Primary biliary cirrhosis sera recognize not only gp210 but also proteins of the p62 complex bearing N-acetylglucosamine residues from rat liver nuclear envelope Anti-p62 complex antibody in PBC

摘要

We have recently observed reactivity of primary biliary cirrhosis (PBC) sera with several proteins bearing N-acetylglucosamihe residues from rat liver nuclear envelopes, The aim of this study was to characterize the reactive antigens. Sera from 31 patients with PBC, 30 with rheumatoid arthritis (RA) and 30 with Sjogren's syndrome (SS) were examined. Rim-like immunofluorescence staining was observed in 15 of 31 (48%) sera froni patients, with PBC, in 1 of 30 with RA and in 1 of 30 with SS. Upon immunoblotting using preparations of whole rat liver nuclear envelopes and their Triton X 100-KC1 extract as antigen sources, a 200 kDa protein band was observed in 9 of sera with PBC, Furthermore, upon immunoblotting using the wheat germ aggulutinin-bound fraction at rat liver envelope as antigen, 62, 60 and 54 kDa protein bands corresponding to components of the p62 complex in the nuclefir ptire complex (Kita et al, Biochem, 113, 377-382) were observed in 7, 5 and 6 samples respectively, of the 31 PBC sera. Our ciata suggest that PBC sera recognize not only the 210 kDa protein but also the p62 complex proteins.