The Influence of Cultural Conditions and Concentration Procedures on the Antigenicity of Mycoplasma Pneumoniae Vaccines, (Including Studies on Respiratory Syncytial Virus).

摘要

The third experimental mycoplasma pneumoniae vaccine produced at Huntington Research Center produced 100% serologic conversion in human volunteers who were seronegative prior to injection. This vaccine differed from the previous two lots produced at Huntington Research Center (HRC) in that (1) it was prepared from a low passage mycoplasma seed (2) the seed was from a new isolate which had never been grown on medium containing horse serum and (3) considerably more antigenic mass was present in the vaccine. The adjuvanting effects of Poly 1:C in mycoplasma and respiratory syncytial virus vaccines has been investigated. Addition of this material to both mycoplasmas and respiratory syncytial virus vaccines produces a marked adjuvanting effect. No adverse sensitization effect either on parenterally or intranasally administered vaccines has been demonstrated. Respiratory syncytial virus has been separated from media components and cells by various physical methods. The viral material has been further separated into two component antigens and antisera in rabbits have been prepared to each of the antigens. In addition,hamsters have been injected with these purified materials and studies are at present in progress to determine whether there is an enhancement of the pathogenetic effect of infection with RS in animals previously injected with either of the separate antigens.