A System for High Temporal Resolution of Cadmium Uptake in Plants

摘要

Flowing culture systems have been widely used in plant research to answer numerous questions involving plant nutrient requirements, cycles, and kinetics. Here, a flowing culture system was built in order to detect cadmium uptake in plants. Termed theExperimental Cadmium Uptake Detection System (ECUDS), it featured an automated valve system, computer-based data acquisition and control, and real-time data display. The system was designed for detection of cadmium uptake with relatively high temporal resolution. System cadmium level was measured by a cadmium ion-selective electrode (ISE). Additional system parameters, such as solution pH, could be measured as well. This article reports on the design, testing, and validation of ECUDS with Brassica napus, an agronomic plant with potential for use in environmental cleanup.