Application of Molecular Markers in Predicting Production Quality ofCultivated Cistanche deserticola

摘要

We developed a set of molecular markers in Cistanche deserticola Y. C. MA to evaluate the production qual-ity of cultivated C. deserticola individuals. This application utilizes the inter-simple-sequence repeat (ISSR) poly-merase chain reaction (PCR) and random amplified polymorphic DNA (RAPD) PCR as molecular markers todetermine the echinacoside content in cultivated C. deserticola individuals. The unweighted pair-group methodusing arithmetic average clustering (UPGMA) confirmed that the combined ISSR and RAPD data could catego-rize all C. deserticola individuals into three groups according to their respective echinacoside content. The step-wise multiple regression analysis (SMRA) revealed six potential markers associated with echinacoside accumula-tion in C. deserticola and produced 18 echinacoside-marker prediction models, four of which were successfullyused to predict the quality of C. deserticola from Neimenggu. Both clustering and SMRA showed a correlationbetween the echinacoside content and molecular markers in cultivated C. deserticola. The relative average devia-tion of prediction (RADP) of the prediction models could be improved by simplifying and adjusting the model. Itwas found that the RADP value could reach 2.6% after adjustment and the simplified prediction models couldsuccessfully predict the quality of cultivated C. deserticola individuals.