DETECTION OF SALMONELLA SPP., LISTERIA MONOCYTOGENES AND CRONOBACTER SPP. IN PRODUCTS OF ANIMAL ORIGIN USINGREAL-TIME PCR BASED KITS

摘要

Hundreds of foodborne infections occur throughout the world each year. Food products, such as milk and meat, are susceptible to contamination by pathogenic bacteria, including Salmonella spp., Listeria monocytogens and, in recent years, also Cronobacter spp. Traditional methods for the detection of these bacteria are laborious and time-consuming. However, DNA analysis-based technologies made the development of alternative tests for the detection of microbial pathogens feasible. In this study, ready-to-use kits for the next-day detection of L. monocytogenes, Salmonella spp. and Cronobacter spp. in foods were designed based on PCR, mostly developed in our laboratory, and evaluated for their selectivity, detection limit and applicability.