The significance of a negative map ELISA test for Mycobacterium avium subspecies paratuberculosis.

摘要

The significance of a negative USDA certified Map ELISA test was assessed in three separate study designs. * Part I: The sera of seven out of nine necropsied cows with documented Johne's disease and whose feces was positive for mycobacterium DNA tested negative using the ParaChekReg. Map ELISA test system (Prionic, Switerland). * Part II: Of the sera from 42 cows whose fecal specimens had been characterized by the TrekReg. Diagnostic System as having a significant amount of Map, 39 specimens tested negative in the ParaChekReg. test. When serological testing was extended for 14 additional months, 20 of the 21 animals remained sero-negative. * Part III: Comparative serological analysis done on the same serum samples from a dairy herd demonstrated that 42% of the cows test certified as having been Map-free had anti-Map antibodies.