Effects of the noradrenergic neurotoxin DSP-4 on the expression of α 1-adrenoceptor subtypes after antidepressant treatment

摘要

We have previously reported that chronic imipramine and electroconvulsive treatments increase the α 1A-adrenoceptor (but not the α 1B subtype)mRNAlevel and the receptor density in the rat cerebral cortex. Furthermore, we have also shown that chronic treatment with citalopram does not affect the expression of either the α 1A- or the α 1B-adrenoceptor, indicating that the previously observed up-regulation of α 1A-adrenoceptor may depend on the noradrenergic component of the pharmacological mechanism of action of these antidepressants. Here, we report that previous noradrenergic depletion with DSP-4 (50 mg/kg) (a neurotoxin selective for the noradrenergic nerve terminals) significantly attenuated the increase of α 1A-adrenoceptor mRNAinduced by a 14-day treatment with imipramine (IMI, 20 mg/kg, ip) and abolished the effect of electroconvulsive shock (ECS, 150 mA, 0.5 s) in the prefrontal cortex of the rat brain. The changes in the receptor protein expression (as reflected by its density) that were induced by IMI and ECS treatments were differently modulated by DSP-4 lesioning, and only the ECS-induced increase in α 1A-adrenoceptor level was abolished. This study provides further evidence corroborating our initial hypothesis that the noradrenergic component of the action of antidepressant agents plays an essential role in the modulation of α 1A-adrenoceptor in the rat cerebral cortex.