Effects of water-soluble oligosaccharides extracted from lotus (Nelumbo nucifera Gaertn.) seeds on growth ability of Bifidobacterium adolescentis

摘要

Many microorganisms including some probiotic species in human gastrointestinal tract are sensitive to gastric acid secretions. The use of simulated gastrointestinal environments is a suitable approach to study the effects of a single factor on the stability of probiotics in the gastrointestinal tract (GIT). In this study, the growth effects of lotus seed oligosaccharides and purified lotus seed oligosaccharides on Bifidobacterium adolescentis were assessed in a simulated gastrointestinal tract environment. Compared to glucose (Glc), xylo-oligosaccharides (XOS), and fructooligosaccharides (FOS), lotus seed oligosaccharides (LOS), and purified lotus seed oligosaccharides (P-LOS) are more effective on promoting the growth of B. adolescentis. The final bacterial mass was higher in LOS and P-LOS than in Glc. The viability of B. adolescentis incubated at pH 1.5, 2.0, or 3.0 was improved by LOS and P-LOS. Additionally, LOS and P-LOS increased the viability of B. adolescentis in bile salts up to 10 g/L and in digestive juices. The results revealed that LOS is an effective growth-accelerating factor of B. adolescentis, which improves the viability of B. adolescentis in gastrointestinal conditions.