A Method of Preparing the 'in situ' Frog Spinal Cord for Intracellular Recording.

摘要

A method is described for maintaining the frog spinal cord in situ and for recording intracellularly from lumbar motoneurons in this preparation. The spinal cord in this preparation appeared to be healthier than in the excised, in vitro, preparation. Furthermore, the preparation is especially useful for keeping peripheral nerves, as well as the brain, in connection with the spinal cord. The development of this technique will greatly assist the study of this nervous system, which is of importance because it is of a complexity intermediate between that of higher vertebrates and invertebrates. This model will be used for studies in toxicology and on mechanisms of movement.