asp Gly Val Val Val Gly Phe Asp Lys Lys Ser ProThr Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu GluAla Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu GlnGly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile Val ThrAla Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly Met Ala Val Gly AlaAla Thr Pro Tyr Ile Tyr Asn Thr Ser Pro Arg Arg Arg Ser Ile Val Pro Glu GluIle Thr Ile Pro Thr Leu Ala Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Asn AlaThr Ile Val Ile Asp Arg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp AsnLys Val Asp Gln Asp Gly Gly Ala Lys Val Val Leu Gly Ser Glu Ile Pro Met LysVal Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly AsnVal Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Cys Gln Ile Asp IleLys Val Gly Asp Ala Glu Thr Met Leu Ala Ser Asp Phe Ser Glu Ile Phe Ser LysThr His Pro Leu Val Ala Ser Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val ThrLys His Lys Ser Asp Tyr Glu Asp Leu Trp Ala Ile Leu His Met Thr

实施例2：35s启动子+苏铁毒蛋白基因的植物表达载体的构建

线性化载体制备：使用限制性内切酶BstBI和MluI双酶切VM062-3*FLAG载体，使环状载体线性化。

带有同源臂的目标序列扩增：

SEQ ID NO.17：

以正向引物：

5’-gggcggtaccccgggttcgaaATGGTTATGGTAATTGACTATCCAAGTC-3’；

反向引物：

5’-cactagttaattaagacgcgtCTATGTCATGCGTAGAATGGCCC-3’为引物，对苏铁毒蛋白基因进行PCR扩增，扩增体系为：

KOD One

上游引物(10μM each)1.5μL

下游引物(10μM each)1.5μL

模板2μL

灭菌水20μL

总体积50μL

PCR的程序为98℃5min；98℃30s，50℃30s，68℃3min，30个循环；然后68℃10min，4℃保存。

载体酶切产物与PCR产物的检测与回收：琼脂糖凝胶电泳检测PCR产物与载体酶切产物，使用

胶回收产物重组：使用

2×Basic Assembly Mix 5μL

酶切载体XμL

PCR产物YμL

灭菌水to 10μL

*10μL反应体系中，载体与各个插入片段加入量建议均在0.01～0.25pmols，载体与各个插入片段的最佳摩尔比为1：2。

轻轻混合，50℃反应15分钟。

转化方法：

1.在冰上融化Trans1-T1感受态细胞，静置2-5分钟，观察细胞是否已解冻，用手指轻弹管底1-2次。

2.在50μL细胞中加入2μL的重组产物，轻轻弹动离心管壁混匀后，在冰上放置30分钟。

3.42℃水浴中热激30秒，之后马上转至冰上冷却2分钟。

4.加入450μL常温无抗LB液体培养基，之后37℃摇床中250rpm培养1小时。

5. 5000rpm离心2分钟，收集菌体，留100μL液体重悬，将其均匀涂布于含有卡那霉素抗性的LB固体平板上，之后37℃培养箱过夜培养。

测序鉴定：经菌落PCR和质粒PCR筛选阳性克隆，送至上海生工生物工程有限公司测序分析，结果表明：成功得到了苏铁毒蛋白基因植物超表达载体VM062-Suit(见图2)。

本发明选用VM062-3*FLAG载体作为基础植物表达载体，由于35S启动子与3*FLAG之间酶切位点易发生移码突变，加上标签位于蛋白N端有利于蛋白表达，因此本发明采用3*FLAG标签之后的BstBI和MluI酶切位点，用于构建最终表达载体。载体序列如序列SEQ IDNO.18。

SEQ ID NO.18植物表达载体序列：

GAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGCTAGAGCAGCTTGCCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTGAAATCACCAGTCTCTCTCTACAAATCTATCTCTCTCGAGTCTACCATGGCTCAGATCAGGTCGATGGCTCAGGGCATTCAGACGCTGTCGCTCAACTCCTCTAATCTCTCCAAGACGCAGAAGGGGCCGCTCGTGTCGAACTCTCTCTTCTTCGGCAGCAAGAAGCTGACACAGATCTCTGCCAAGTCACTGGGGGTTTTCAAGAAGGACTCGGTGCTCCGGGTGGTCCGCAAGTCCAGCTTCCGCATCTCAGCTTCCGTCGCTACAGCTGAGGCTGAGGCCGTGATCGCTGAGGTCTCCACTCAGCTCAGCGAGGTTGTGGGCGTGATCGAGAGGCACCTGGAGCCAACCCTCCTGGCTGTCCATCTCTACGGGTCAGCGGTTGATGGTGGCCTGAAGCCCCACTCCGACATCGATCTCCTGGTTACAGTGACTGTCCGGCTGGACGAGACCACGAGGAGGGCTCTCATTAACGATCTCCTGGAGACCAGCGCTTCGCCAGGCGAGTCCGAGATCCTCAGGGCGGTTGAGGTGACGATTGTCGTTCATGACGATATCATTCCATGGAGGTACCCAGCTAAGAGGGAGCTCCAGTTCGGCGAGTGGCAGCGCAATGACATCCTGGCCGGGATTTTCGAGCCAGCGACAATCGACATTGATCTGGCTATCCTCCTGACTAAGGCTAGGGAGCACTCCGTCGCTCTGGTTGGCCCTGCTGCTGAGGAGCTCTTCGACCCAGTCCCTGAGCAGGATCTCTTCGAGGCCCTGAACGAGACCCTCACGCTGTGGAATTCTCCGCCCGACTGGGCTGGCGATGAGAGGAATGTGGTCCTCACCCTGTCGCGCATCTGGTACTCTGCTGTCACGGGGAAGATTGCTCCAAAGGACGTGGCTGCTGATTGGGCGATGGAGAGGCTGCCAGCTCAGTACCAGCCTGTGATCCTCGAGGCTAGGCAGGCTTACCTGGGCCAGGAGGAGGACAGGCTCGCGTCCCGGGCTGATCAGCTGGAGGAGTTCGTTCATTACGTCAAGGGCGAGATTACTAAGGTCGTTGGCAAGTGACTCGAGTTTCTCCATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTACTAAAATCCAGATCCCCCGAATTAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCAGCTCGGCACAAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGTTGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAGCGTTGCTGCCTGTGATCACCGCGGTTTCAAAATCGGCTCCGTCGATACTATGTTATACGCCAACTTTGAAAACAACTTTGAAAAAGCTGTTTTCTGGTATTTAAGGTTTTAGAATGCAAGGAACAGTGAATTGGAGTTCGTCTTGTTATAATTAGCTTCTTGGGGTATCTTTAAATACTGTAGAAAAGAGGAAGGAAATAATAAATGGCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCGCTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGGTGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAAGGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGAAGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCTGGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAGTATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTGCATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATAGCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTGGCCGATGTGGATTGCGAAAACTGGGAAGAAGACACTCCATTTAAAGATCCGCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTGTCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGCAAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTGGTATGACATTGCCTTCTGCGTCCGGTCGATCAGGGAGGATATCGGGGAAGAACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGGGAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGTACCTAGAATGCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGGGTGCCTTGATGTGGGCGCCGGCGGTCGAGTGGCGACGGCGCGGCTTGTCCGCGCCCTGGTAGATTGCCTGGCCGTAGGCCAGCCATTTTTGAGCGGCCAGCGGCCGCGATAGGCCGACGCGAAGCGGCGGGGCGTAGGGAGCGCAGCGACCGAAGGGTAGGCGCTTTTTGCAGCTCTTCGGCTGTGCGCTGGCCAGACAGTTATGCACAGGCCAGGCGGGTTTTAAGAGTTTTAATAAGTTTTAAAGAGTTTTAGGCGGAAAAATCGCCTTTTTTCTCTTTTATATCAGTCACTTACATGTGTGACCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGGGTTCCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGTGCTATCCACAGGAAAGAGACCTTTTCGACCTTTTTCCCCTGCTAGGGCAATTTGCCCTAGCATCTGCTCCGTACATTAGGAACCGGCGGATGCTTCGCCCTCGATCAGGTTGCGGTAGCGCATGACTAGGATCGGGCCAGCCTGCCCCGCCTCCTCCTTCAAATCGTACTCCGGCAGGTCATTTGACCCGATCAGCTTGCGCACGGTGAAACAGAACTTCTTGAACTCTCCGGCGCTGCCACTGCGTTCGTAGATCGTCTTGAACAACCATCTGGCTTCTGCCTTGCCTGCGGCGCGGCGTGCCAGGCGGTAGAGAAAACGGCCGATGCCGGGATCGATCAAAAAGTAATCGGGGTGAACCGTCAGCACGTCCGGGTTCTTGCCTTCTGTGATCTCGCGGTACATCCAATCAGCTAGCTCGATCTCGATGTACTCCGGCCGCCCGGTTTCGCTCTTTACGATCTTGTAGCGGCTAATCAAGGCTTCACCCTCGGATACCGTCACCAGGCGGCCGTTCTTGGCCTTCTTCGTACGCTGCATGGCAACGTGCGTGGTGTTTAACCGAATGCAGGTTTCTACCAGGTCGTCTTTCTGCTTTCCGCCATCGGCTCGCCGGCAGAACTTGAGTACGTCCGCAACGTGTGGACGGAACACGCGGCCGGGCTTGTCTCCCTTCCCTTCCCGGTATCGGTTCATGGATTCGGTTAGATGGGAAACCGCCATCAGTACCAGGTCGTAATCCCACACACTGGCCATGCCGGCCGGCCCTGCGGAAACCTCTACGTGCCCGTCTGGAAGCTCGTAGCGGATCACCTCGCCAGCTCGTCGGTCACGCTTCGACAGACGGAAAACGGCCACGTCCATGATGCTGCGACTATCGCGGGTGCCCACGTCATAGAGCATCGGAACGAAAAAATCTGGTTGCTCGTCGCCCTTGGGCGGCTTCCTAATCGACGGCGCACCGGCTGCCGGCGGTTGCCGGGATTCTTTGCGGATTCGATCAGCGGCCGCTTGCCACGATTCACCGGGGCGTGCTTCTGCCTCGATGCGTTGCCGCTGGGCGGCCTGCGCGGCCTTCAACTTCTCCACCAGGTCATCACCCAGCGCCGCGCCGATTTGTACCGGGCCGGATGGTTTGCGACCGTCACGCCGATTCCTCGGGCTTGGGGGTTCCAGTGCCATTGCAGGGCCGGCAGACAACCCAGCCGCTTACGCCTGGCCAACCGCCCGTTCCTCCACACATGGGGCATTCCACGGCGTCGGTGCCTGGTTGTTCTTGATTTTCCATGCCGCCTCCTTTAGCCGCTAAAATTCATCTACTCATTTATTCATTTGCTCATTTACTCTGGTAGCTGCGCGATGTATTCAGATAGCAGCTCGGTAATGGTCTTGCCTTGGCGTACCGCGTACATCTTCAGCTTGGTGTGATCCTCCGCCGGCAACTGAAAGTTGACCCGCTTCATGGCTGGCGTGTCTGCCAGGCTGGCCAACGTTGCAGCCTTGCTGCTGCGTGCGCTCGGACGGCCGGCACTTAGCGTGTTTGTGCTTTTGCTCATTTTCTCTTTACCTCATTAACTCAAATGAGTTTTGATTTAATTTCAGCGGCCAGCGCCTGGACCTCGCGGGCAGCGTCGCCCTCGGGTTCTGATTCAAGAACGGTTGTGCCGGCGGCGGCAGTGCCTGGGTAGCTCACGCGCTGCGTGATACGGGACTCAAGAATGGGCAGCTCGTACCCGGCCAGCGCCTCGGCAACCTCACCGCCGATGCGCGTGCCTTTGATCGCCCGCGACACGACAAAGGCCGCTTGTAGCCTTCCATCCGTGACCTCAATGCGCTGCTTAACCAGCTCCACCAGGTCGGCGGTGGCCCATATGTCGTAAGGGCTTGGCTGCACCGGAATCAGCACGAAGTCGGCTGCCTTGATCGCGGACACAGCCAAGTCCGCCGCCTGGGGCGCTCCGTCGATCACTACGAAGTCGCGCCGGCCGATGGCCTTCACGTCGCGGTCAATCGTCGGGCGGTCGATGCCGACAACGGTTAGCGGTTGATCTTCCCGCACGGCCGCCCAATCGCGGGCACTGCCCTGGGGATCGGAATCGACTAACAGAACATCGGCCCCGGCGAGTTGCAGGGCGCGGGCTAGATGGGTTGCGATGGTCGTCTTGCCTGACCCGCCTTTCTGGTTAAGTACAGCGATAACCTTCATGCGTTCCCCTTGCGTATTTGTTTATTTACTCATCGCATCATATACGCAGCGACCGCATGACGCAAGCTGTTTTACTCAAATACACATCACCTTTTTAGACGGCGGCGCTCGGTTTCTTCAGCGGCCAAGCTGGCCGGCCAGGCCGCCAGCTTGGCATCAGACAAACCGGCCAGGATTTCATGCAGCCGCACGGTTGAGACGTGCGCGGGCGGCTCGAACACGTACCCGGCCGCGATCATCTCCGCCTCGATCTCTTCGGTAATGAAAAACGGTTCGTCCTGGCCGTCCTGGTGCGGTTTCATGCTTGTTCCTCTTGGCGTTCATTCTCGGCGGCCGCCAGGGCGTCGGCCTCGGTCAATGCGTCCTCACGGAAGGCACCGCGCCGCCTGGCCTCGGTGGGCGTCACTTCCTCGCTGCGCTCAAGTGCGCGGTACAGGGTCGAGCGATGCACGCCAAGCAGTGCAGCCGCCTCTTTCACGGTGCGGCCTTCCTGGTCGATCAGCTCGCGGGCGTGCGCGATCTGTGCCGGGGTGAGGGTAGGGCGGGGGCCAAACTTCACGCCTCGGGCCTTGGCGGCCTCGCGCCCGCTCCGGGTGCGGTCGATGATTAGGGAACGCTCGAACTCGGCAATGCCGGCGAACACGGTCAACACCATGCGGCCGGCCGGCGTGGTGGTGTCGGCCCACGGCTCTGCCAGGCTACGCAGGCCCGCGCCGGCCTCCTGGATGCGCTCGGCAATGTCCAGTAGGTCGCGGGTGCTGCGGGCCAGGCGGTCTAGCCTGGTCACTGTCACAACGTCGCCAGGGCGTAGGTGGTCAAGCATCCTGGCCAGCTCCGGGCGGTCGCGCCTGGTGCCGGTGATCTTCTCGGAAAACAGCTTGGTGCAGCCGGCCGCGTGCAGTTCGGCCCGTTGGTTGGTCAAGTCCTGGTCGTCGGTGCTGACGCGGGCATAGCCCAGCAGGCCAGCGGCGGCGCTCTTGTTCATGGCGTAATGTCTCCGGTTCTAGTCGCAAGTATTCTACTTTATGCGACTAAAACACGCGACAAGAAAACGCCAGGAAAAGGGCAGGGCGGCAGCCTGTCGCGTAACTTAGGACTTGTGCGACATGTCGTTTTCAGAAGACGGCTGCACTGAACGTCAGAAGCCGACTGCACTATAGCAGCGGAGGGGTTGGATCAAAGTACTTTGATCCCGAGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCGTTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACCCGCCAATATATCCTGTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCCAAGCTCAAGCTGCTCTAGCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTAAGCTTGCATGCCTGCAGGTCCCCAGATTAGCCTTTTCAATTTCAGAAAGAATGCTAACCCACAGATGGTTAGAGAGGCTTACGCAGCAGGTCTCATCAAGACGATCTACCCGAGCAATAATCTCCAGGAAATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCACAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAAAAGGTAGTTCCCACTGAATCAAAGGCCATGGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGAACACGGGGGACTCTAGAGGATCGCGAGGCGCGCCGTCGACATGGACTACAAGGACGACGATGATAAGGGCATGGACTACAAGGACGACGATGATAAGGGCATGGACTACAAGGACGACGATGATAAGGGCGGTACCCCGGGttcgaaATGGTTATGGTAATTGACTATCCAAGTCTCAGAGCTTCGTTATATGCCACTAAGAGTGCGGTCCCATGTCGCCCGCTTCCGGGCAATCCAGAGCGGGTGTCGCGCCGGGTATCACGCAGAGGCGCGGGAGAGATCGGGTCCCATGCAGATTTCTGGATCTCCCGCGCCAAGGCATCCGCCATCCGGATTTTCCCGCGTCAAGAGGGTCCGCTCCGTGAATCCCGGAGAAGATTCTATCTTCTCCGCGAAGCGGTGTTCACCGGCCGCAGGACCGCCTGGGCCGCTAATCCAGCGGCCCAACGGGCAGCATGGGACCGCAAGAGATCCCCTTTCTTCGGAAGGTCTTCCGATGATATTCATTCATTCAGCAAGAATCCAGAAGAATTCTTGACTCAAAACACTCTCAGCTGCAGTTTATTAACTCGCACCGCTCAGACCGTCACAAAAAGCCCAGTATTGCCCGCCCAAGGTTGGGTCAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGCTCTCTTTGTTGAGCTGGTTGCCGAGAAATCAGATGAGACAGTGAAGGCGTACTTACTTGGCTACAATGGTAAGAACGACTCCCCCGAGCCGGCATACGTTGATATTCCAAAGAGGGGCGCCGAGAACGGAACATTTTTGTTTACCGTGGATCTCTCGGGCTGCTCCGTTGTTGTGACACAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCACGGACACGGGAGAGATACGGGACAAGCGGTCGTCTGTATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGAACCCCGTCCACATATGGAAGATGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAATTGAAGCACTTCGCCAACACGCTTGAAGTGGACCCGGACATAGTGTCCAACGCTGTCGACGGCGAATACGAAGGCGGCCAGTTTTCCAGGAACGACGATGCAATTCGTGGATGGAACGAATTACGTGCAAGCATGAGACAGAAAATGGCTGAAAGGAAGAAACTGTTGCAAACAAAGAAAGGTATGCTAGACTCATTCAACAACCCGGATCACCCCCTCTATAAATTTCGCAAACAAGTGCTCGACGAGCTCAAGGAAATGAGTGCATACGACAACACCTTGGAGGCATCCGAGATTGCGGACCGTACTTGGCTGTGGTTGGAGATCAAAAAAGCCAAAGGTACGAATGCAGTGGTAAACAGTGGAGCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGGTATGAATCTTATCAAACCGTTCGCGTGCCGGGTTACAATGAAGATATGACATCCATTGCAATGAAGAAGTTGTTCGTTGAGAGCAGTCTGACTGACGAGCAGCTGGGCGCCTTGTGCAAGCGTATATCCATTGCTAGCGATCAAGAGAACCATCTTCTGACACAGAAGACGCAGGAGATTGAGAGCACTTTCAAACAATTTTCAGATGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTGAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTGGCCGGCCTGCACGCGAATGTGGAAGCCATTGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGTTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCGGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGAGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGCGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACAGGTGGCAAGATGCCGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAGAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGATTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTAGACGTATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCGGTCCAAATGATTATAGATTGGTTCAACCGGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAACCTTGTAAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGAGACATCGCTGTTCACATTTGGGAAGGCGTTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATCGGCACACAACTAGCCTTTGATTCCGCAAGCTTTCTAGCGGGTATTGGTGCAATTGTTGCTGGAGCATCGGGCGTGGGCATGATTTTGGGCGGAGCTGGCGTGGTATTGGGAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGGAAATCACAGCAGACGCCCAACTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACCGCGGTGGCGGATACAGATACGAATCTAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACATCTCCAAGTTCATTTCGACAGCCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGCTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATTGGGTATAAGAACGATAATGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTCGCTACTTCCCGCCACGATACGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATCAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTATATGTCTTAACCAGGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCAAATAAAACATTATGGATACTTGATGCCCGACAACTCATTGCAGGCAACGATTCAATCACGGTCTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTACAGATCGTCATGAGTCGGGAGGAGCTTCTCCAAGTGGACTTGGCGTCTGGAGAAGCCCGCGTGTTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACACAGGTTGCATGGCCAGTTTGTAGTGGTGAACAATTATTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCACGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGTTACTGGAGGACCCTGACAGCAACATGGCAAGAGTTGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACCCGGACAAGGGAGTAAATGGAATCATCTATCTCGTTCATCAAGAAAGCATCGTGCTTACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGTACAATTAGCTTAACAGAATTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAGATTACCCGGCCAAAAGGTTGTCGCTACCAACGCTGAGTTTGTGACACTTCTGGCTAAGGATGAGCAAGGAGCCAATATCAAGGAGCAGACGGTCTATTGCCAACAAGGTACGGGCCAGACCAACGATACATCTATTGAAAGCATCCCGATTCCAGGGCTCTCTAATCTTTTAACCGTGTACGGTGAAGTATTCGCTACAACTAATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATGTCTCGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAGTTGCAGTATTCGGTGTTAGAGACCTACAAAATGCAGTGGTGCCAATTTGGTACCACAACGGAAAGATCGCGGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTAACAGCAACGGTGAAGCTTGGTTGTTCGACTTGGACGACGGCATTCTGTATAGCCAGCCGCTTGTGGATAGCTTTCTGTATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAATCTTTATTCCACAAGTGGAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGTTTGCATGTGACTACCATAGACGGTGTTGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAGGCCTGGCAGATATCTCGTCAGAACCGATTAGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCATCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAGCACATCCCGACGCCGTGTGTATGTTGTGGAGAGCAAAAACATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGAGGAAATAACGATTCCGACGCTGGCTGGCATCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATAGACCGAGACAATTGGAAGCATTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTGCCAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTTGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAGacgcgtCTTAATTAACTAGTGAGCTCGAATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGG

