Assessment of the Technical Requirements of a Closed Blood Handling System Usinga Tri-Enzymes for a HIV Assay. Phase 1

摘要

This funded research effort assessed the technical requirements to develop aclosed blood handling device and testing vehicle for Human Immunodeficiency Virus (HIV) utilizing ELISA protocol as well as the encapsulation of the multi-enzymes within the closed system. It was a design/modeling program to modify an existing single cavity cuvette developed for Non A, Non B Hepatitis to the multi/ process system required by the ELISA protocol. The Auto-cuvette prototype designed within this Research Project is a seven stage automated blood testing vehicle using Wafer Technology to encapsulate the multi-enzymes required by the ELISA protocal. The design allows complete safety from HIV contamination; (1) The coated cuvet cell is received from the supplier with a sealing foil in place for operator protection. This foil will be pierced at the first stage, (2) All offal from both wash stations is carried into a disposable reservoir, and (3) The coated cuvet cell used for absorbance measurement is automatically sealed before removal from the Auto-cuvette.... SBIR; RA I; Mathematical Modeling; Blood Testing; HIV; Elisa.