Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium

摘要

This paper describes a platform for real-time sensing of dissolved oxygen in a flowing microfluidic environment using an oxygen-sensitive luminescent dye (platinum octaethylporphyrin ketone) integrated into a micro-oxygenator device.Using a phase-based detection method,the luminescent decay lifetime of the dye was consistent with the linear Stern-Volmer relationship using both gaseous and aqueous samples.Maximum sensor resolution varied between 120-780 ppb across a range of dissolved oxygen (DO) concentrations ranging from 0-42.5 ppm.The sensor was subsequently used to determine the convective mass-transfer characteristics of a multilayer polydimethylsiloxane (PDMS) microfluidic oxygenator.The membrane-based oxygenator showed excellent agreement with an analytical convection model,and the integrated oxygen sensor was accurate across a wide range of tested flow rates (0.05-5 mL min~(-1)).The device is unique for its ease of fabrication and highly flexible configuration,as well as the novel incorporation of oxygen delivery and detection in a single micro-device.Potential applications include tissue engineering,cell culturing,and miniaturized bio-assays that require the delivery and/or detection of precise quantities of oxygen within a microfluidic construct.