THE USE OF PCR AMPLIFICATION IN DETERMINING THE TOXIGENIC POTENTIAL OF FUSARIUM SAMBUCINUM AND F. SOLANI ISOLATED FROM POTATO TUBERS WITH SYMPTOMS OF DRY ROT

摘要

Fusarium sambucinum and F. solani are the main causes of the dry rot of potato tubers. PGR amplification was used to (i) confirm the taxonomy of 28 isolates of F. sambucinum and 24 isolates of F. solani which had been isolated from various potato crops in Poland and identified on the basis of their morphology in vitro, and (ii) study the potential of F. sambucinum and F. solani to produce trichothecene myco-toxins. All F. sambucinum isolates, but none of F. solani isolates, showed the presence of theTW5 gene which encodes for production of trichothecenes. Fusarium sambucinum isolates did not have the potential to produce the B-type trichothecenes, the group that includes deoxynivalenol (DON) and nivalenol (NIV). Chemical analyses of potato tubers inoculated with F. sambucinum showed the presence of the A-type trichothecenes: monoacetoxyscirpenol (MAS) and diacetoxy-scirpenol (DAS).