Molecular characterization of structural barley mutants produced by gamma-irradiation

摘要

Various molecular marker systems, including STS, CAB REMAP, RAPD and AFLP were employed to screen for DNA altera-tions in the genomes of thirteen original barley structural mutants pro-duced by gamma-irradiation. Two out of total 4888 fragments generated in the mutant forms by the applied set of markers were polymorphic which indicate a relatively low occurrence of stable radiation-induced genetic variations in the resulting mutant lines. Among the marker sys-tems AFLP was proven to be the most appropriate for the analysis of radiation-induced DNA alterations in barley. AFLP analysis revealed sequence polymorphism in 2 out of the 13 translocation lines analysed. As far as gross chromosomal rearrangements such as translocations and inversions concern large chromosome domains, the respective specific DNA sequences analysed may not completely cover the areas where subtle mutational changes may have occurred in the mutant genotypes produced. The data obtained in the present study support the notion for the relatively high genetic stability of barley genome.