Production of pectinase enzyme from cheap plant materials (corn pomace, rice bran and peanut chaff) using Aspergillus niger

摘要

The solid state fermentation of wastes (corn pomace, rice bran and peanut chaff) using Aspergillus niger for the production of pectinase was investigated. Total pectinase activity determined after five days showed that enzyme from corn pomace had thehighest total activity. The enzyme was used to clarify banana juice at different period of hydrolysis (incubation) of 1,2 and 3 hours respectively. The juices obtained were evaluated for yield, titratable acidity, pH, ascorbic acid, °Brix, viscosity turbidity and sugar content. Results indicate that the use of the enzyme was beneficial as it increased the yield of juice by 20%. There was a significant increase (p<0.05) in the acidity and °Brix value, while the value of viscosity and turbidity at 40°Cwas decreased after 3hours period of hydrolysis. However, there was no change in the value of the ascorbic acid and sugar content ofthe juice obtained.