实施例3：转基因棉花株系获得(见图3)

将实施例2所得植物过表达载体VM062-Suit送江苏未米生物科技有限公司进行转化，转化背景为棉花品种“百棉1号”。从公司获得的T0代转基因棉花株系，栽培收获T1代，SEQ ID NO.19：以正向引物：5’-GACTACAAGGACGACGATGATAAGG-3’；反向引物：5’-GTATGCACTCATTTCCTTGAGCTCG-3’为引物，对苏铁毒蛋白基因基因特异1400bp片段进行PCR扩增，筛选T1阳性植株并收获种子。结果如图4所示，确定目的基因未发生遗传分离丢失，最终获得含有苏铁毒蛋白基因稳定遗传的阳性纯合转基因棉花。

实施例4：转基因棉花植株的苏铁毒蛋白基因蛋白水平表达检测

取转基因棉花纯合株系叶片，提取叶片总蛋白，用于Western Blot检测。具体步骤：

1、取新鲜样品1～2g；

2、把样品放在研钵中用液氮研磨，研磨后置于10mL试管中，并加入蛋白提取液(0.01M PBS pH＝8，1mM PMSF，2mM DTT)4℃摇匀30min。

3、12000rpm 4℃离心20min。

4、提取上清液，样品制备完成。

5、取上清加入Loading buffer，然后98℃煮5～10分钟，后用SDS-聚丙烯酰胺凝胶电泳法测定蛋白质表达情况，见图5。

实施例5：苏铁毒蛋白转基因棉花植株的抗虫性检测

取龄期一致的三龄第二天的小菜蛾(平均体重1.33mg/头)。每头注射2.03μg苏铁蛋白，对照组注射等体积(207nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果见表1。

取龄期一致的二龄第二天的棉铃虫(平均体重是2.06mg/头)。每头注射3.38μg苏铁蛋白，对照组注射等体积(345nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果见表1。

表1 注射苏铁蛋白后小菜蛾和棉铃虫幼虫的死亡率

取龄期一致的二龄草地贪夜蛾(平均体重1.01mg/头)用于草地贪夜蛾的取食实验。实验前，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫。25℃培养4天统计死亡率，取食结果见图6，死亡率统计见表2。

取龄期一致的二龄棉铃虫(平均体重3.9mg/头)用于棉铃虫的取食实验。实验前，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫。25℃培养4天统计死亡率，取食结果见图6，死亡率统计见表2。

表2 草地贪夜蛾和棉铃虫幼虫死亡率

二、应用实施例。为了证明本发明的技术方案的创造性和技术价值，该部分是对权利要求技术方案进行具体产品上或相关技术上的应用实施例。

本发明提供了一种来源于苏铁的毒蛋白基因，该基因长6240bp，编码2080个氨基酸，将其导入到棉花中，使转基因棉花获得草地贪夜蛾和棉铃虫抗性，且后代能够稳定遗传。详细实施例见：

实施例1.克隆苏铁毒蛋白基因。

实施例2.35s启动子+苏铁毒蛋白基因的植物表达载体的构建。

实施例3.转基因棉花株系获得。

实施例4.转基因棉花植株的苏铁毒蛋白基因蛋白水平表达检测。

实施例5.苏铁毒蛋白转基因棉花植株的抗虫性检测。

三、实施例相关效果的证据。本发明实施例在研发或者使用过程中取得了一些积极效果，和现有技术相比的确具备很大的优势，下面内容结合试验过程的数据、图表等进行描述。

本发明提供了来源于苏铁的毒蛋白基因，该基因是本发明首次发现，来源于苏铁的一种抗虫基因，将其导入到棉花中，根据苏铁毒蛋白基因的表达情况(图4)，说明该基因已成功导入棉花，并能稳定遗传；根据苏铁毒蛋白基因的表达情况(图5)，说明该基因所编码的蛋白已在棉花中成功表达；根据转基因植株的抗虫实验结果(图6，表1，表2)，发现转基因棉花对草地贪夜蛾抗性达到61.11％，对棉铃虫抗性达59.26％。这些结果与其它研究结果相比，说明本发明单价转基因棉花所获得的抗性与他人研究的双价转基因棉花具有类似效应。

