The study aim was to evaluate the effect of an acute fluid deprivation (FD) on mood and physiological parameters. Twenty healthy women (aged 25 ± 3.5 years) participated in a randomized 2-period (dehydrated vs control) crossover study. In the FD period, the last water intake was between 6 PM and 7 PM, and no fluid intake was allowed up to 6 PM on the next day. The FD resulted in increased sleepiness and fatigue, decreased alertness, and increased confusion. In this rigorously controlled protocol, the early noninvasive markers of dehydration were a reduced urine volume, increased urinary gravity, darker urine color, and increased thirst. Interestingly, dehydration also induced a significant increase in saliva osmolality at the end FD period. Plasma osmolality did not differ between experimental conditions. ABBREVIATIONS: CG-control groupCI-confidence interval,IG-intervention group,SDS-SD score.
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