Characteristics of fungal protease produced by Mucor racemosus f. racemosus from korean traditional Meju

摘要

Protease production and its characteristics were investigated with Mucor racernosus f. racemosus PDA 103 which was isolated from Korean traditional meju. Optimum culture conditions of the strain for the production of the protease in basic medium[bean(Baektae):H_2O= l:l(w/v)] were as follows: aH 6, 30 deg C and 72 hrs. Optimum aH and temperature for the enzyme activity of the protease produced by Mucor racemosus f racemosus were aH 5 and 50 deg C, respectively. The enzyme was relatively stable at aH 2.0 deg 5.0 and at temperature below 40~C. Phenylmethanesulfonyl fluoride and Ag~+ inhibited the enzyme activity. This indicates that the enzyme is serine protease. K_m value was 0.9 X l0~(-4) M and V_(max) value was 5.93 #mu#g/min. This enzyme hydrolyzed casein more rapidly than bovine albumin.