首页> 中国专利> 一种应用于酸性小分子定性定量分析检测和高通量筛选增强电离缓冲液试剂盒及其制备

一种应用于酸性小分子定性定量分析检测和高通量筛选增强电离缓冲液试剂盒及其制备

页面导航

摘要
著录项
法律信息
相似文献

摘要

一种应用于酸性小分子定性定量分析检测和高通量筛选常规增强电离缓冲液试剂盒及其制备，若待测目标小分子是酸性结构，pH＜4，加入同等体积的增强电离缓冲液QLB1；充分混合后，取样品点在靶板上即可送入表面激光解析质谱进行分析；QLB1物质组成及体积比为：50-70％甲醇，30-50％离子水，1-7％三氟乙酸，此试剂盒制备方法简单、成本较低。

著录项

公开/公告号CN104266886A

专利类型发明专利
公开/公告日2015-01-07

原文格式PDF
申请/专利权人武汉品生科技有限公司;
展开▼

申请/专利号CN201410453832.9
发明设计人成晓亮;郑可嘉;
展开▼

申请日2014-09-09
分类号G01N1/28(20060101);G01N27/64(20060101);
代理机构32207 南京知识律师事务所;
代理人胡玲
地址 430040 湖北省武汉市东西湖区七雄路海峡创业城二期1栋303
入库时间 2023-12-17 03:45:10

法律信息

法律状态公告日

法律状态信息

法律状态
2017-09-19

实质审查的生效 IPC(主分类):G01N1/28 申请日:20140909

实质审查的生效
2015-01-07

公开

公开

相似文献

专利
中文文献
外文文献

1. 一种应用于酸性小分子定性定量分析检测和高通量筛选增强电离缓冲液试剂盒及其制备 [P] . 中国专利： CN104266886A . 2015-01-07
2. 一种应用于污染物小分子定性定量分析检测和高通量筛选增强试剂盒及其制备 [P] . 中国专利： CN104215685B . 2017.04.19
3. isolated polynucleotide, expression cassette, host cell, isolated polypeptide, non-human transgenic animal that has been altered to express an alkali resistant mutant polypeptide, an antibody that specifically binds to an alkali resistant mutant polypeptide, a mutation detection kit alk inhibitor resistance in a biological sample, and methods for evaluating a biological sample for an alk inhibitor resistance mutation to diagnose cancer that is resistant to or likely to develop resistance to at least one alkaline inhibitor. small alk molecule kinase, to evaluate a biological sample for an alk inhibitor resistance mutation, to diagnose cancer that is resistant to or likely to develop resistance to pf-02341066 in a patient, to specifically reduce expression of an alk-resistant mutant to treat cancer associated with activating aberrant loop capacity that is resistant to at least one small molecule alk kinase inhibitor treating an aberrant cancer that is resistant to pf-02341066, identifyin [P] . BR112012026239A2 . 2018-06-26

机译：分离的多核苷酸，表达盒，宿主细胞，分离的多肽，已被改变以表达耐碱突变体多肽的非人类转基因动物，与耐碱突变体多肽特异性结合的抗体，突变检测试剂盒生物样品，以及用于评估生物样品中是否存在对烷基抑制剂的抗性突变以诊断对至少一种碱性抑制剂有抗药性或可能发展出抗药性的癌症的方法。小分子激酶，用于评估生物学样品中是否具有抑制碱性磷酸酶抑制剂的突变，以诊断对患者具有抗药性或可能对pf-02341066产生抗药性的癌症，从而特异性降低抗癌突变体的表达以治疗癌症与激活抗至少一种小分子烷基激酶抑制剂的异常环路能力有关，该抑制剂治疗对pf-02341066有抵抗力的异常癌症
4. Polinucleotu00eddeo insulated tape of expression, host cell, isolated transgenic non-human animal polypeptide, which has been modified to express a polypeptide mutant with resistance to ALK, antibody that binds specifically to a polypeptide mutant with resistance to ALK, kit p For detection of a mutation with resistance to ALK inhibitor in a biological sample, and,Methods for evaluating a biological sample as a mutation with resistance to ALK inhibitor, for diagnosing a cancer that is resistant to or is likely to develop resistance to at least one inhibitor of kinase small molecule ALK, to evaluate a biological sample that Anto a mutation with resistance to ALK inhibitor.For diagnosing a cancer that is resistant to or is likely to develop resistance to at least one inhibitor of kinase small molecule ALK, to assess the biological sample as a mutation with resistance to ALK inhibitor, a cancer that is resistant to dianosticar Or is likely to develop resistance to PF - 02341066 in a pati [P] . BR112012026239A2 . 2016-11-29

机译：修饰的多聚核苷酸绝缘带，宿主细胞，分离的转基因非人类动物多肽，经过修饰以表达对ALK具有抗性的多肽突变体，与对ALK具有抗性的多肽突变体特异性结合的抗体，试剂盒p用于检测生物样品中对ALK抑制剂具有抗性的突变，以及用于评估生物样品为对ALK抑制剂具有抗性的突变的方法，用于诊断对至少一种对ALK抑制剂具有抗性或可能发展出抗性的癌症激酶小分子ALK，以评估Anto对ALK抑制剂具有抗性的突变的生物样品。用于诊断对至少一种激酶小分子ALK抑制剂具有抗药性或可能产生抗性的癌症，以评估该生物样品作为对ALK抑制剂有抗性的突变，即对狄诺司他抗性或可能对PF产生抗药性的癌症-02341066
5. Methods for determining the relative proportions of components and for providing a liquid mixture with pre-set pH and ionic strength, high throughput screening procedure, liquid chromatography system and process, and buffer preparation device. [P] . BRPI0911221B1 . 2019-05-14

机译：用于确定组分的相对比例并提供具有预设的pH和离子强度的液体混合物的方法，高通量筛选程序，液相色谱系统和方法以及缓冲液制备装置。