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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Influence of 1.8-GHz (GSM) radiofrequency radiation (RFR) on DNA damage and repair induced by X-rays in human leukocytes in vitro.
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Influence of 1.8-GHz (GSM) radiofrequency radiation (RFR) on DNA damage and repair induced by X-rays in human leukocytes in vitro.

机译:1.8 GHz(GSM)射频辐射(RFR)对X射线诱导的人白细胞体外DNA损伤和修复的影响。

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摘要

In the present study, the in vitro comet assay was used to determine whether 1.8-GHz radiofrequency radiation (RFR) can influence DNA repair in human leukocytes exposed to X-rays. The specific energy absorption rate (SAR) of 2 W/kg (the current European safety limit) was applied. The leukocytes from four young healthy donors were intermittently exposed to RFR for 24 h (fields on for 5 min, fields off for 10 min), and then irradiated with X-rays at doses of 0.25, 0.5, 1.0 and 2.0 Gy. DNA damage to human leukocytes was detected using the comet assay at 0, 15, 45, 90, 150 and 240 min after exposure to X-rays. Using the comet assay, the percent of DNA in the tail (% tail DNA) served as the indicator of DNA damage; the DNA repair percentage (DRP) served as the indicator of the DNA repair speed. The results demonstrated that (1) the DNA repair speeds of human leukocytes after X-ray exposure exhibited individual differences among the four donors; (2) the intermittent exposures of 1.8-GHz RFR at the SAR of 2 W/kg for 24 h did not directly induce DNA damage or exhibit synergistic effects with X-rays on human leukocytes.
机译:在本研究中,体外彗星试验用于确定1.8 GHz射频辐射(RFR)是否可以影响暴露于X射线的人白细胞的DNA修复。能量吸收率(SAR)为2 W / kg(当前的欧洲安全限值)。将来自四个年轻健康供体的白细胞间歇性地暴露于RFR 24小时(场开5分钟,场关10分钟),然后以0.25、0.5、1.0和2.0 Gy的剂量照射X射线。暴露于X射线后0、15、45、90、150和240分钟,使用彗星试验检测对人类白细胞的DNA损伤。使用彗星试验,尾巴中的DNA百分比(尾巴DNA百分比)可作为DNA损伤的指标; DNA修复百分比(DRP)是DNA修复速度的指标。结果表明:(1)X射线照射后人白细胞的DNA修复速度在四个供体之间表现出个体差异; (2)在SAR为2 W / kg的条件下,1.8 GHz RFR在SAR下间歇性暴露24 h不会直接诱导DNA损伤或与X射线对人白细胞产生协同作用。

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