Isolation of natural inhibitors of papain obtained from Carica papaya latex.

摘要

Studies were conducted to isolate natural inhibitors of papain obtained from C. papaya latex. Fresh latex from green fruits was collected and 3 fractions with the inhibitor were isolated using latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephandex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin. However, the fraction identified as P-III showed positive reaction with ninhydrin, confirming its peptidic nature. Kinetics data showed non-competitive inhibition (inhibitor I) and incompetitive inhibition (inhibitor II and P-II).