Efficient plant regeneration from leaf and petiole explants of strawberry (Fragaria x ananassa Duch.).

摘要

An investigation was conducted to standardize a protocol for plant regeneration using leaf and petiole explants in strawberry (Fragaria x ananassa Duch. cv. Chandler). Out of a total of 62 different combinations and concentrations of auxins, cytokinins and thidiazuron tried for leaf explants, 11 media responded to shoot regeneration. The maximum direct shoot regeneration (48.20%) was obtained on modified Murashige and Skoog's medium containing B₅ vitamins, 2% glucose, 1.0 mg/l thidiazuron, 1.0 mg/l N6-benzyladenine and 0.5 mg/l indole-3-butyric acid. Highest indirect shoot regeneration (36.40%) from leaf explants was obtained on MS medium supplemented with 2.0 mg/l N6-benzyladenine and 1.0 mg/l indole-3-butyric acid. For petiole explants, out of 18 different combinations and concentrations of growth regulators tried, only one (modified MS medium containing B₅ vitamins, 2% glucose, 1.5 mg/l N6-benzyladenine and 0.5 mg/l indole-3-butyric acid) lead to direct shoot regeneration (12%). Root regeneration in in vitro developed shoots was obtained on half-strength MS medium supplemented with 1.0 mg/l indole-3-butyric acid. The regenerated complete plantlets were transferred to pots and acclimatized. Thus, a reproducible and efficient plant regeneration protocol has been standardized.