Getting the Most Out of Quantitative PCR: Strategies and Solutions for Overcoming Common Problems in Laboratory Experiments

摘要

The enhancement of PCR with realtime quantitative methods takes PCR from a workhorse laboratory preparatory method for molecular biology to an extremely powerful diagnostic and analysis tool for clinical and research applications. Studies of gene expression are some of the most common applications of qPCR. It is also increasingly used for clinical diagnostic testing. The technique is sensitive enough to detect DNA or RNA from an infectious disease, or even early stages of cancer.