以上所述，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，都应涵盖在本发明的保护范围之内。

序列表

<110> 河南大学

深圳华大生命科学研究院

深圳市仙湖植物园（深圳市园林研究中心）

<120> 一种抗虫性的苏铁毒蛋白基因、表达载体及应用

<160> 19

<170> SIPOSequenceListing 1.0

<210> 1

<211> 6240

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1

atggttatgg taattgacta tccaagtctc agagcttcgt tatatgccac taagagtgcg 60

gtcccatgtc gcccgcttcc gggcaatcca gagcgggtgt cgcgccgggt atcacgcaga 120

ggcgcgggag agatcgggtc ccatgcagat ttctggatct cccgcgccaa ggcatccgcc 180

atccggattt tcccgcgtca agagggtccg ctccgtgaat cccggagaag attctatctt 240

ctccgcgaag cggtgttcac cggccgcagg accgcctggg ccgctaatcc agcggcccaa 300

cgggcagcat gggaccgcaa gagatcccct ttcttcggaa ggtcttccga tgatattcat 360

tcattcagca agaatccaga agaattcttg actcaaaaca ctctcagctg cagtttatta 420

actcgcaccg ctcagaccgt cacaaaaagc ccagtattgc ccgcccaagg ttgggtcaaa 480

gtgggtcgtg tgccaggaag aggagacgct ctctttgttg agctggttgc cgagaaatca 540

gatgagacag tgaaggcgta cttacttggc tacaatggta agaacgactc ccccgagccg 600

gcatacgttg atattccaaa gaggggcgcc gagaacggaa catttttgtt taccgtggat 660

ctctcgggct gctccgttgt tgtgacacag ttggacgagc agacataccg tgttttccac 720

gacaaacggg tgaacagctc cattctgtac gacaacgtcg taatgtatgt ggatttcaat 780

cactaccgct atcccagagc ccacggacac gggagagata cgggacaagc ggtcgtctgt 840

atgcaattcg ccgacggagt gtggaaattg tttctccagc ggcacgagac ggcgaattct 900

ctcccaaaga tatggtcccg tggcgatggc aaaccggaga ttttcagcga cgccgaaccc 960

cgtccacata tggaagatgg cataaaggaa tttctcaatc ggcgtcgagc tttgcaagac 1020

gaattgaagc acttcgccaa cacgcttgaa gtggacccgg acatagtgtc caacgctgtc 1080

gacggcgaat acgaaggcgg ccagttttcc aggaacgacg atgcaattcg tggatggaac 1140

gaattacgtg caagcatgag acagaaaatg gctgaaagga agaaactgtt gcaaacaaag 1200

aaaggtatgc tagactcatt caacaacccg gatcaccccc tctataaatt tcgcaaacaa 1260

gtgctcgacg agctcaagga aatgagtgca tacgacaaca ccttggaggc atccgagatt 1320

gcggaccgta cttggctgtg gttggagatc aaaaaagcca aaggtacgaa tgcagtggta 1380

aacagtggag cagccccgaa gcagccgagt cttaccgttg gtgaacgcta cgcagagatg 1440

gaggcggccc tttggatgcg tagcgacaca gcggcgtttg agcaagggta tgaatcttat 1500

caaaccgttc gcgtgccggg ttacaatgaa gatatgacat ccattgcaat gaagaagttg 1560

ttcgttgaga gcagtctgac tgacgagcag ctgggcgcct tgtgcaagcg tatatccatt 1620

gctagcgatc aagagaacca tcttctgaca cagaagacgc aggagattga gagcactttc 1680

aaacaatttt cagatggcag ctttttggag tcggcgcctc aggatttctt cctgagacga 1740

gtcggtgata aatctggagg acgctgctat cctcttgtaa gggtaatggc cgtcgcactg 1800

tctgaaggag aagacgttgc ccagaatttt atggagaacc tgaggacatt gtctaggaat 1860

ccagagtatt caggcgaatc ggcaagttca caagccacaa atgcagggtt gttcagagat 1920

gccctggccg gcctgcacgc gaatgtggaa gccattgccg catctgagaa actacctgct 1980

gtgtttggac tgcccgatgt tgctacaatg ttgagccaag cgagcgaaag caaggtttac 2040

gctttgaata ccgaaacaca cagcatgctg gttgcggtga ttgtagacaa acagagtctg 2100

gcgcgcaagt attacttcta tgatcccaac tttctcatgg ccggtttctc cgacataact 2160

cgtttctcgg aagccctcaa gtatcacttt gtgaatctcc agttcgccgg aaactacaaa 2220

gcgtttggca cagcggaggc tccccagttt aacctggtcc ttattgatac gaccgccatg 2280

gaggatgcgc atgttgacgg agggatcacc gtgaaacaat tgagcgaggg cgttgatctt 2340

actaatgtct ttgacagcac caaaaaggct cgtgtggtaa ctgaggcagc agctcgcttg 2400

gctgaagacc aacaactgcg cactggcctc accataatag aggctgacca gtgggcaaac 2460

aggtggcaag atgccgtcat acgattgaaa ctcaaggaag gattaagtgg agagtggatg 2520

ccattgctgg agaaaatgca agaaaacgca gatggttctt atcagattga tttcatcaac 2580

accaagaata ctggggaaat ccgtggggtt tccactagag atgcgacgtt caaagaattc 2640

aaatcctacc tggataaaaa tttagacgta ttgcataagg atttcatctt tcaggatgga 2700

gttttcaaac cactggaggg agagcccatt gacgggttga atgcaatgtt cgcggtccaa 2760

atgattatag attggttcaa ccgggggact aatgctgagg aagatgcggc ctcaggatcc 2820

accaatgccc aactttccct agcgattaaa gtgcacagct acattaacat ggcccaagta 2880

gcgcatactg cagttacaga tgttgccaag gtatacaacc ttgtaaagtc agcactgtat 2940

ggcgagcagg tagctgggga gacatcgctg ttcacatttg ggaaggcgtt gaaatttgca 3000

gcaacagaag ggttgggagt cttactaggg gcgtcctttg tgatcttgga tgcctaccag 3060

ctgtccctcg tggaaaacga tgtgcagaag gccattatcg gcacacaact agcctttgat 3120

tccgcaagct ttctagcggg tattggtgca attgttgctg gagcatcggg cgtgggcatg 3180

attttgggcg gagctggcgt ggtattggga ggcctgggag ttggctttgg agccttagct 3240

caggcattcg gggaaatcac agcagacgcc caactggtgg gcaagtactt cgcactcttg 3300

gacgaggcat accgcggtgg cggatacaga tacgaatcta aagacaaggc attgatgcct 3360

cttgcaggag cagtcatcga caaaatacga ctggccgaca aacatctcca agttcatttc 3420

gacagccaat tcatatatcg aacccatcat ggcagcacag gatccggcaa aattaattac 3480

ttcttctggg ctggcgacat gccgcagatg gtccacgatc gctcccaagc tatcaatgtt 3540

cgagagcaaa ttgggtataa gaacgataat gctactttgg agctaccgga ggatgcctct 3600

gatctgatcc tgcctgccac gcccaagtca tttatcactt accgatacga gattctacca 3660

ttcgctactt cccgccacga tacgggtttc gatgtcatta gacgcctgga agaagactac 3720

agattcgatt acgatttcta catcttccca tctgaataca caatcagaac catcaaacac 3780

gagtacgtag aaacaaaggt ggcagtagtc ctggataggc gctctttgcg actgggagca 3840

cctgagcttc tcaaggagca acatggctat ttgatctatg agatcgaggg gaccggagga 3900

caatacgtta tatgtcttaa ccagggatcc actgtcacac tgaagaccac cggatcagag 3960

gcaaataaaa cattatggat acttgatgcc cgacaactca ttgcaggcaa cgattcaatc 4020

acggtctctc aagatagcat caccatcggc agcacaacag tccatctccg taaccaagat 4080

ttcgattgcc tacagatcgt catgagtcgg gaggagcttc tccaagtgga cttggcgtct 4140

ggagaagccc gcgtgttttc agaagatgcc agtaaatggc tcgacaccaa agaaacaatc 4200

catgaacatc tccataagtt ggctgaagaa cacaggttgc atggccagtt tgtagtggtg 4260

aacaattatt atcgtggagt gggccgtgcc tactatgatg ctgctcacga tcggatgttg 4320

tatacagatg tggaagatga aactctgact cgggatgctg ttcttgccgg cgttacaggc 4380

gaagatgcct acttctacaa tgtaaagcag aatgccctgt ggcgagtgaa tgcaggcaca 4440

caccaagtaa taaccaaata ctatccgacg ttactggagg accctgacag caacatggca 4500

agagttgagc agcatggcaa catcgtcgtg atggccatga gcaggctcaa ccacccggac 4560

aagggagtaa atggaatcat ctatctcgtt catcaagaaa gcatcgtgct tactgctgtg 4620

atcggcaatt cagagatgct caaacgtctt cgccaaacga gtacaattag cttaacagaa 4680

ttgcgaggtg tcaatgacat ggctctggtt tccacgatgg acagattacc cggccaaaag 4740

gttgtcgcta ccaacgctga gtttgtgaca cttctggcta aggatgagca aggagccaat 4800

atcaaggagc agacggtcta ttgccaacaa ggtacgggcc agaccaacga tacatctatt 4860

gaaagcatcc cgattccagg gctctctaat cttttaaccg tgtacggtga agtattcgct 4920

acaactaatg atggtcgcat ccttcgcctg cttggggacg gctcaacatg tctcgagggc 4980

atcaatgagc attggctcat caagcatcca aaatggtggc aggacttggc taccttggag 5040

gcagagaaag ccacgacagt tgcagtattc ggtgttagag acctacaaaa tgcagtggtg 5100

ccaatttggt accacaacgg aaagatcgcg gtggcctcat caaaactgta tggcaaacaa 5160

ctgcaatttt tggggtttaa cagcaacggt gaagcttggt tgttcgactt ggacgacggc 5220

attctgtata gccagccgct tgtggatagc tttctgtatg caatctttag tttggacggg 5280

aagcttattg caccaatctt tattccacaa gtggagcaaa tgctcagcgg ccagcatctc 5340

agaaaccctg tcatcgtgga cggccgtttg catgtgacta ccatagacgg tgttgttgtt 5400

ggatttgaca agaagtcgcc aactctggtt gcagtgaaca aggcctggca gatatctcgt 5460

cagaaccgat tagaagaggc cctcggaaac ctgctgtcca attggtctga tcacaatgat 5520

gcgattgtgt tgcagggtga tccagaatca gcgccggcat ggtattatgt tccatcaaga 5580

gaaatagtga ccgccaatgg tctaacatgg gcggatcatc ctctatggct aggaatggct 5640

gtcggtgctg caacgccata catctacagc acatcccgac gccgtgtgta tgttgtggag 5700

agcaaaaaca tggccaaaga caaaggagaa ttcgcagcag cacattgctt tgcaaaatca 5760

cttgtgctac agagcatcgt tcccgaggaa ataacgattc cgacgctggc tggcatcgat 5820

ttcgcactcg ttgcagcgac aaatgcaacc attgtaatag accgagacaa ttggaagcat 5880

tacaaggcca ttgtgctcga caacaaggtg gatcaggacg gtggcgctaa ggtggtagtc 5940

ggcagtgaga ttcccatgaa agttctggtg cgcaaatttc agggaaatct ggagttcttc 6000

gatccaaaca atggtaatgt tgttatactc cagaatgtgt tgaagggaga cggagcttgt 6060

tgccagatag agatcaaagt aggcgatgct gagactatgc ttgtatccga tttctcggag 6120

atattctcaa agacgcatcc tctggtggca agccaagtat ttgagcttgg ggatgtgatt 6180

gcagtcacaa aacacaaatc ggactatgcg gatctgtggg ccattctacg catgacatag 6240

<210> 2

<211> 5985

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 2

<210> 3

<211> 4950

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

<210> 4

<211> 5862

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

<210> 5

<211> 6087

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 5

<210> 6

<211> 6240

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 6

atggtgatgg ttattgatta cccttctttg agggcttctt tgtatgctac taagtctgct 60

gttccttgta ggcctcttcc tggtaaccct gaaagggttt ctaggagggt ttctagaagg 120

ggtgctggtg aaattggtag tcatgctgat ttttggattt ctagagctaa ggcttctgct 180

attaggattt ttcctagaca agaaggtcca ttgagggaaa gcaggaggag gttctatttg 240

ttgagagagg ctgttttcac aggtaggagg actgcatggg ctgcaaatcc tgctgctcag 300

agagcagctt gggatagaaa gaggtctcct ttcttcggta ggtcatcaga tgatattcat 360

tcattcagta agaacccaga agagttcctt actcaaaata ctttgtcttg ttctcttctt 420

actagaactg ctcaaactgt tactaagtct cctgttttgc ctgctcaagg ttgggttaag 480

gttggtagag tgccaggtag aggtgatgct cttttcgttg agcttgttgc tgaaaaatct 540

gatgaaactg ttaaggctta cttgttgggt tataatggta agaatgattc tcctgaacct 600

gcttatgttg atattccaaa gaggggtgca gagaatggaa ctttcctttt tacagtggat 660

cttagcggtt gttctgttgt tgttactcaa ttggatgagc agacttatag agtgttccat 720

gataagaggg ttaattcaag tattctttac gataacgtgg ttatgtatgt tgattttaac 780

cattacaggt accctagagc tcatggtcat ggtagagata ctggtcaagc tgttgtttgt 840

atgcagtttg ctgatggtgt ttggaagttg ttcttgcaaa gacatgagac tgctaattct 900

cttccaaaga tttggtctag aggtgatggt aagcctgaaa ttttctctga tgctgagcct 960

agacctcata tggaggatgg tattaaggag ttccttaata gaaggagggc tttgcaagat 1020

gaattgaagc attttgctaa tactttggaa gttgatcctg atattgtttc taatgctgtt 1080

gatggtgaat atgaaggtgg tcaattttct agaaatgatg atgcaattag gggttggaat 1140

gaattgagag cttctatgag acagaagatg gctgaaagaa agaagcttct tcaaactaag 1200

aagggtatgt tggatagttt caataatcca gatcatcctc tttacaagtt cagaaaacaa 1260

gttttggatg aattgaaaga gatgtcagct tatgataata cacttgaagc ttctgaaatc 1320

gctgatagga cttggctttg gcttgaaatt aagaaggcta agggaacaaa tgcagttgtt 1380

aatagcggag ctgcacctaa gcaaccatcc cttactgttg gtgaaagata tgctgaaatg 1440

gaagcagcac tttggatgag aagcgataca gctgctttcg aacaaggata tgaaagttat 1500

caaactgtta gagttccagg ttataatgaa gatatgacaa gcattgctat gaagaagttg 1560

tttgttgaat cttccttgac agatgaacaa cttggtgctc tttgtaagag gatttccatt 1620

gctagtgatc aagaaaatca tttgttgact caaaagaccc aagaaatcga atcaactttc 1680

aagcaattct ccgatggaag cttccttgaa tctgctccac aagatttctt ccttagaaga 1740

gttggtgata aatccggtgg taggtgctac ccacttgtta gggttatggc tgttgctctt 1800

tctgaaggtg aagatgttgc tcaaaatttc atggaaaatt tgaggacttt gtcaaggaat 1860

cctgaatatt ccggtgaatc agcttcttct caagctacaa atgctggtct tttcagagat 1920

gctcttgctg gtcttcatgc aaacgttgaa gctatcgctg catccgagaa gcttcctgct 1980

gtttttggtt tgcctgatgt tgctactatg ctttctcagg cttctgaatc taaggtttat 2040

gctcttaata ctgaaactca ttctatgctt gttgctgtta ttgttgataa gcaatctttg 2100

gctaggaagt attattttta cgatcctaat ttccttatgg ctggtttctc tgatattact 2160

agattctccg aagctcttaa gtaccatttc gttaatttgc agtttgcagg taattataag 2220

gctttcggta ctgctgaagc tcctcaattc aatcttgttc ttattgatac taccgctatg 2280

gaggatgctc atgttgatgg tggtattact gtgaagcaac tttctgaagg agttgatctt 2340

actaatgttt tcgattctac taagaaggct agagtggtta ctgaggctgc agctaggttg 2400

gctgaagatc agcaattgag aactggactt acaattattg aagctgatca gtgggctaat 2460

agatggcagg atgctgttat taggttgaag cttaaggaag gtctttctgg tgagtggatg 2520

cctttgcttg aaaagatgca agaaaatgca gatggtagtt atcagattga tttcattaac 2580

actaagaaca ctggagaaat taggggagtt tcaactaggg atgctacttt taaggaattc 2640

aagtcttatt tggataagaa ccttgatgtg ttgcataagg attttatttt ccaggatgga 2700

gtgttcaaac ctcttgaggg tgagcctatt gatggactta atgctatgtt cgctgttcaa 2760

atgattattg attggttcaa taggggtact aacgcagaag aagatgctgc ttctggttct 2820

actaacgctc aattgtcttt ggctattaag gttcattctt atatcaacat ggcacaagtg 2880

gcacatactg ctgttactga tgtggcaaag gtttataatt tggtgaaatc tgctctttac 2940

ggtgaacaag ttgctggtga aacatcattg ttcactttcg gtaaggctct taaatttgct 3000

gctactgaag gtcttggtgt gttgcttggt gcttcttttg tgatccttga tgcttatcag 3060

ctttctcttg ttgagaatga tgttcagaag gcaattattg gtactcaact tgcttttgat 3120

tctgcttctt ttcttgctgg tattggtgct attgttgctg gtgcttctgg tgttggtatg 3180

attttgggtg gtgctggtgt tgttttgggt ggtttgggtg ttggttttgg tgctttggct 3240

caggcttttg gtgagattac tgctgatgct cagttggttg gtaagtattt tgctttgttg 3300

gatgaggctt ataggggtgg tggttatagg tatgagtcta aggataaggc tttgatgcct 3360

ttggctggtg ctgtgattga taagattaga cttgctgata agcatttgca agttcatttt 3420

gattctcaat tcatctacag gactcatcat ggttctactg gttctggtaa gattaattat 3480

ttcttctggg ctggtgatat gcctcaaatg gttcatgata gatctcaagc tattaatgtt 3540

agggagcaga ttggttataa gaatgataat gctactcttg aacttccaga ggatgcttct 3600

gatcttattc ttcctgcaac tcctaagtct ttcattacat atagatacga gatcttgcca 3660

ttcgctactt ctagacatga tactggtttt gatgttatta gaagacttga ggaggattat 3720

agattcgatt atgatttcta catcttccct tctgaatata ccattagaac tattaagcac 3780

gaatacgttg aaaccaaggt tgctgttgtg cttgatagaa gatctttgag acttggtgct 3840

cctgagcttt tgaaggaaca acatggttat cttatttacg agattgaagg tacaggtggt 3900

caatatgtta tttgtttgaa tcagggttct actgtgactc ttaagacaac tggttctgaa 3960

gcaaataaga ctctttggat tttggatgct aggcaactta ttgctggtaa tgatagtatc 4020

actgtttctc aagattctat tactatcggt tctactactg tgcatcttag gaatcaggat 4080

ttcgattgtc ttcagattgt tatgtctaga gaagaacttc ttcaagttga tttggcttct 4140

ggtgaagcta gagttttctc cgaagatgct tctaagtggc ttgatacaaa ggaaactatt 4200

catgaacacc ttcacaagtt ggctgaagaa cataggcttc atggtcaatt cgttgttgtt 4260

aataattact acaggggtgt tggtagagct tattatgatg ctgctcatga tagaatgctt 4320

tatactgatg ttgaggatga aactcttaca agagatgctg ttcttgctgg tgttactggt 4380

gaagatgctt atttttataa cgttaagcag aacgctttgt ggagagttaa cgctggtact 4440

caccaagtta ttactaagta ttatcctact cttcttgagg atcctgattc taacatggct 4500

agagttgagc aacatggtaa tatcgttgtt atggctatgt caagattgaa tcatcctgat 4560

aagggtgtta acggtattat ttatcttgtt catcaggaat ctatcgttct tactgctgtt 4620

attggaaact ctgaaatgct taagagactt agacaaactt ctactatctc tcttactgaa 4680

