Micropropagation of Lavandula officinalis L. through shoot tip culture of mature plants

摘要

Shoot-tip explants of mature greenhouse-grown Lavandula officinalis L plant were inoculated aseptically on Murashige and Skoog's (MS) medium supplemented with different concentrations of 6-benzylaminopurine (BAP). The optimum concentration of BAP was4.5 #mu#M for shoot proliferation. After elongation of the shoots, the axillary buds developed and formed new shoots which excised and subcultured on multiplication media. For shoot multiplication, the concentration of 4.5 #mu#M BAP was found to be theoptimum. High rate of rooting (94.11 percent) was obtained with 9.8#mu#M of 3-indolebutyric acid (IBA). The rooted plantlets were acclimatized in a controlled environment growth chamber with 80 percent of survival plants. Plantlets were successfully transferred to the greenhouse and showed normal morphology when grown to maturity.