Draft genome sequence and genetictransformation of the oleaginous algaNannochloropis gaditana

摘要

The potential use of algae in biofuels applications is receiving significant attention. However,none of the current algal model species are competitive production strains. Here we presenta draft genome sequence and a genetic transformation method for the marine microalgaNannochloropsis gaditana CCmP526. We show that N. gaditana has highly favourable lipid yields,and is a promising production organism. The genome assembly includes nuclear (~29 mb)and organellar genomes, and contains 9,052 gene models. We define the genes required forglycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limitedlipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism,including unique stramenopile photosynthesis genes and gene expansions that may explain thedistinguishing photoautotrophic phenotypes observed. The availability of a genome sequenceand transformation methods will facilitate investigations into N. gaditana lipid biosynthesis andpermit genetic engineering strategies to further improve this naturally productive alga.