cttaggggtg ttaacgatat ggctcttgtt tcaactatgg ataggcttcc tggtcagaag 4740

gtggtggcta caaatgcaga attcgttaca cttttggcta aggatgaaca aggagctaat 4800

attaaggaac aaactgttta ttgccagcaa ggtactggtc aaactaatga tacttctatt 4860

gaatcaatcc ctatccctgg tttgtctaat cttcttacag tttatggtga ggttttcgct 4920

acaactaatg atggtagaat ccttagattg cttggtgatg gaagcacttg tcttgagggt 4980

atcaatgaac attggttgat taagcatcct aaatggtggc aggatcttgc tactcttgag 5040

gcagagaagg ctaccactgt tgctgttttt ggagttaggg atttgcaaaa tgctgttgtt 5100

cctatttggt atcataacgg taagattgca gttgcttcat ctaaacttta tggtaagcaa 5160

ttgcagtttt tgggttttaa ttctaacgga gaagcttggc ttttcgatct tgatgatggt 5220

attttgtatt ctcaaccttt ggttgattct ttcctttatg ctattttcag tttggatggt 5280

aagttgattg ctcctatctt tattcctcag gttgaacaaa tgttgtccgg tcaacatctt 5340

aggaaccctg ttattgttga tggtaggctt catgttacta ctattgatgg tgttgttgtt 5400

ggtttcgata agaagtctcc tactcttgtg gctgttaata aggcttggca aatttctaga 5460

caaaataggc ttgaggaggc tttgggtaat ttgttgtcta attggtctga tcataacgat 5520

gctattgttc ttcagggtga tcctgagtct gctcctgctt ggtattatgt tccttctagg 5580

gagattgtga ctgctaatgg tttgacttgg gctgatcacc ctctttggct tggaatggct 5640

gttggtgctg ctactcctta tatttattct acttctagaa ggagggttta tgttgttgag 5700

tctaagaata tggctaagga taagggtgaa tttgctgctg ctcattgttt tgctaagtct 5760

ttggttcttc agtctattgt tcctgaggaa attactattc caactcttgc tggtatcgat 5820

ttcgctttgg ttgctgctac taatgctact attgttattg atagggataa ctggaagcat 5880

tataaggcaa ttgttcttga taacaaggtt gatcaagatg gaggtgctaa ggttgtggtt 5940

ggttctgaga ttcctatgaa ggttcttgtg aggaagtttc aaggtaattt ggaatttttc 6000

gatcctaata acggtaatgt ggttattctt caaaacgttc ttaagggaga tggtgcttgt 6060

tgtcagattg agattaaggt tggtgatgca gagactatgt tggtttctga tttctctgag 6120

attttctcta agactcatcc tttggttgct tctcaggttt tcgaacttgg tgatgttatt 6180

gctgttacta agcataagag tgattatgct gatctttggg ctattttgag gatgacttga 6240

<210> 7

<211> 5985

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 7

<210> 8

<211> 4590

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 8

<210> 9

<211> 5862

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 9

<210> 10

<211> 6087

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 10

<210> 11

<211> 2079

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 11

Met Val Met Val Ile Asp Tyr Pro Ser Leu Arg Ala Ser Leu Tyr Ala

1 5 10 15

Thr Lys Ser Ala Val Pro Cys Arg Pro Leu Pro Gly Asn Pro Glu Arg

20 25 30

Val Ser Arg Arg Val Ser Arg Arg Gly Ala Gly Glu Ile Gly Ser His

35 40 45

Ala Asp Phe Trp Ile Ser Arg Ala Lys Ala Ser Ala Ile Arg Ile Phe

50 55 60

Pro Arg Gln Glu Gly Pro Leu Arg Glu Ser Arg Arg Arg Phe Tyr Leu

65 70 75 80

Leu Arg Glu Ala Val Phe Thr Gly Arg Arg Thr Ala Trp Ala Ala Asn

85 90 95

Pro Ala Ala Gln Arg Ala Ala Trp Asp Arg Lys Arg Ser Pro Phe Phe

100 105 110

Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu

115 120 125

Phe Leu Thr Gln Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr Ala

130 135 140

Gln Thr Val Thr Lys Ser Pro Val Leu Pro Ala Gln Gly Trp Val Lys

145 150 155 160

Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu Phe Val Glu Leu Val

165 170 175

Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr Leu Leu Gly Tyr Asn

180 185 190

Gly Lys Asn Asp Ser Pro Glu Pro Ala Tyr Val Asp Ile Pro Lys Arg

195 200 205

Gly Ala Glu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys

210 215 220

Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His

225 230 235 240

Asp Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met Tyr

245 250 255

Val Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly Arg

260 265 270

Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val Trp

275 280 285

Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile

290 295 300

Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro

305 310 315 320

Arg Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg

325 330 335

Ala Leu Gln Asp Glu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp

340 345 350

Pro Asp Ile Val Ser Asn Ala Val Asp Gly Glu Tyr Glu Gly Gly Gln

355 360 365

Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg Ala

370 375 380

Ser Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu Leu Gln Thr Lys

385 390 395 400

Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr Lys

405 410 415

Phe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp

420 425 430

Asn Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu

435 440 445

Glu Ile Lys Lys Ala Lys Gly Thr Asn Ala Val Val Asn Ser Gly Ala

450 455 460

Ala Pro Lys Gln Pro Ser Leu Thr Val Gly Glu Arg Tyr Ala Glu Met

465 470 475 480

Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu Gln Gly

485 490 495

Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu Asp Met

500 505 510

Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp

515 520 525

Glu Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln

530 535 540

Glu Asn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe

545 550 555 560

Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp Phe

565 570 575

Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro Leu

580 585 590

Val Arg Val Met Ala Val Ala Leu Ser Glu Gly Glu Asp Val Ala Gln

595 600 605

Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser

610 615 620

Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp

625 630 635 640

Ala Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu

645 650 655

Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser

660 665 670

Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His Ser

675 680 685

Met Leu Val Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr

690 695 700

Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr

705 710 715 720

Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala

725 730 735

Gly Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu

740 745 750

Val Leu Ile Asp Thr Thr Ala Met Glu Asp Ala His Val Asp Gly Gly

755 760 765

Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val Phe

770 775 780

Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg Leu

785 790 795 800

Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp

805 810 815

Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys

820 825 830

Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu

835 840 845

Asn Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr

850 855 860

Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe

865 870 875 880

Lys Ser Tyr Leu Asp Lys Asn Leu Asp Val Leu His Lys Asp Phe Ile

885 890 895

Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly

900 905 910

Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg

915 920 925

Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln

930 935 940

Leu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val

945 950 955 960

Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys

965 970 975

Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Glu Thr Ser Leu Phe Thr

980 985 990

Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu

995 1000 1005

Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val

1010 1015 1020

Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp

1025 1030 1035 1040

Ser Ala Ser Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser

1045 1050 1055

Gly Val Gly Met Ile Leu Gly Gly Ala Gly Val Val Leu Gly Gly Leu

1060 1065 1070

Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Glu Ile Thr Ala

1075 1080 1085

Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala Tyr

1090 1095 1100

Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro

1105 1110 1115 1120

Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu

1125 1130 1135

Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Ser

1140 1145 1150

Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro

1155 1160 1165

Gln Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln Ile

1170 1175 1180

Gly Tyr Lys Asn Asp Asn Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser

1185 1190 1195 1200

Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr

1205 1210 1215

Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Thr Gly Phe Asp Val

1220 1225 1230

Ile Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile

1235 1240 1245

Phe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu

1250 1255 1260

Thr Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala

1265 1270 1275 1280

Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile Glu

1285 1290 1295

Gly Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn Gln Gly Ser Thr Val

1300 1305 1310

Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu

1315 1320 1325

Asp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln

1330 1335 1340

Asp Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp

1345 1350 1355 1360

Phe Asp Cys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln Val

1365 1370 1375

Asp Leu Ala Ser Gly Glu Ala Arg Val Phe Ser Glu Asp Ala Ser Lys

1380 1385 1390

Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala

1395 1400 1405

Glu Glu His Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr

1410 1415 1420

Arg Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu

1425 1430 1435 1440

Tyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala

1445 1450 1455

Gly Val Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala

1460 1465 1470

Leu Trp Arg Val Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr

1475 1480 1485

Pro Thr Leu Leu Glu Asp Pro Asp Ser Asn Met Ala Arg Val Glu Gln

1490 1495 1500

His Gly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp

1505 1510 1515 1520

Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Ile Val

1525 1530 1535

Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln

1540 1545 1550

Thr Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala

1555 1560 1565

Leu Val Ser Thr Met Asp Arg Leu Pro Gly Gln Lys Val Val Ala Thr

1570 1575 1580

Asn Ala Glu Phe Val Thr Leu Leu Ala Lys Asp Glu Gln Gly Ala Asn

1585 1590 1595 1600

Ile Lys Glu Gln Thr Val Tyr Cys Gln Gln Gly Thr Gly Gln Thr Asn

1605 1610 1615

Asp Thr Ser Ile Glu Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu Leu

1620 1625 1630

Thr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly Arg Ile Leu

1635 1640 1645

Arg Leu Leu Gly Asp Gly Ser Thr Cys Leu Glu Gly Ile Asn Glu His

1650 1655 1660

Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala Thr Leu Glu

1665 1670 1675 1680

Ala Glu Lys Ala Thr Thr Val Ala Val Phe Gly Val Arg Asp Leu Gln

1685 1690 1695

Asn Ala Val Val Pro Ile Trp Tyr His Asn Gly Lys Ile Ala Val Ala

1700 1705 1710

Ser Ser Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe Asn Ser

1715 1720 1725

Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser

1730 1735 1740

Gln Pro Leu Val Asp Ser Phe Leu Tyr Ala Ile Phe Ser Leu Asp Gly

1745 1750 1755 1760

Lys Leu Ile Ala Pro Ile Phe Ile Pro Gln Val Glu Gln Met Leu Ser

1765 1770 1775

Gly Gln His Leu Arg Asn Pro Val Ile Val Asp Gly Arg Leu His Val

1780 1785 1790

Thr Thr Ile Asp Gly Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr

1795 1800 1805

Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu

1810 1815 1820

Glu Glu Ala Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp

1825 1830 1835 1840

Ala Ile Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr

1845 1850 1855

Val Pro Ser Arg Glu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp

1860 1865 1870

His Pro Leu Trp Leu Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile

1875 1880 1885

Tyr Ser Thr Ser Arg Arg Arg Val Tyr Val Val Glu Ser Lys Asn Met

1890 1895 1900

Ala Lys Asp Lys Gly Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser

1905 1910 1915 1920

Leu Val Leu Gln Ser Ile Val Pro Glu Glu Ile Thr Ile Pro Thr Leu

1925 1930 1935

Ala Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val

1940 1945 1950

Ile Asp Arg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn

1955 1960 1965

Lys Val Asp Gln Asp Gly Gly Ala Lys Val Val Val Gly Ser Glu Ile

1970 1975 1980

Pro Met Lys Val Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe

1985 1990 1995 2000

Asp Pro Asn Asn Gly Asn Val Val Ile Leu Gln Asn Val Leu Lys Gly

2005 2010 2015

Asp Gly Ala Cys Cys Gln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr

2020 2025 2030

Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu

2035 2040 2045

Val Ala Ser Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys

2050 2055 2060

His Lys Ser Asp Tyr Ala Asp Leu Trp Ala Ile Leu Arg Met Thr

2065 2070 2075

<210> 12

<211> 1994

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 12

Met Glu Glu Ala Asp Met Glu Asn Arg Gly Tyr Glu Glu Arg His Ser

1 5 10 15

Gln Ser Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Phe

20 25 30

Phe Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu

35 40 45

Glu Phe Leu Thr Lys Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr

50 55 60

Ala Gln Thr Val Thr Lys Ser Pro Val Leu Pro Ala Gln Gly Trp Val

65 70 75 80

Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu Ser Gly Cys Ser

85 90 95

Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp

100 105 110

Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met Tyr Val

115 120 125

Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly Arg Asp

130 135 140

Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val Trp Lys

145 150 155 160

Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile Trp

165 170 175

Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro Arg

180 185 190

Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala

195 200 205

Leu Gln Asp Glu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp Pro

210 215 220

