Morphometrical analysis of neurons of the amygdaloideum's nucleus corpus amygdaloideum baso-lateralis.

摘要

The aim of the study was to quantitatively observe (morphometrical analysis) neurons during the maturation process of amygdaloideum baso-lateralis nuclei. The brains of domestic pigs of both sexes (obtained from the following periods: weeks 7-15 week of intrauterine life, newborn animals, 1-month-old and 1-year-old animals) were examined. The brains were removed and examined conventionally by the microscope. The preparations were dyed according to Kltivera-Barrer's method as well as the Nissel method. The histological preparations obtained were used to morphometrically analyze the neurons of the corpus amygdaloideum's nucleus amygdaloideum baso-lateralis. Morphometrical examinations were carried out using the Nikon Eclipse E-600 microscope compressed with a JVC TK-1380-E camera and a computer with the MULTI-SCAN-BASE 08.98 morphometrical programme. The following parameters were examined: the cross-section of nerve cells and the area of cell nuclei in micro m2, the percentage of the nuclei-cell rate, and the average diameter of nervous cell in micro m, the volume of nervous cell in micro m3, the number of neurons per 1 mm2, and the number of neurons per 1 mm3. Morphometrical observations revealed that cells forming the primary corpus of amygdaloideum in week 9 of fetal life were identical in shape and size. The size of the cell area at week 9 of fetal life was ~45 micro m2. These cells, homogenous in the previous periods, underwent considerable differentiation during week 11 of fetal life. Mass nucleus structures appeared from homogenous cells including the amygdaloideum baso-lateralis nuclei. The examined morphometrical parameters revealed that neurons become morphologically mature at the end of fetal life..