PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR

摘要

PCR has become a powerful tool for genetic analysis and many applications for gene sequence quantitation are based on this technology (1-3), Standardized reaction conditions require accurate quantitation of input DNA as well as optimization of chemical and cycling parameters. In this study we discuss PicoGreen~(TM) (Molecular Probes, Eugene, OR) fluorescence enhancement as a useful assay for template DNA quantitation and PCR product formation.