Screening of biologically active essential oils from Ligusticum tenuissimum

摘要

The biological activities of the crude essential oils from Ligusticum tenuissimum and the control(phthalic anhydride) were compared. About 60 percent of the growth of MCF7, A549, and Hep3B cells were inhibited by adding 1.0 mg/ml of the crude essential oils and below 40 percent was observed by the control. Cytotoxicity on human normal lung cell(IMR90) was scored as 34.4 percent for the crude oil and 26.4 percent for control, respectively. It was found that the crude essential oils were more effective than the control in anti mutagenecity tested by both Rec-assay and ('HO V79 cells. The growth of human T-cell(Jurkat) was enhanced up to 1.21 times by adding the crude essential oil compared with the control. 50 percent of #alpha#-glucosidase activity was inhibited by both the crude essential oil and the control. ACE activities were inhibited 80.1 percent and 65.3 percent by adding 1.0 mg/ml of the crude oil and the control, respectively. The higher enhancement of glutathione-S-transferase activity was observed in the crude oil than those in the control: 301 percent v.s 234 percent at 1.0 mg/ml of the treatment. Thrombolytic activity was measured as 42.9 percent and 28.6 percent for the crude oil and the standard, respectively. The effect of the oil on the nerve cells PC12, was observed as follows: the neurite of PC12 cells was lengthened up to 255 #mu#m longer than 205 #mu#m of control. The number of neurite-bearing cells were about two times higher than control. The survival ratio of the crude essential oil was also increased up to 56.4 percent which was about two fold higher than in control.