Encapsulation of cabbage seeds in alginate polymer containing the biocontrol bacterium Pseudomonas fluorescens strain LRB3W1 for the control of cabbage soilborne diseases

摘要

Cabbage seeds were encapsulated in alginate polymer containing an antagonistic bacterium, Pseudomonas fluorescens strain LRB3W1. Seed germination was not inhibited by the encapsulation. Seedlings were transplanted into soil infested with Rhizoctonia solani, a pathogen which causes cabbage damping-off. A week after treatment, damping-off in encapsulated seedlings was lower than that of untreated control. Additionally, 2 weeks after germination, seedlings were inoculated with Fusarium oxysporum f. sp.conglutinans, a pathogen of cabbage yellows. The yellows disease was less severe with bacterial encapsulation treatment compared with the untreated control. The bacterium colonized in the cabbage rhizosphere after germination of encapsulated seeds. The bacterium survived in the alginate polymer for a prolonged period at 4 deg C. The encapsulation of cabbage seed with the biocontrol bacterium was effective for protection against cabbage soilborne diseases.