Chitosan-coated PLGA nanoparticles for DNA/RNA delivery:effect of the formulation parameters on complexation and transfection of antisense oligonucleotides

摘要

Cationically modified poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles have recently been introduced as novel carriers for DNA/RNA delivery.The colloidal characteristics of the nanoparticles-particle size and surface charge-are considered the most significant determinants in the cellular uptake and trafficking of the nanoparticles.Therefore,our aim was to introduce chitosan-coated PLGA nanoparticles,whose size and charge are tunable to adapt for a specific task.The results showed that biodegradable nanoparticles as small as 130 nm and adjustable surface charge can be tailored controlling the process parameters.As a proof of concept,the overall potential of these particulate carriers to bind the antisense oligonucleotides,2'-O-methyl-RNA,and improve their cellular uptake was demonstrated.The study proved the efficacy of chitosan-coated PLGA nanoparticles as a flexible and efficient delivery system for antisense oligonucleotides to lung cancer cells.