Osteoblast Differentiation and Extracellular Matrix Mineralization in Response to Pore Size and Shape: Static Culture

摘要

Poly(ester urethane)urea (PEUUR) foams have been designed to cure in situ upon injection to form porous scaffolds. In this study, the effects of water concentration and polyester triol composition on the physicochemical, mechanical, and biological properties of PEUUR foams were investigated. A liquid resin (lysine diisocyanate: LDI) and hardener (poly(-caprolactone-co- glycolide-co-DL-lactide) triol, tertiary amine catalyst, anionic stabilizer, and fatty acid-derived pore opener) were mixed, and the resulting reactive liquid mixture injected into a mold to harden. By varying the water content over the range of 0.5 to.75 parts per hundred parts polyol, materials with porosities ranging from 89.1 to 95.8 vol-% were prepared. Cells permeated the PEUUR foams after 21 days post-seeding, implying that the pores are open and interconnected. In vitro the materials yielded non-cytotoxic decomposition products, and differences in the half-life of the polyester triol component translated to differences in the PEUUR foam degradation rates. We anticipate that the injectable PEUUR foams will present compelling opportunities for bone tissue engineered and delivery systems due to their favorable biological and physical properties.