Engineering High Catalytic Efficiency of the Steroid Isomerase Activity of Human Glutathione S-transferase P1-1

摘要

Glutathione S-transferases (GSTs) are a family of multifunctional enzymes that catalyze the formation of conjugates between reduced glutathione (GSH) and a variety of carcinogenic, mutagenic, toxic and pharmacologically active compounds.1'2 GSTs have also been shown to participate in other biological process. Certain GSTs can detoxify lipid and DNA hydroperoxide by their intrinsic peroxidase activity, while others can catalyze the isomerization of certain steroids. Mammalian cytosolic GSTs, which exist as homo- or hetero-dimers, are grouped into at least seven distinct classes -alpha, mu, pi, sigma, theta, sigma, kappa and zeta - according to their physical, chemical, immunological and structural properties.