Micropropagation and Genetic Stability of Pyracantha fortuneana, Roem. Shrub

摘要

THIS STUDY was carried out in Plant Tissue Culture Laboratory at El-Zohria Botanical Garden, Horticulture Research Institute, Agriculture Research Center during 2007-2008 seasons. The object of this study was to establish a protocol for micropropagation and RAPD analysis for comparing between mother plant and in vitro plant of Pyracantiia fortuneana Roem. The data revealed that for sterilization explant, using 40% clorox for 25 min gave the best result (100% survival) and free contamination explant.Bud break and shoot development were best on full MS establishment medium with (2.0 mg/1 IBA) which gave the longest shootlet. While the greatest number of leaves was recorded on full MS-medium with (4.0 mg/ IBA). For shooting behaviour, the medium supplemented with (3.0 mg/I BA) for four subcultures gave the greatest number of shootlet. But adding (4.0 or 5.0 mg/1 kin) for four subcultures produced the tallest shootlet length. For the number of leaves, culture the explant on MS-medium have (4.0 mg/1 BA) for four subcultures gave the highest number of leaves. Regarding to the rooting behaviour, culturing the shootlet on MS-medium supplemented with (4.0 mg/l IBA) produced the highest percentage of rooting, tallest root length and highest number of root.RAPD-based DNA fingerprints gave no evidence of somaclonal variation during the in vitro propagation protocol used. The RAPD profiles of tissue culture-derived plantlets revealed high similarity to mother tree. For acclimatization, the survival percentage was 70%.