Protective effect of a Lactobacillus salivarius strain of human origin.

摘要

The protective effect of a Lactobacillus salivarius strain from human faeces (HA8) was evaluated against the cytotoxicity of N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR), N-nitrosodibutylamine (NDBA) and N-nitrosopiperidine (NPIP) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. L. salivarius HA8 strain showed a moderate protective effect against NPYR and weak against NDBA and NPIP. No protective effect against cytotoxicity of NDMA was observed at the bacterial population used. To test the effect of L. salivarius HA8 on cytokine production (interleukin-1 beta , IL-1 beta ; interleukin-8, IL-8; and tumour necrosis factor alpha, TNF- alpha ), the human macrophage cell line (THP-1) was cultured in the presence and absence of lipopolysaccharide (LPS). L. salivarius HA8 induced IL-1 beta , IL-8 and TNF- alpha releases when cells were stimulating with and without LPS..