Glu Ile Val Ser Asn Ala Val Asp Gly Glu Tyr Glu Gly Gly Gln Phe

225 230 235 240

Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg Ala Ser

245 250 255

Met Arg Gln Lys Met Ala Glu Arg Lys Lys Arg Leu Glu Arg Asn Lys

260 265 270

Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr Lys Phe

275 280 285

Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp Asn

290 295 300

Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu

305 310 315 320

Ile Lys Lys Ala Lys Gly Thr Asn Ala Val Val Asn Ser Gly Ala Ala

325 330 335

Pro Lys Gln Pro Ser Leu Thr Val Gly Glu Arg Tyr Ala Glu Met Glu

340 345 350

Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu Gln Gly Tyr

355 360 365

Glu Ser Tyr Gln Thr Leu Arg Val Pro Gly Tyr Asn Glu Asp Met Thr

370 375 380

Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp Glu

385 390 395 400

Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln Glu

405 410 415

Asn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe Lys

420 425 430

Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp Phe Phe

435 440 445

Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro Leu Val

450 455 460

Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn

465 470 475 480

Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly

485 490 495

Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala

500 505 510

Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys

515 520 525

Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser Gln

530 535 540

Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His Ser Met

545 550 555 560

Leu Val Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr Tyr

565 570 575

Phe Tyr Asp Pro Asn Phe Leu Ile Ala Gly Phe Ser Asp Ile Thr Arg

580 585 590

Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly

595 600 605

Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val

610 615 620

Leu Ile Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly Gly Ile

625 630 635 640

Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val Phe Asp

645 650 655

Ser Thr Lys Lys Ala Arg Val Val Thr Glu Thr Ala Ala Arg Leu Ala

660 665 670

Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln

675 680 685

Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu

690 695 700

Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu Asn

705 710 715 720

Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr Gly

725 730 735

Glu Ile Arg Gly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe Lys

740 745 750

Ser Tyr Leu Asp Lys Asn Leu Asp Val Leu His Lys Asp Phe Ile Phe

755 760 765

Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly Leu

770 775 780

Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg Gly

785 790 795 800

Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu

805 810 815

Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala

820 825 830

His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys Ser

835 840 845

Ala Leu Tyr Gly Glu Gln Val Ala Gly Glu Ala Ser Leu Leu Thr Phe

850 855 860

Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu Leu

865 870 875 880

Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu

885 890 895

Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser

900 905 910

Ala Ser Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly

915 920 925

Val Gly Met Ile Leu Gly Gly Ala Gly Val Val Leu Gly Gly Leu Gly

930 935 940

Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Glu Ile Thr Ala Asp

945 950 955 960

Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala Tyr Arg

965 970 975

Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu

980 985 990

Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln

995 1000 1005

Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Leu Thr

1010 1015 1020

Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro Gln

1025 1030 1035 1040

Met Val His Asp Arg Ser Gln Ala Ile Asn Ile Arg Glu Gln Ile Gly

1045 1050 1055

Tyr Lys Asn Asp His Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser Asp

1060 1065 1070

Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Gln Tyr Gln

1075 1080 1085

Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile

1090 1095 1100

Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe

1105 1110 1115 1120

Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr

1125 1130 1135

Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala Pro

1140 1145 1150

Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile Glu Gly

1155 1160 1165

Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn Gln Gly Ser Thr Val Thr

1170 1175 1180

Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp

1185 1190 1195 1200

Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp

1205 1210 1215

Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe

1220 1225 1230

Asp Cys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln Val Asp

1235 1240 1245

Leu Ala Ser Gly Glu Ala Arg Val Phe Ser Glu Asp Ala Ser Lys Trp

1250 1255 1260

Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala Glu

1265 1270 1275 1280

Glu His Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr Arg

1285 1290 1295

Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr

1300 1305 1310

Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly

1315 1320 1325

Val Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu

1330 1335 1340

Trp Arg Val Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro

1345 1350 1355 1360

Thr Leu Leu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His

1365 1370 1375

Gly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp Lys

1380 1385 1390

Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu

1395 1400 1405

Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr

1410 1415 1420

Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu

1425 1430 1435 1440

Val Ser Thr Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn

1445 1450 1455

Ala Glu Phe Val Thr Ile Leu Ala Lys Asp Glu Gln Gly Ser Gln Val

1460 1465 1470

Cys Phe Trp Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile

1475 1480 1485

Gln Pro Pro Ile Pro Thr Asp Leu Thr Leu Thr Val Ser Ser His Lys

1490 1495 1500

Val Ala Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys Glu Gln Thr

1505 1510 1515 1520

Val Tyr Cys Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Ile Glu

1525 1530 1535

Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu Leu Thr Val Tyr Gly Glu

1540 1545 1550

Val Phe Ala Thr Thr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp

1555 1560 1565

Gly Ser Thr Tyr Leu Asp Gly Ile Asn Glu His Trp Leu Ile Lys His

1570 1575 1580

Pro Lys Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr

1585 1590 1595 1600

Thr Val Ala Val Phe Gly Val Arg Asp Leu Gln Asn Ala Val Val Pro

1605 1610 1615

Ile Trp Tyr His Asn Gly Lys Ile Ala Val Ala Ser Ser Lys Leu Tyr

1620 1625 1630

Gly Lys Gln Leu Gln Phe Leu Gly Phe Asn Ser Asn Gly Glu Ala Trp

1635 1640 1645

Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp

1650 1655 1660

Ser Phe Leu Tyr Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro

1665 1670 1675 1680

Ile Phe Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg

1685 1690 1695

Asn Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly

1700 1705 1710

Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Val Asn

1715 1720 1725

Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly

1730 1735 1740

Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln

1745 1750 1755 1760

Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu

1765 1770 1775

Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu

1780 1785 1790

Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg

1795 1800 1805

Arg Arg Val Tyr Ala Val Glu Ser Lys His Met Ala Lys Asp Lys Gly

1810 1815 1820

Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser

1825 1830 1835 1840

Ile Val Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Ile Asp Phe

1845 1850 1855

Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile His Arg Asp Asn

1860 1865 1870

Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp

1875 1880 1885

Gly Gly Ala Lys Val Val Val Gly Ser Gly Ile Pro Met Lys Val Leu

1890 1895 1900

Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly

1905 1910 1915 1920

Asn Val Val Ile Leu Arg Asn Val Leu Lys Gly Asp Gly Ala Cys Ser

1925 1930 1935

Gln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp

1940 1945 1950

Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val

1955 1960 1965

Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser Asp Tyr

1970 1975 1980

Ala Asp Leu Trp Ala Ile Leu Arg Met Thr

1985 1990

<210> 13

<211> 1529

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 13

Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn Phe Met

1 5 10 15

Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly Glu Ser

20 25 30

Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala

35 40 45

Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro

50 55 60

Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser Gln Ala Ser

65 70 75 80

Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His Ser Met Leu Ala

85 90 95

Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr Tyr Phe Tyr

100 105 110

Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe Ser

115 120 125

Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr

130 135 140

Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile

145 150 155 160

Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly Gly Ile Thr Val

165 170 175

Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val Phe Asp Ser Thr

180 185 190

Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg Leu Ala Glu Asp

195 200 205

Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln Trp Ala

210 215 220

Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu Gly Leu

225 230 235 240

Ser Gly Glu Trp Met Pro Leu Leu Glu Arg Met Gln Glu Asn Gly His

245 250 255

Gly Ser Tyr Gln Ile Glu Phe Leu Asn Thr Lys Asn Thr Gly Glu Thr

260 265 270

Arg Trp Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe Lys Ser Tyr

275 280 285

Leu Asp Lys Asn Phe Asp Ala Leu Arg Lys Asp Phe Ile Phe Gln Asp

290 295 300

Gly Val Phe Lys Pro Leu Asp Gly Glu Pro Ile Asp Gly Leu Asn Ala

305 310 315 320

Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Pro Gly Thr Asn

325 330 335

Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu

340 345 350

Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala His Thr

355 360 365

Ala Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys Ser Ala Leu

370 375 380

Tyr Gly Glu Gln Val Ala Gly Glu Ala Ser Leu Leu Thr Phe Gly Lys

385 390 395 400

Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu Leu Gly Ala

405 410 415

Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn Asp

420 425 430

Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala Ser

435 440 445

Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly Val Gly

450 455 460

Met Ile Leu Gly Gly Ala Gly Val Ile Leu Gly Gly Leu Gly Val Gly

465 470 475 480

Phe Gly Ala Leu Ala Gln Ala Phe Gly Gln Ile Thr Ala Asp Ala Gln

485 490 495

Leu Val Gly Glu Tyr Phe Ala Leu Leu Asp Glu Ala Tyr Ser Gly Gly

500 505 510

Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu Ala Gly

515 520 525

Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys Arg Leu Gln Val His

530 535 540

Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser

545 550 555 560

Gly Lys Ile Asn Tyr Phe Phe Trp Val Gly Asp Met Pro Gln Met Val

565 570 575

His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln Ile Gly Tyr Lys

580 585 590

Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser Asp Leu Ile

595 600 605

Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr Glu Ile Leu

610 615 620

Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg

625 630 635 640

Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser

645 650 655

Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr Lys Val

660 665 670

Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala Pro Glu Leu

675 680 685

Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile Glu Gly Thr Gly

690 695 700

Gly Gln Tyr Val Ile Cys Leu Asn His Gly Ser Thr Val Thr Leu Lys

705 710 715 720

Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp Ala Arg

725 730 735

Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser Ile

740 745 750

Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe Asp Cys

755 760 765

Leu Gln Ile Val Met Ser Arg Asp Glu Leu Leu Gln Val Asp Leu Ala

770 775 780

Ser Arg Glu Val Arg Val Phe Ser Glu Asp Ala Ser Lys Trp Leu Asp

785 790 795 800

Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala Gly Glu His

805 810 815

Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr Arg Gly Val

820 825 830

Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr Thr Asp

835 840 845

Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val Thr

850 855 860

Gly Glu Asp Ala Tyr Phe Tyr Asn Val Asn Gln Asn Ala Leu Trp Arg

865 870 875 880

Val Asn Ala Asp Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Leu

885 890 895

Leu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn

900 905 910

Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp Lys Gly Val

915 920 925

Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu Thr Ala

930 935 940

Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr

945 950 955 960

Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser

965 970 975

Thr Met Asp Arg Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu

980 985 990

Phe Val Thr Leu Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe

995 1000 1005

