Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.

摘要

The sheep beta-lactoglobulin gene is expressed efficiently and at high levels in the mammary gland of transgenic mice. In contrast, when this gene is linked to a 2nd gene construct comprising a mammalian cDNA or a chloramphenicol acetyltransferase (CAT) reporter sequence it fails to be expressed in the majority of transgenic lines generated. It is suggested that mammalian cDNAs and prokaryotic reporter sequences can serve as active foci for gene silencing in the mammalian genome.