Cloning and Expressionof the Mouse PIP49 (Pancreatitis Induced Protein 49) mRNA Which Encodes a New Putative Transmembrane Protein Activated in the Pancreas with Acute Pancreatitis

摘要

We have used a microarray-based strategy to char-acterize, at the molecular level, the pancreatic emer-gency program set up by the pancreatic cells in re-sponse to pancreatitis. In this strategy, the phenotype of the pancreatitis-affected pancreas is established by characterization of a large number of its transcripts using a high-density mouse cDNA microarray. This method allows identification of transcripts differen-tially expressed during pancreatitis. We describe here the cloning, sequencing, and expression analysis of a new gene, named P1P49 (Pancreatitis Induced Protein 49). Its very strong expression is specific of acinar cells and occurs rapidly after initiation of the acute phase of pancreatitis. Analysis of its primary and secondary structures strongly suggests that P1P49 encodes a pu-tative transmembrane protein.