Lectin purified from Musca domestica pupa up-regulates NO and iNOS production via TLR4/NF-kappaB signaling pathway in macrophages.

摘要

The present study reported that nitric oxide (NO) was up-regulated by the induction of lectin purified from Musca domestica pupa (MPL) in macrophages without cytotoxicity. The mRNA expression and protein secretion of inducible nitric oxide synthase (iNOS) were strongly induced by MPL treatments. Subsequent investigation revealed that the nuclear factor-kappaB (NF-kappaB) inhibitory kappaB (IkappaB) in endochylema was inhibited and NF-kappaB translocated into the nucleus after MPL treatment. Meanwhile, the IKKbeta was strongly induced and the production of the toll-like receptor 4 (TLR4) was significantly up-regulated. Moreover, MPL increased NO production via inducing the expression of iNOS through the activation of NF-kappaB, which suggested that MPL probably acted as an activating agent of the NF-kappaB activation.