Trp Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln Pro

1010 1015 1020

Pro Ile Pro Thr Asp Leu Thr Leu Thr Val Ser Ser His Lys Val Ala

1025 1030 1035 1040

Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys Glu Gln Thr Val Tyr

1045 1050 1055

Cys Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Ile Glu Ser Ile

1060 1065 1070

Pro Ile Pro Gly Leu Ser Asn Leu Leu Thr Val Tyr Gly Glu Val Phe

1075 1080 1085

Ala Thr Thr Asn Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser

1090 1095 1100

Thr Tyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His Pro Lys

1105 1110 1115 1120

Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr Thr Val

1125 1130 1135

Ala Val Phe Gly Val Arg Asp Pro Gln Asn Ala Val Val Pro Ile Trp

1140 1145 1150

Tyr His Asn Gly Lys Ile Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys

1155 1160 1165

Gln Leu Gln Phe Leu Gly Phe Asn Ser Asn Gly Glu Ala Trp Leu Phe

1170 1175 1180

Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Ser Gln

1185 1190 1195 1200

Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro Asn

1205 1210 1215

Leu Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg Asn

1220 1225 1230

Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val

1235 1240 1245

Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Val Asn Lys

1250 1255 1260

Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly Asn

1265 1270 1275 1280

Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln Gly

1285 1290 1295

Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile

1300 1305 1310

Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly

1315 1320 1325

Met Ala Val Gly Ala Ala Thr Leu Tyr Ile Tyr Ser Thr Ser Arg Arg

1330 1335 1340

Arg Val Tyr Ala Val Glu Ser Lys Lys Met Ala Lys Asp Lys Gly Glu

1345 1350 1355 1360

Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser Ile

1365 1370 1375

Val Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Thr Asp Phe Ala

1380 1385 1390

Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile Asp Arg Asp Asn Trp

1395 1400 1405

Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp Gly

1410 1415 1420

Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu Val

1425 1430 1435 1440

Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn

1445 1450 1455

Val Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Ser Lys

1460 1465 1470

Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp Phe

1475 1480 1485

Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val Phe

1490 1495 1500

Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser Asp Tyr Ala

1505 1510 1515 1520

Asp Leu Trp Ala Ile Leu Arg Met Thr

1525

<210> 14

<211> 1953

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 14

Met Glu Glu Val Asp Met Gln Asn Arg Gly Tyr Glu Glu Trp His Ser

1 5 10 15

Gln Ser Gln Ser Ala Thr Thr Leu Arg Arg Arg Lys Arg Ser Pro Val

20 25 30

Phe Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu

35 40 45

Glu Phe Leu Thr Gln Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr

50 55 60

Ala Glu Thr Val Thr Lys Ser Pro Val Leu Pro Glu Arg Gly Trp Val

65 70 75 80

Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu Phe Val Gln Val

85 90 95

Val Ala Glu Gln Ser Tyr Asp Thr Val Lys Ala Tyr Leu Leu Gly Tyr

100 105 110

Asn Gly Glu Asn Asp Ser Pro Asn Pro Ala Tyr Val Asp Ile Pro Lys

115 120 125

Arg Ala Ala Glu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly

130 135 140

Cys Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe

145 150 155 160

His Asp Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met

165 170 175

Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly

180 185 190

Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val

195 200 205

Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys

210 215 220

Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu

225 230 235 240

Pro Arg Gln His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg

245 250 255

Arg Ala Leu Gln Asp Glu Leu Lys Lys Phe Ala Asp Met Phe Gly Val

260 265 270

Asp Pro Glu Ile Val Ser Asn Ala Val Asp Gly Lys Tyr Lys Gly Gly

275 280 285

Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg

290 295 300

Ala Ser Met Arg Gln Lys Met Ala Glu Arg Asn Lys Val Leu Leu Thr

305 310 315 320

Met Lys Leu Glu Gln Pro Arg Ser Ser Arg Val Leu Pro Leu Val Asn

325 330 335

Ala Thr Gln Arg Trp Arg Arg Pro Phe Gly Cys Val Ala Thr Gln Arg

340 345 350

Arg Leu Ser Lys Ala Gly Arg Leu Val Gln Ala Tyr Ile His Arg Asp

355 360 365

Gln Glu Asn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr

370 375 380

Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp

385 390 395 400

Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro

405 410 415

Leu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala

420 425 430

Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Lys Asn Pro Glu Tyr

435 440 445

Ser Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg

450 455 460

Asp Ala Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser

465 470 475 480

Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu

485 490 495

Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His

500 505 510

Ser Met Leu Ala Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys

515 520 525

Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile

530 535 540

Thr Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe

545 550 555 560

Ala Gly Asn Tyr Lys Pro Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn

565 570 575

Leu Val Leu Ile Asp Thr Thr Val Met Ala Asp Ala His Val Asp Gly

580 585 590

Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val

595 600 605

Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg

610 615 620

Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala

625 630 635 640

Asp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu

645 650 655

Lys Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln

660 665 670

Glu Asn Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn

675 680 685

Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Gly Thr Phe Lys Glu

690 695 700

Leu Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys Asp Phe

705 710 715 720

Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp

725 730 735

Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn

740 745 750

Arg Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala

755 760 765

Gln Leu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln

770 775 780

Val Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Ser Leu Val

785 790 795 800

Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala Ser Leu Leu

805 810 815

Thr Phe Gly Lys Ala Leu Lys Leu Ala Ala Thr Glu Gly Leu Gly Val

820 825 830

Leu Leu Gly Ala Pro Val Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu

835 840 845

Val Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Asp Phe

850 855 860

Asp Ser Ala Ser Phe Leu Val Ser Ile Gly Ala Ala Val Ala Gly Val

865 870 875 880

Ser Gly Val Gly Ile Val Leu Ser Gly Ala Gly Val Leu Leu Ala Gly

885 890 895

Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Glu Ile Thr

900 905 910

Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala

915 920 925

Tyr Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met

930 935 940

Pro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His

945 950 955 960

Leu Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly

965 970 975

Ser Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Val Gly Asp Met

980 985 990

Pro Lys Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln

995 1000 1005

Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala

1010 1015 1020

Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg

1025 1030 1035 1040

Tyr Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp

1045 1050 1055

Val Ile Arg Arg Leu Glu Glu Asp His Arg Phe Asp Tyr Val Phe Tyr

1060 1065 1070

Ile Phe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val

1075 1080 1085

Glu Thr Lys Val Ala Val Val Pro Asp Arg Arg Pro Leu Arg Leu Arg

1090 1095 1100

Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile

1105 1110 1115 1120

Glu Gly Thr Gly Gly Glu Tyr Val Ile Cys Leu Asn Lys Gly Ser Thr

1125 1130 1135

Val Thr Leu Phe Thr Thr Gly Ser Glu Ala Asn Lys Thr Ser Trp Ile

1140 1145 1150

Leu Asp Ala Arg Gln Ile Ile Ala Gly Asn Asp Pro Ile Ile Val Ser

1155 1160 1165

Gln Asp Ser Ile Thr Met Gly Ser Thr Thr Val His Leu Arg Asn His

1170 1175 1180

Asp Phe Asp Cys Leu Arg Ile Val Thr Ser Arg Glu Glu Leu Leu Gln

1185 1190 1195 1200

Val Asp Ser Ala Ser Arg Gln Thr Arg Val Leu Ser Glu Asp Ala Ser

1205 1210 1215

Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu

1220 1225 1230

Ala Glu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr

1235 1240 1245

Ser Val Gln Glu Gln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly

1250 1255 1260

Arg Ala His Asp Arg Met Leu Tyr Thr Asp Val Glu Asp Glu Thr Leu

1265 1270 1275 1280

Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly Glu Asp Ala Tyr Phe

1285 1290 1295

Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val Asn Ala Gly Thr His

1300 1305 1310

Gln Val Ile Thr Lys Tyr Tyr Pro Thr Val Leu Glu Asp Pro Asp Ser

1315 1320 1325

Lys Met Ala Arg Val Glu Gln Arg Gly Asn Ile Val Val Met Ala Met

1330 1335 1340

Ser Arg Leu Asn His Ser Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu

1345 1350 1355 1360

Val His Gln Glu Ser Met Val Leu Thr Ala Val Ile Gly Asn Ser Glu

1365 1370 1375

Met Leu Lys Arg Leu Arg Gln Ala Ser Thr Ile Ser Leu Thr Glu Leu

1380 1385 1390

Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr Met Asp Lys Leu Pro

1395 1400 1405

Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val Thr Ile Leu Ala

1410 1415 1420

Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp Leu Arg Thr Val Asp

1425 1430 1435 1440

Gly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr Asp Leu

1445 1450 1455

Thr Leu Thr Val Ser Ser His Lys Val Ala Gly Gln Asp Val Phe Tyr

1460 1465 1470

Phe Tyr Ser Ile Lys Glu Gln Arg Val Tyr Arg Gln Gln Gly Thr Gly

1475 1480 1485

Gln Thr Asn Asp Thr Ser Val Gly Ser Ile Pro Ile Pro Gly Leu Ser

1490 1495 1500

Asn Leu Phe Thr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly

1505 1510 1515 1520

Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr Tyr Leu Glu Gly Ile

1525 1530 1535

Asn Glu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala

1540 1545 1550

Thr Leu Glu Ala Glu Lys Ala Thr Thr Ile Ala Val Phe Gly Val Arg

1555 1560 1565

Asp Pro Gln Asn Ala Val Val Pro Ile Cys Asn Gly Glu Ala Trp Leu

1570 1575 1580

Phe Asp Leu Asp Gly Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Ser

1585 1590 1595 1600

Gln Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro

1605 1610 1615

Asn Leu Ile Pro Gln Val Gln Gln Met Leu Ser Gly Gln His Leu Arg

1620 1625 1630

Asn Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly

1635 1640 1645

Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Gly Asn

1650 1655 1660

Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Arg

1665 1670 1675 1680

Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln

1685 1690 1695

Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu

1700 1705 1710

Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu

1715 1720 1725

Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg

1730 1735 1740

Cys Arg Val Tyr Ala Val Glu Ser Lys Asn Met Ala Lys Asp Lys Gly

1745 1750 1755 1760

Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser

1765 1770 1775

Ile Val Pro Ala Glu Ile Thr Ile Pro Met Leu Ala Gly Ile Asp Phe

1780 1785 1790

Ala Leu Val Ala Ala Thr Lys Ala Thr Ile Val Ile Asp Arg Asp Asn

1795 1800 1805

Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp

1810 1815 1820

Gly Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu

1825 1830 1835 1840

Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly

1845 1850 1855

Asn Val Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Ser

1860 1865 1870

Gln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp

1875 1880 1885

Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val

1890 1895 1900

Phe Glu Leu Gly Asp Gly Cys Asp Cys Ser His Lys Thr Gln Ile Gly

1905 1910 1915 1920

Leu Cys Gly Ser Val Gly His Ser Thr His Asp Ile Ala Tyr Ile Ser

1925 1930 1935

His Ala Tyr Leu His Arg Trp Pro Ile Tyr Val Ile Ser Thr Leu Ile

1940 1945 1950

Ala

<210> 15

<211> 2028

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 15

Met Glu Glu Ala Asp Met Glu Asn Arg Gly Asn Glu Glu Arg His Ser

1 5 10 15

Gln Ser Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Val

20 25 30

Phe Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu

35 40 45

Glu Phe Leu Thr Arg Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr

50 55 60

Ala Glu Ala Val Thr Lys Ser Pro Ala Leu Pro Ala Gln Gly Trp Val

65 70 75 80

Lys Val Gly Arg Val Pro Gly Arg Gly Asp Val Leu Phe Val Gln Leu

85 90 95

Val Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr Leu Leu Gly Tyr

100 105 110

Asn Gly Glu Asn Asp Phe Pro Lys Pro Ala Tyr Val Asp Ile Pro Lys

115 120 125

Arg Gly Ala Lys Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly

130 135 140

Cys Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe

145 150 155 160

His Asp Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met

165 170 175

Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly

180 185 190

Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val

195 200 205

Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys

210 215 220

Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Asp

225 230 235 240

Pro Arg Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg

245 250 255

