Heat Shock Protein Induction in Human Cells by CO2 Laser Irradiation.

摘要

This study investigated the effect of millisecond exposures from a carbon dioxide (CO2) laser on the production of heat shock proteins in human fibroblast cell cultures. Heat shock or stress proteins (hsp) are induced to high levels by heat shock, transition series and heavy metals, amino acid analogs, certain chemicals, and ultraviolet radiation. These proteins are highly conserved throughout nature and their regulation and cellular functions are just beginning to be understood. The production of hsps was monitored by 35S-methionine incorporation into newly synthesized heat shock protein and by immunocytochemistry. It was shown that CO2 laser irradiation could induce the production of hsps. The time course of hsp synthesis and localization within cells was also determined. The hsp synthesis produced by the CO2 laser was compared to the effects of sodium arsenite and heat. The results of this study indicate that hsp 70 is the major heat shock protein induced by CO2 laser radiation and that this response is most likely due to the thermal effects of the laser.... Heavy metals, Immunolocalization, Sodium arsenite, HSP 70.