Radiation sensitivity of bacteria and virus in porcine xenoskin for dressing agent

摘要

In this study, gamma irradiation sensitivities of bacteria and viruses in porcine skin were evaluated to establish the optimum sterilization condition for the dressing material and a xenoskin graft. Escherichia coli and Bacillus subtilis were used as model pathogens and inoculated at 10~6-10~7 log CFU/g. As model viruses, porcine parvovirus (PPV), bovine viral diarrhea virus (BVDV), and poliovirus were used and inoculated at 10~5-10~6 TCID_(50)/g into porcine skin. The D_(10) value off. coli was found to be 0.25 ± 0.1 kGy. B. subtilis endospores produced under stressful environmental conditions showed lower radiation sensitivity as D_(10) was 3.88 ± 0.3 kGy in porcine skin. The D_(10) values of PPV, BVDV, and poliovirus were found to be 1.73 ±0.2, 3.81 ±0.2, and 6.88 ±0.3 kGy, respectively. These results can offer the basic information required for inactivating pathogens by gamma irradiation and achieving dressing material and porcine skin grafts.