Arg Ala Leu Gln Asp Glu Leu Lys Lys Phe Ala Asp Met Phe Gly Val

260 265 270

Asp Pro Glu Ile Val Ser Asn Ala Val Asp Gly Lys Tyr Glu Gly Gly

275 280 285

Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg

290 295 300

Ala Arg Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu Leu Glu Thr

305 310 315 320

Lys Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr

325 330 335

Glu Phe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr

340 345 350

Asp Asn Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp

355 360 365

Leu Glu Ile Lys Lys Ala Arg Gly Thr Asn Ala Val Val Asn Ser Gly

370 375 380

Ser Ala Pro Lys Gln Pro Ser Leu Thr Val Gly Glu Arg Tyr Ala Glu

385 390 395 400

Met Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu Gln

405 410 415

Gly Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu Asp

420 425 430

Met Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr

435 440 445

Asp Glu Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp

450 455 460

Gln Glu Asn His Leu Leu Arg Gln Lys Thr Gln Glu Ile Glu Asn Thr

465 470 475 480

Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp

485 490 495

Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro

500 505 510

Leu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala

515 520 525

Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr

530 535 540

Ser Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg

545 550 555 560

Asp Ala Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser

565 570 575

Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu

580 585 590

Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His

595 600 605

Ser Met Leu Val Ala Val Ile Ala Asp Glu Gln Ser Leu Ala Pro Lys

610 615 620

Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile

625 630 635 640

Thr Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe

645 650 655

Ala Gly Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn

660 665 670

Leu Val Leu Ile Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly

675 680 685

Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val

690 695 700

Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg

705 710 715 720

Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala

725 730 735

Asp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Arg Leu

740 745 750

Lys Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln

755 760 765

Glu Asn Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn

770 775 780

Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu

785 790 795 800

Phe Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys Asp Phe

805 810 815

Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp

820 825 830

Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn

835 840 845

Arg Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala

850 855 860

Gln Leu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln

865 870 875 880

Val Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Ser Leu Val

885 890 895

Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala Ser Leu Leu

900 905 910

Thr Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val

915 920 925

Leu Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu

930 935 940

Val Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe

945 950 955 960

Asp Ser Ala Ser Phe Leu Val Ser Ile Gly Ala Ala Val Ala Gly Val

965 970 975

Ser Gly Val Gly Ile Val Leu Ser Gly Ala Gly Val Leu Leu Ala Gly

980 985 990

Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Gln Ile Thr

995 1000 1005

Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala

1010 1015 1020

Tyr Ser Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met

1025 1030 1035 1040

Pro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His

1045 1050 1055

Leu Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly

1060 1065 1070

Ser Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met

1075 1080 1085

Pro Gln Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln

1090 1095 1100

Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala

1105 1110 1115 1120

Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg

1125 1130 1135

Tyr Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp

1140 1145 1150

Val Ile Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr

1155 1160 1165

Ile Phe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val

1170 1175 1180

Glu Thr Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly

1185 1190 1195 1200

Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile

1205 1210 1215

Glu Gly Thr Gly Gly Gln Tyr Val Val Cys Leu Asn His Gly Ser Thr

1220 1225 1230

Val Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile

1235 1240 1245

Leu Asp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser

1250 1255 1260

Gln Asp Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln

1265 1270 1275 1280

Asp Phe Asp Cys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln

1285 1290 1295

Val Asp Leu Ala Ser Arg Gln Ala Arg Val Leu Ser Glu Asp Ala Ser

1300 1305 1310

Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu

1315 1320 1325

Ala Glu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr

1330 1335 1340

Ser Val Gln Glu Gln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly

1345 1350 1355 1360

Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr Thr Asp Val

1365 1370 1375

Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly

1380 1385 1390

Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val

1395 1400 1405

Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Val Leu

1410 1415 1420

Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn Ile

1425 1430 1435 1440

Val Val Met Ala Met Ser Arg Leu Asn Gln Pro Asp Lys Gly Val Asp

1445 1450 1455

Gly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu Thr Ala Val

1460 1465 1470

Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr Ile

1475 1480 1485

Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr

1490 1495 1500

Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe

1505 1510 1515 1520

Val Thr Ile Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp

1525 1530 1535

Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro

1540 1545 1550

Ile Pro Thr Asp Leu Ala Leu Thr Val Ser Ser His Lys Val Ala Gly

1555 1560 1565

Gln Asp Val Phe Tyr Phe Cys Ser Ile Lys Glu Gln Thr Val Tyr Arg

1570 1575 1580

Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Val Gly Ser Ile Pro

1585 1590 1595 1600

Ile Gln Gly Leu Ser Asn Leu Phe Thr Val Tyr Gly Glu Val Phe Ala

1605 1610 1615

Thr Thr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr

1620 1625 1630

Tyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His Pro Lys Trp

1635 1640 1645

Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Thr Ala Thr Thr Ile Ala

1650 1655 1660

Val Phe Gly Val Arg Asp Pro Gln Asn Ala Val Val Pro Ile Trp Tyr

1665 1670 1675 1680

His Ser Gly Lys Ile Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys Gln

1685 1690 1695

Leu Gln Phe Leu Gly Phe His Ser Asn Asp Gln Ala Trp Leu Phe Asp

1700 1705 1710

Leu Asp Gly Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Arg Gln Thr

1715 1720 1725

Val Asp Thr Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro Asp Leu

1730 1735 1740

Ile Pro Gln Val Gln Gln Met Leu Ser Gly Gln His Leu Arg Asn Pro

1745 1750 1755 1760

Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val

1765 1770 1775

Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Val Asn Lys Ala

1780 1785 1790

Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly Asn Leu

1795 1800 1805

Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln Gly Asp

1810 1815 1820

Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile Val

1825 1830 1835 1840

Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly Met

1845 1850 1855

Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Asn Thr Ser Pro Arg Arg

1860 1865 1870

Arg Ser Ile Val Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Ile

1875 1880 1885

Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile Asp Arg

1890 1895 1900

Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp

1905 1910 1915 1920

Gln Asp Gly Gly Ala Lys Val Val Leu Gly Ser Glu Ile Pro Met Lys

1925 1930 1935

Val Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn

1940 1945 1950

Asn Gly Asn Val Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala

1955 1960 1965

Cys Cys Gln Ile Asp Ile Lys Val Gly Asp Ala Glu Thr Met Leu Ala

1970 1975 1980

Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser

1985 1990 1995 2000

Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser

2005 2010 2015

Asp Tyr Glu Asp Leu Trp Ala Ile Leu His Met Thr

2020 2025

<210> 16

<211> 51

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 16

<210> 17

<211> 93

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 17

gggcggtacc ccgggttcga acactagtta attaagacgc gt 42

<210> 18

<211> 16379

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 18

gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 60

acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 120

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 180

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag 240

cttgccaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc 300

tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc 360

ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc 420

acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac 480

agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca 540

accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga cactctcgtc 600

tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa 660

caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc 720

aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag 780

gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg 840

agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat 900

atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga ccttcctcta 960

tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa 1020

atctatctct ctcgagtcta ccatggctca gatcaggtcg atggctcagg gcattcagac 1080

gctgtcgctc aactcctcta atctctccaa gacgcagaag gggccgctcg tgtcgaactc 1140

tctcttcttc ggcagcaaga agctgacaca gatctctgcc aagtcactgg gggttttcaa 1200

gaaggactcg gtgctccggg tggtccgcaa gtccagcttc cgcatctcag cttccgtcgc 1260

tacagctgag gctgaggccg tgatcgctga ggtctccact cagctcagcg aggttgtggg 1320

cgtgatcgag aggcacctgg agccaaccct cctggctgtc catctctacg ggtcagcggt 1380

tgatggtggc ctgaagcccc actccgacat cgatctcctg gttacagtga ctgtccggct 1440

ggacgagacc acgaggaggg ctctcattaa cgatctcctg gagaccagcg cttcgccagg 1500

cgagtccgag atcctcaggg cggttgaggt gacgattgtc gttcatgacg atatcattcc 1560

atggaggtac ccagctaaga gggagctcca gttcggcgag tggcagcgca atgacatcct 1620

ggccgggatt ttcgagccag cgacaatcga cattgatctg gctatcctcc tgactaaggc 1680

tagggagcac tccgtcgctc tggttggccc tgctgctgag gagctcttcg acccagtccc 1740

tgagcaggat ctcttcgagg ccctgaacga gaccctcacg ctgtggaatt ctccgcccga 1800

ctgggctggc gatgagagga atgtggtcct caccctgtcg cgcatctggt actctgctgt 1860

cacggggaag attgctccaa aggacgtggc tgctgattgg gcgatggaga ggctgccagc 1920

tcagtaccag cctgtgatcc tcgaggctag gcaggcttac ctgggccagg aggaggacag 1980

gctcgcgtcc cgggctgatc agctggagga gttcgttcat tacgtcaagg gcgagattac 2040

taaggtcgtt ggcaagtgac tcgagtttct ccataataat gtgtgagtag ttcccagata 2100

agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt 2160

atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat 2220

ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg 2280

tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc 2340

caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag 2400

gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc 2460

tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat 2520

gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca 2580

ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt 2640

tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt 2700

cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa 2760

taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc 2820

tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat 2880

gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa 2940

cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac 3000

tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc 3060

tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc 3120

atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc 3180

cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac 3240

tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa 3300

aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg 3360

aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg 3420

tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc 3480

gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt 3540

ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt 3600

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 3660

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 3720

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 3780

ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 3840

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3900

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3960

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4020

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4080

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4140

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4200

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4260

cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4320

tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 4380

accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc 4440

cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 4500

gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg 4560

cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 4620

ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 4680

catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac 4740

ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg 4800

ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg 4860

gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg 4920

cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc 4980

tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca 5040

atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca 5100

ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc 5160

gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg 5220

atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg 5280

atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg 5340

tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg 5400

tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg 5460

tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg 5520

tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc 5580

tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc 5640

gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct 5700

cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct 5760

cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc 5820

aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg 5880

tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa 5940

acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg 6000

aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc 6060

ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg 6120

cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc 6180

aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc 6240

cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct 6300

tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg 6360

ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc 6420

atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct 6480

tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt 6540

tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc 6600

gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc 6660

attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc 6720

gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct 6780

cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc 6840

aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag 6900

ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca 6960

tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga 7020

cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat 7080

ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc 7140

ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac 7200

atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga 7260

cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta 7320

tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca 7380

catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc 7440

gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc 7500

gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg 7560

aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat 7620

tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc 7680

gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc 7740

gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct 7800

cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc 7860

gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact 7920

cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc 7980

acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca 8040

gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag 8100

ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct 8160

tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt 8220

cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca 8280

tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga 8340

caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg 8400

acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc 8460

ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata 8520

caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac 8580

gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg 8640

aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct 8700

gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 8760

agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 8820

ttgtaaaacg acggccagtg ccaagctaag cttgcatgcc tgcaggtccc cagattagcc 8880

ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcaggtc 8940

tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg 9000

ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat 9060

ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa 9120

ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg 9180

agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca 9240

tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg 9300

acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg 9360

agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct 9420

gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg 9480

ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc 9540

cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg 9600

attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag 9660

acccttcctc tatataagga agttcatttc atttggagag aacacggggg actctagagg 9720

atcgcgaggc gcgccgtcga catggactac aaggacgacg atgataaggg catggactac 9780

aaggacgacg atgataaggg catggactac aaggacgacg atgataaggg cggtaccccg 9840

ggttcgaaat ggttatggta attgactatc caagtctcag agcttcgtta tatgccacta 9900

agagtgcggt cccatgtcgc ccgcttccgg gcaatccaga gcgggtgtcg cgccgggtat 9960

cacgcagagg cgcgggagag atcgggtccc atgcagattt ctggatctcc cgcgccaagg 10020

catccgccat ccggattttc ccgcgtcaag agggtccgct ccgtgaatcc cggagaagat 10080

tctatcttct ccgcgaagcg gtgttcaccg gccgcaggac cgcctgggcc gctaatccag 10140

cggcccaacg ggcagcatgg gaccgcaaga gatccccttt cttcggaagg tcttccgatg 10200

atattcattc attcagcaag aatccagaag aattcttgac tcaaaacact ctcagctgca 10260

gtttattaac tcgcaccgct cagaccgtca caaaaagccc agtattgccc gcccaaggtt 10320

gggtcaaagt gggtcgtgtg ccaggaagag gagacgctct ctttgttgag ctggttgccg 10380

agaaatcaga tgagacagtg aaggcgtact tacttggcta caatggtaag aacgactccc 10440

ccgagccggc atacgttgat attccaaaga ggggcgccga gaacggaaca tttttgttta 10500

ccgtggatct ctcgggctgc tccgttgttg tgacacagtt ggacgagcag acataccgtg 10560

ttttccacga caaacgggtg aacagctcca ttctgtacga caacgtcgta atgtatgtgg 10620

atttcaatca ctaccgctat cccagagccc acggacacgg gagagatacg ggacaagcgg 10680

tcgtctgtat gcaattcgcc gacggagtgt ggaaattgtt tctccagcgg cacgagacgg 10740

cgaattctct cccaaagata tggtcccgtg gcgatggcaa accggagatt ttcagcgacg 10800

ccgaaccccg tccacatatg gaagatggca taaaggaatt tctcaatcgg cgtcgagctt 10860

tgcaagacga attgaagcac ttcgccaaca cgcttgaagt ggacccggac atagtgtcca 10920

acgctgtcga cggcgaatac gaaggcggcc agttttccag gaacgacgat gcaattcgtg 10980

gatggaacga attacgtgca agcatgagac agaaaatggc tgaaaggaag aaactgttgc 11040

aaacaaagaa aggtatgcta gactcattca acaacccgga tcaccccctc tataaatttc 11100

gcaaacaagt gctcgacgag ctcaaggaaa tgagtgcata cgacaacacc ttggaggcat 11160

ccgagattgc ggaccgtact tggctgtggt tggagatcaa aaaagccaaa ggtacgaatg 11220

cagtggtaaa cagtggagca gccccgaagc agccgagtct taccgttggt gaacgctacg 11280

cagagatgga ggcggccctt tggatgcgta gcgacacagc ggcgtttgag caagggtatg 11340

aatcttatca aaccgttcgc gtgccgggtt acaatgaaga tatgacatcc attgcaatga 11400

agaagttgtt cgttgagagc agtctgactg acgagcagct gggcgccttg tgcaagcgta 11460

tatccattgc tagcgatcaa gagaaccatc ttctgacaca gaagacgcag gagattgaga 11520

gcactttcaa acaattttca gatggcagct ttttggagtc ggcgcctcag gatttcttcc 11580

tgagacgagt cggtgataaa tctggaggac gctgctatcc tcttgtaagg gtaatggccg 11640

tcgcactgtc tgaaggagaa gacgttgccc agaattttat ggagaacctg aggacattgt 11700

ctaggaatcc agagtattca ggcgaatcgg caagttcaca agccacaaat gcagggttgt 11760

tcagagatgc cctggccggc ctgcacgcga atgtggaagc cattgccgca tctgagaaac 11820

tacctgctgt gtttggactg cccgatgttg ctacaatgtt gagccaagcg agcgaaagca 11880

aggtttacgc tttgaatacc gaaacacaca gcatgctggt tgcggtgatt gtagacaaac 11940

agagtctggc gcgcaagtat tacttctatg atcccaactt tctcatggcc ggtttctccg 12000

acataactcg tttctcggaa gccctcaagt atcactttgt gaatctccag ttcgccggaa 12060

actacaaagc gtttggcaca gcggaggctc cccagtttaa cctggtcctt attgatacga 12120

ccgccatgga ggatgcgcat gttgacggag ggatcaccgt gaaacaattg agcgagggcg 12180

ttgatcttac taatgtcttt gacagcacca aaaaggctcg tgtggtaact gaggcagcag 12240

ctcgcttggc tgaagaccaa caactgcgca ctggcctcac cataatagag gctgaccagt 12300

gggcaaacag gtggcaagat gccgtcatac gattgaaact caaggaagga ttaagtggag 12360

agtggatgcc attgctggag aaaatgcaag aaaacgcaga tggttcttat cagattgatt 12420

tcatcaacac caagaatact ggggaaatcc gtggggtttc cactagagat gcgacgttca 12480

aagaattcaa atcctacctg gataaaaatt tagacgtatt gcataaggat ttcatctttc 12540

aggatggagt tttcaaacca ctggagggag agcccattga cgggttgaat gcaatgttcg 12600

cggtccaaat gattatagat tggttcaacc gggggactaa tgctgaggaa gatgcggcct 12660

caggatccac caatgcccaa ctttccctag cgattaaagt gcacagctac attaacatgg 12720

cccaagtagc gcatactgca gttacagatg ttgccaaggt atacaacctt gtaaagtcag 12780

cactgtatgg cgagcaggta gctggggaga catcgctgtt cacatttggg aaggcgttga 12840

aatttgcagc aacagaaggg ttgggagtct tactaggggc gtcctttgtg atcttggatg 12900

cctaccagct gtccctcgtg gaaaacgatg tgcagaaggc cattatcggc acacaactag 12960

cctttgattc cgcaagcttt ctagcgggta ttggtgcaat tgttgctgga gcatcgggcg 13020

tgggcatgat tttgggcgga gctggcgtgg tattgggagg cctgggagtt ggctttggag 13080

ccttagctca ggcattcggg gaaatcacag cagacgccca actggtgggc aagtacttcg 13140

cactcttgga cgaggcatac cgcggtggcg gatacagata cgaatctaaa gacaaggcat 13200

tgatgcctct tgcaggagca gtcatcgaca aaatacgact ggccgacaaa catctccaag 13260

ttcatttcga cagccaattc atatatcgaa cccatcatgg cagcacagga tccggcaaaa 13320

ttaattactt cttctgggct ggcgacatgc cgcagatggt ccacgatcgc tcccaagcta 13380

tcaatgttcg agagcaaatt gggtataaga acgataatgc tactttggag ctaccggagg 13440

atgcctctga tctgatcctg cctgccacgc ccaagtcatt tatcacttac cgatacgaga 13500

ttctaccatt cgctacttcc cgccacgata cgggtttcga tgtcattaga cgcctggaag 13560

aagactacag attcgattac gatttctaca tcttcccatc tgaatacaca atcagaacca 13620

tcaaacacga gtacgtagaa acaaaggtgg cagtagtcct ggataggcgc tctttgcgac 13680

tgggagcacc tgagcttctc aaggagcaac atggctattt gatctatgag atcgagggga 13740

ccggaggaca atacgttata tgtcttaacc agggatccac tgtcacactg aagaccaccg 13800

gatcagaggc aaataaaaca ttatggatac ttgatgcccg acaactcatt gcaggcaacg 13860

attcaatcac ggtctctcaa gatagcatca ccatcggcag cacaacagtc catctccgta 13920

accaagattt cgattgccta cagatcgtca tgagtcggga ggagcttctc caagtggact 13980

tggcgtctgg agaagcccgc gtgttttcag aagatgccag taaatggctc gacaccaaag 14040

aaacaatcca tgaacatctc cataagttgg ctgaagaaca caggttgcat ggccagtttg 14100

tagtggtgaa caattattat cgtggagtgg gccgtgccta ctatgatgct gctcacgatc 14160

ggatgttgta tacagatgtg gaagatgaaa ctctgactcg ggatgctgtt cttgccggcg 14220

ttacaggcga agatgcctac ttctacaatg taaagcagaa tgccctgtgg cgagtgaatg 14280

caggcacaca ccaagtaata accaaatact atccgacgtt actggaggac cctgacagca 14340

acatggcaag agttgagcag catggcaaca tcgtcgtgat ggccatgagc aggctcaacc 14400

acccggacaa gggagtaaat ggaatcatct atctcgttca tcaagaaagc atcgtgctta 14460

ctgctgtgat cggcaattca gagatgctca aacgtcttcg ccaaacgagt acaattagct 14520

taacagaatt gcgaggtgtc aatgacatgg ctctggtttc cacgatggac agattacccg 14580

gccaaaaggt tgtcgctacc aacgctgagt ttgtgacact tctggctaag gatgagcaag 14640

gagccaatat caaggagcag acggtctatt gccaacaagg tacgggccag accaacgata 14700

catctattga aagcatcccg attccagggc tctctaatct tttaaccgtg tacggtgaag 14760

tattcgctac aactaatgat ggtcgcatcc ttcgcctgct tggggacggc tcaacatgtc 14820

tcgagggcat caatgagcat tggctcatca agcatccaaa atggtggcag gacttggcta 14880

ccttggaggc agagaaagcc acgacagttg cagtattcgg tgttagagac ctacaaaatg 14940

cagtggtgcc aatttggtac cacaacggaa agatcgcggt ggcctcatca aaactgtatg 15000

gcaaacaact gcaatttttg gggtttaaca gcaacggtga agcttggttg ttcgacttgg 15060

acgacggcat tctgtatagc cagccgcttg tggatagctt tctgtatgca atctttagtt 15120

tggacgggaa gcttattgca ccaatcttta ttccacaagt ggagcaaatg ctcagcggcc 15180

agcatctcag aaaccctgtc atcgtggacg gccgtttgca tgtgactacc atagacggtg 15240

ttgttgttgg atttgacaag aagtcgccaa ctctggttgc agtgaacaag gcctggcaga 15300

tatctcgtca gaaccgatta gaagaggccc tcggaaacct gctgtccaat tggtctgatc 15360

acaatgatgc gattgtgttg cagggtgatc cagaatcagc gccggcatgg tattatgttc 15420

catcaagaga aatagtgacc gccaatggtc taacatgggc ggatcatcct ctatggctag 15480

gaatggctgt cggtgctgca acgccataca tctacagcac atcccgacgc cgtgtgtatg 15540

ttgtggagag caaaaacatg gccaaagaca aaggagaatt cgcagcagca cattgctttg 15600

caaaatcact tgtgctacag agcatcgttc ccgaggaaat aacgattccg acgctggctg 15660

gcatcgattt cgcactcgtt gcagcgacaa atgcaaccat tgtaatagac cgagacaatt 15720

ggaagcatta caaggccatt gtgctcgaca acaaggtgga tcaggacggt ggcgctaagg 15780

tggtagtcgg cagtgagatt cccatgaaag ttctggtgcg caaatttcag ggaaatctgg 15840

agttcttcga tccaaacaat ggtaatgttg ttatactcca gaatgtgttg aagggagacg 15900

gagcttgttg ccagatagag atcaaagtag gcgatgctga gactatgctt gtatccgatt 15960

tctcggagat attctcaaag acgcatcctc tggtggcaag ccaagtattt gagcttgggg 16020

atgtgattgc agtcacaaaa cacaaatcgg actatgcgga tctgtgggcc attctacgca 16080

tgacatagac gcgtcttaat taactagtga gctcgaattt ccccgatcgt tcaaacattt 16140

ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat 16200

ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga 16260

gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa 16320

tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgg 16379

<210> 19

<211> 50

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 19