Multiplexed SSR markers for identification and purity assessment of Canadian flax varieties

摘要

A multiplexed set of SSR markers was assembled which, in combination with DNA extraction from individual seeds, provides a practical means to identify Canadian flax varieties and may be useful for assessment of seed purity. Seven SSR markers were chosen based upon their joint ability to discriminate among varieties and, following selection of alternative primers for three of the markers, were combined into a single multiplex with no overlap of allele sizes. A survey of 120 seeds of each of 23 registered Canadian flax varieties indicated that polymorphism within varieties was common and may add a layer of complexity to variety identification and, in particular, to seed purity analysis. Examination of large numbers of individual reference seeds is recommended when constructing databases to ensure that polymorphism that may exist within varieties is sufficiently represented.